Evaluation of Salivary and Oral Cell Collection Methods for Genomic DNA Extraction

Συγγραφείς

  • Clarissa Christina Avelar Fernandez Universidade Federal do Rio de Janeiro https://orcid.org/0000-0002-2786-4146
  • Fernanda Farage da Costa Felipe Ferreira Universidade Federal do Rio de Janeiro
  • Christiane Vasconcellos Cruz Universidade Federal do Rio de Janeiro
  • Marcelo de Castro Costa Universidade Federal do Rio de Janeiro

DOI:

https://doi.org/10.5195/d3000.2019.91

Λέξεις-κλειδιά:

DNA, Saliva, Genetics

Περίληψη

The use of saliva and oral cells as sources of biological material has gained attention, due to advantages such as facility, non-invasiveness, and great patient acceptance. The objective of the study was to compare four different types of saliva and oral buccal cell collecting methods for genomic DNA extraction: (1)Expectoration of saliva, (2)Expectoration of saliva with lingual stimulation, (3)Scraping with cytological brush, and (4)Scraping with cytological brush and expectoration of saliva. The sample was composed of students and employees from the Dental School of the Federal University of Rio de Janeiro (n = 20, 10 men and 10 women with mean age of 47.60 ± 15.70 and 20.50 ± 2.1, respectively). The collections were performed with an interval of at least one day between them and the participants were instructed to stay for less than 30 minutes without eating food and brushing teeth. Samples were stored at -20°C until DNA extraction was performed using a commercially available kit (Qiagen®). Differences in DNA yield between methods were test for statistical significance with an alpha of 0.05. No sexual dimorphism was observed in relation to the concentration of DNA (p=0.76), age (p=0.91), and ethnicities (p=0.72). There was no significant difference between the collection methods in relation to the quantity and purity of the extracted DNA (p≥0.05). All methods gave lower DNA yields than the ones obtained from blood or saliva collected through comercial kits and maybe carefully use forclinical diagnostic purposes or for research experiements requiring higher DNA concentrations.

Αναφορές

Avaliação do rendimento, pureza e integridade do DNA genômico em diferentes protocolos de coleta de células bucais. Waltrick-Zambuzzi MR, Vieira, TCS, Romanos HF, Macedo EE, Granjeiro JM, Kuchler EC. Int J Dent, Recife. 2012 jan/mar; 11(1):12-18.

Comparison of different collection procedures and two methods for DNA isolation from saliva. Durdiaková J, Kamodyivá N, Ostatníková D, Vlková B, Celec P. Clin Chem Lab Med. 2012; 50(4):643–647.

Determinants of DNA yield and purity collected with buccal cell samples. Van Wieren-de Wijer DBMA, Maitland-van der Zee AH, de Boer A, Belitser SV, Kroon AA, de Leeuw PW, Schiffers P, Janssen RGJH, van Duijn CM, Stricker BHCH, Klungel OH. Eur J Epidemiol. 2009; 24: 677-682.

Comparison of methods for collection of DNA samples by mail in the black women´s helth study. Cozier YC, Palmer JR, Rosenberg L. Ann Epidemiol. 2004; V. 14, P.117-122.

DNA yeld and quality of saliva samples and suitability for large-scale epidemiological studies in children. Koni AC, Scott RA, Wang G, Bailey MES, Peplies J, Bammann K, Pirsiladis YP. Internt Journ of Obes. 2011; V. 35. P 113-118.

Diagnostic potential of saliva: current state and future applications. Pfaffe T, Cooper-White J, Beyerlein P, Kosner K, Punyadeera C. Clinc Chem. 2011; V. 57. P 675-687.

Quality and quantity of saliva DNA obtained from the self-administrated oragene method - a pilot study on the cohort of swedish men. Rylander-Rudqvist T, Hankansson N, Tybring G, Wolk, Alicja. Cancer Epidemiol Biomarkers Prev. 2006;15(9).

Comparative study of five commercially avaiable saliva collection kits for DNA extraction. Deeley K, Noel J, Vieira ARClin Lab. 2016; 1;62(9):1809-1813.

Collection of buccal cell DNA in seventh-grade children using water and a toothbrush. London SJ, Xia J, Lehman TA, Yang J, Granada E, Chunhong L, Dubeau L, Li T, David-Beabes GL, Li Y. Canc Epid Biom Prev, 2001; v.10, p.1227-1230.

Quality of DNA extracted from saliva samples collected with the Oragene DNA self- collection kit. Nuner AP, Oliveira IO, Santos BR, Millech C, Silva LP, González DA, Hallal PC, Menezes AMBB, Araújo CL, Barros F. Med Reserch Method. 2012.

Collection of blood, saliva and buccal cell samples in a pilot study on the danish nurse cohort: comparison of the response rate and quality of genomic DNA. Hansen TO, Simonsen MK, Nielsen FC, Hundrup YA. Cancer Epidemiol Biomarkers Prev. 2007; V. 16. P.2072-2076.

Buccal DNA collection: comparison of buccal swabs with FTA cards. Milne E, Bockxmeer FMV, Robertson L, Brisbane JM, Ashton LJ, Scott RJ, Armstrong BK. Canc Epid Biom Prev. 2006; V.15. P. 816-819.

Λήψεις

Δημοσιευμένα

2019-07-25

Τεύχος

Τόμ. 7 Αρ. 1 (2019)

Ενότητα

Development of Craniofacial Structures

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