The Combined Effect of Two Natural Extracts on Enamel Remineralization (in vitro Study)

Autori

DOI:

https://doi.org/10.5195/d3000.2024.712

Parole chiave:

Caseins, Eggshell, Enamel, Grape Seed Extract, Tooth Remineralization

Abstract

Objective: Dental caries is a chronic infectious disease that is prevalent worldwide in all age groups. Numerous attempts have been made to develop conservative approaches to halt caries progression and restore enamel defects. This study aimed to investigate the effect of applying grape seed extract and chicken eggshell extract on the microhardness of demineralized enamel in permanent teeth.

Methods: Forty-eight sound upper first premolars were used. Following demineralization with the demineralizing solution for 96 hours, they were distributed into four groups consistent with the treatment agent used: group A was treated with casein phosphopeptide amorphous calcium phosphate (as a control group), group B was treated with grape seed extract, group C was treated with chicken eggshell extract solution, and group D was treated with grape seed extract followed by chicken eggshell extract solution. Vickers microhardness measurements were performed on sound enamel, after demineralization and remineralization.

Results: Paired T-test, one-way ANOVA, and Tukey’s HSD test were used for statistical analysis. Enamel microhardness was significantly reduced following demineralization (p=0.000) and significantly increased after remineralization, with group D showing the highest values (mean microhardness=218.99).

Conclusion: Grape seed extract and chicken eggshell extract solutions have a synergistic effect on enamel remineralization which was interpreted from the increase in surface microhardness values.

Biografie autore

Alhan Qasim, College of Dentistry, University of Baghdad

Assistant  Professor, Department of Pediatric and Preventive Dentistry

Rusul Hadi, College of Dentistry, University of Baghdad

Assistant Lecturer, Department of Oral Diagnosis

Asmaa M. Khammas, College of Dentistry, University of Baghdad

Assist Lecturer, Department of Pediatric and Preventive Dentistry

Riferimenti bibliografici

Obaid SF, Munther S, Abbas MH. Relationship between Salivary Levels of Protein Carbonyl and Total Antioxidant Capacity and Prevalence of Dental Caries among Type 1 Diabetic Children: An Analytical Cross-Sectional Study. Dental Hypotheses. 2023;14(2):59. DOI: 10.4103/denthyp.denthyp_44_23.

Arifa MK, Ephraim R, Rajamani T. Recent advances in dental hard tissue remineralization: a review of literature. International journal of clinical pediatric dentistry. 2019;12(2):139. doi: 10.5005/jp-journals-10005-1603

Attar IJ, Ghaib NH. The effect of some remineralization materials and resin infiltration (ICON) on the depth of white spot lesions. J Bagh Coll Dentistry. 2018;30(2). https://doi.org/10.12816/0049755

Reema SD, Lahiri PK, Roy SS. Review of casein phosphopeptides-amorphous calcium phosphate. Chin J Dent Res. 2014;17(1):7-14. PMID: 25028684

Turki OH, Jafar ZJ. Whitening Effect of Juglans Regia Dry Husk Extract on Primary and Permanent Teeth. International Journal of Biomaterials. 2023;2023.

Park JW, Bae SR, Suh JY, Lee DH, Kim SH, Kim H, et al. Evaluation of bone healing with eggshell‐derived bone graft substitutes in rat calvaria: A pilot study. Journal of Biomedical Materials Research Part A. 2008;87(1):203-14.

Kadhim DR, Hamad TI, Fatalla AA. Use of Eggshells as Bone Grafts around Commercially Pure Titanium Implant Screws Coated with Nano Calcium Sulfate. International Journal of Biomaterials. 2022;2022. https://doi.org/10.1155/2022/8722283

Haghgoo R, Mehran M, Ahmadvand M, Ahmadvand MJ. Remineralization effect of eggshell versus nano-hydroxyapatite on caries-like lesions in permanent teeth (in vitro). Journal of International Oral Health. 2016;8(4):435.

