Histological Evaluation of Cutaneous Wound Healing Treated by Local Application of Cucurbita pepo L. Seed Oil

نویسندگان

DOI::

https://doi.org/10.5195/d3000.2025.821

کلمات کلیدی:

Cucurbita pepo L. oil, Wound healing, Natural product

چکیده

Objective: The rising demand for natural medicines in pharmaceuticals has prompted scientists to investigate medicinal plants. Pumpkin seed (Cucurbita pepo L.) is a noteworthy candidate due to its remarkable pharmacological qualities for possible wound healing therapies.

Methods: A total of twelve mature male New Zealand rabbits weighing between 1.5 and 2 kg were subjected to uniform wounds on their dorsum. These rabbits were then split into two groups for healing durations of 3 and 7 days, with 6 rabbits in each group. The induced wound in the control group was allowed to heal naturally, whereas the wounds in the experimental group were treated with a daily local application of pumpkin oil (10µl). The animals were sacrificed after healing times of three and seven days. All examined groups underwent clinical assessment of wound contraction. Specimens were obtained for histological and histomorphometric analysis to evaluate inflammatory cell and blood vessel counts, as well as epithelial thickness.

Results: The average percentage of wound contraction escalated over time. A notable change was found and a statistically significant disparity in the mean values of inflammatory cells was recorded. The mean values for blood vessel count rose with a notable disparity was noted between the control and experimental groups. The evaluation of epithelial thickness indicated that mean values increased over time, with a significant difference in both experimental groups.

Conclusion: As a potential therapeutic agent for wound healing, pumpkin oil may be also beneficial for nutritional and therapeutic applications.

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چاپ شده

2025-02-17

شماره

دوره 13 شماره 1 (2025)

نوع مقاله

Mechanisms of Oral Disease

مجوز

حق نشر 2025 Ibrahim Fouad Mohamed, Ban A. Ghani , Nada M.H. Al-Ghaban , Abdalbseet A. Fatalla , Matheel Al-Rawas

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