Yaberi M, Haghgoo R. A comparative study of the effect of nanohydroxyapatite and eggshell on erosive lesions of the enamel of permanent teeth following soft drink exposure: A randomized clinical trial. Journal of International Oral Health. 2018;10(4):176. DOI:10.4103/jioh.jioh_84_18

Mohamed EO, Sharaf AA, Talaat DM, Nagui DA. Remineralization effect of eggshell powder and novamine on initial caries-like lesions in young permanent teeth (in-vitro study). Alexandria Dental Journal. 2020;45(2):52-9.

Delimont NM, Carlson BN. Prevention of dental caries by grape seed extract supplementation: A systematic review. Nutrition and health. 2020;26(1):43-52. DOI: 10.1177/0260106019887890

Hassan NM, Jafar ZJ, Abdul Latif MH. Nano‐hydroxyapatite preparation for the remineralization of primary tooth enamel surface subjected to liquid medication: An observational study. Health Science Reports. 2023;6(4):e1188.

Yahya Y, Alrawi N. Remineralizing Efficacy of Biomimetic Self-Assembling Peptide on Artificially Induced Enamel Lesions (In Vitro Study). J Res Med Dent Sci,. 2020;8 (7): 407-12.

Faul, F., Erdfelder, E., Buchner, A., & Lang, A.-G. Statistical power analyses using G*Power 3.1: Tests for correlation and regression analyses. Behavior Research Methods. 2009;41: 1149-1160.

Cohen j. Statistical power analysis for the behavioral sciences. 1988 (2nd ed) (Hillsdale, NJ:Erlbaum).

Poggio C, Lombardini M, Vigorelli P, Ceci M. Analysis of dentin/enamel remineralization by a CPP‐ACP paste: AFM and SEM study. Scanning: The Journal of Scanning Microscopies. 2013;35(6):366-74.

Tang CF, Fang M, Liu RR, Dou Q, Chai ZG, Xiao YH, Chen JH. The role of grape seed extract in the remineralization of demineralized dentine: micromorphological and physical analyses. Archives of oral biology. 2013 Dec 1;58(12):1769-76. DOI: 10.1016/j.archoralbio.2013.09.007

Saragih D, Herda E, Triaminingsih S, editors. The influence of topical application of grapeseed extract gel on enamel surface hardness after demineralization. Journal of Physics: Conference Series; 2017: IOP Publishing.

Suzuki F, Wang P-Y., Weatherspoon J, Mead L. Method of producing eggshell powder. Patents: US 20060062857 A1. 2004.

Feroz S, Moeen F, Haq SN. Protective Effect of Chicken Egg Shell Powder Solution (CESP) on Artificially Induced Dental Erosion: An in Vitro Atomic Force Microscope Study. International Journal of Dental Sciences and Research. 2017;5(3):49-55. DOI:10.12691/ijdsr-5-3-2

Itthagarun A, Verma S, Lalloo R, King N, Wefel J, Nair R. Effects of fluoridated milk on artificial enamel carious lesions: a pH cycling study. Journal of dentistry. 2011;39(12):817-24.

Leal SC, Dame-Teixeira N, Barbosa CdB, Kominami PAA, Raposo F, Nakagawa EMT, et al. Minimum intervention oral care: defining the future of caries management. Brazilian Oral Research. 2022;36. DOI: 10.1590/1807-3107bor-2022.vol36.0135.

Obeid RS, Khalaf MS. Impact of different types of storage media on enamel surface roughness and granularity distribution of avulsed teeth (In vitro study). J Bagh Coll Dentistry. 2022;34(4):34-43.

Cheng L, Li J, Hao Y, Zhou X. Effect of compounds of Galla chinensis on remineralization of enamel surface in vitro. Archives of oral biology. 2010;55(6):435-40. DOI: 10.1016/j.archoralbio.2010.03.014.

Rubel M, Prashant GM, Naveen Kumar PG, Sushanth VH, Imranulla M, Potlia I, Mallick S. Effect of grape seed extract on remineralization of artificial caries: An in-vitro study. Asian J Pharm Clin Res. 2016;9(5):174-6.

Mirkarimi M, Eskandarion S, Bargrizan M, Delazar A, Kharazifard MJ. Remineralization of artificial caries in primary teeth by grape seed extract: An in vitro study 2013;7(4):206-10.

Yassen AA, Safy RK. Grape seed extract and dentin remineralization. Egyptian Dental Journal. 2018;64:1719-26.

Amin RA, Awad SM, Abd El Sattar EA. Evaluation of remineralization of grape seed extract versus sodium fluoride on demineralized primary anterior teeth: An in-vitro study. Egyptian Dental Journal. 2019;65: 1977-84.

Tangboriboon N, Kunanuruksapong R, Sirivat A. Preparation and properties of calcium oxide from eggshells via calcination. Materials Science-Poland. 2012;30(4):313-22. https://doi.org/10.2478/s13536-012-0055-7.

Elshik AE, Etman WM, Genaid TM. Resistance of chicken eggshell powder-treated demineralized enamel to further acidic challenges. Tanta Dental Journal. 2020;17(2):38.

Magalhães AC, Moron B, Comar L, Wiegand A, Buchalla W, Buzalaf MAR. Comparison of cross-sectional hardness and transverse microradiography of artificial carious enamel lesions induced by different demineralizing solutions and gels. Caries research. 2009;43(6):474-83. doi: 10.1159/000264685

Ren Y-F, Zhao Q, Malmstrom H, Barnes V, Xu T. Assessing fluoride treatment and resistance of dental enamel to soft drink erosion in vitro: applications of focus variation 3D scanning microscopy and stylus profilometry. journal of dentistry. 2009;37(3):167-76.

Hemingway C, Parker D, Addy M, Barbour M. Erosion of enamel by non-carbonated soft drinks with and without toothbrushing abrasion. British Dental Journal. 2006;201(7):447-50. PMID: 17031352

##submission.downloads##

Pubblicato

2024-10-03

Fascicolo

V. 12 N. 2 (2024)

Sezione

Adults & the Elderly

Licenza

Copyright (c) 2024 Shatha A. Abbas, Alhan A. Qasim, Rusul J. Hadi, Sarah M. Khammas, Asmaa M. Khammas

Creative Commons License

TQuesto lavoro è fornito con la licenza Creative Commons Attribuzione 4.0 Internazionale.

Authors who publish with this journal agree to the following terms:

  1. The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.
  2. Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.
  3. The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a Creative Commons Attribution 4.0 International License or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:
    1. Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;
    with the understanding that the above condition can be waived with permission from the Author and that where the Work or any of its elements is in the public domain under applicable law, that status is in no way affected by the license.
  4. The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.
  5. Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Any such posting made before acceptance and publication of the Work shall be updated upon publication to include a reference to the Publisher-assigned DOI (Digital Object Identifier) and a link to the online abstract for the final published Work in the Journal.
  6. Upon Publisher’s request, the Author agrees to furnish promptly to Publisher, at the Author’s own expense, written evidence of the permissions, licenses, and consents for use of third-party material included within the Work, except as determined by Publisher to be covered by the principles of Fair Use.
  7. The Author represents and warrants that:
    1. the Work is the Author’s original work;
    2. the Author has not transferred, and will not transfer, exclusive rights in the Work to any third party;
    3. the Work is not pending review or under consideration by another publisher;
    4. the Work has not previously been published;
    5. the Work contains no misrepresentation or infringement of the Work or property of other authors or third parties; and
    6. the Work contains no libel, invasion of privacy, or other unlawful matter.
  8. The Author agrees to indemnify and hold Publisher harmless from Author’s breach of the representations and warranties contained in Paragraph 6 above, as well as any claim or proceeding relating to Publisher’s use and publication of any content contained in the Work, including third-party content.

Revised 7/16/2018. Revision Description: Removed outdated link.