Relation of Amelogenin Gene Polymorphisms to Dental Caries Among Iraqi Teenagers

Συγγραφείς

  • Elaf Abdulkareem Alidan Lectural, Department of Pediatric, Orthodontic and Preventive Dentistry, College of Dentistry/University of Ashur, Iraq.
  • Ahlam Taha Mohammed
  • Batool Hassan Al-Ghurabi

DOI:

https://doi.org/10.5195/d3000.2025.842

Λέξεις-κλειδιά:

AMELX, Dental Caries, Health Risk, Amelogenin, Single Nucleotide Polymorphisms

Περίληψη

Objective: To examine the relationship between dental caries and single nucleotide polymorphisms (SNPs) in AMELX gene.

Methods: This study was a comparative cross- sectional study encompassing 78 adolescents (aged 13-15 years) was conducted. All of them were examined for their oral and dental status under the WHO recommended criteria, and clinical information such as DMFT and DMFS were evaluated. Individuals whose DMFT and DMFS index lower than 2 were designated 'very low caries experience' and higher than 9 were designated 'higher caries experience'. Genomic DNA was extracted from saliva samples, and single nucleotide polymorphisms of AMELX were genotyped. Genotyping of three SNPs (rs17878486, rs5933871, rs5934997, intron) in AMELX gene was determined by direct sequencing and analyzed with SNPStats. Chi-square was used to compare allele and genotype frequencies between cases and controls.

Results: There was no significant difference between the high and low caries groups but in high caries group, it showed a deviation from Hardy-Weinberg equilibrium with a significant difference among the three genotypes (p=0.013) in rs5933871 and rs5934997, while a highly significant difference in rs17878486 for both high and low caries groups (p=0.000 and p=0.001). A highly significant and positive correlation between rs17878486 with DMFS in the control group and a positive significant correlation between rs17878486 and DMFT.

Conclusion: Although no significant difference of the investigated SNPs of AMELX between high and low caries groups, but rs17878486 had a significant and positive correlation with DMFT and DMFS which made this SNP as marker for caries susceptibility.

Λήψεις

Δημοσιευμένα

2025-05-02

Τεύχος

Τόμ. 13 Αρ. 1 (2025)

Ενότητα

Infancy & Adolescence

Άδεια

Πνευματική ιδιοκτησία (c) 2025 Elaf Abdulkareem Alidan, Ahlam Taha Mohammed, Batool Hassan Al-Ghurabi

Creative Commons License

Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού 4.0.

Authors who publish with this journal agree to the following terms:

  1. The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.
  2. Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.
  3. The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a Creative Commons Attribution 4.0 International License or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:
    1. Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;
    with the understanding that the above condition can be waived with permission from the Author and that where the Work or any of its elements is in the public domain under applicable law, that status is in no way affected by the license.
  4. The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.
  5. Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Any such posting made before acceptance and publication of the Work shall be updated upon publication to include a reference to the Publisher-assigned DOI (Digital Object Identifier) and a link to the online abstract for the final published Work in the Journal.
  6. Upon Publisher’s request, the Author agrees to furnish promptly to Publisher, at the Author’s own expense, written evidence of the permissions, licenses, and consents for use of third-party material included within the Work, except as determined by Publisher to be covered by the principles of Fair Use.
  7. The Author represents and warrants that:
    1. the Work is the Author’s original work;
    2. the Author has not transferred, and will not transfer, exclusive rights in the Work to any third party;
    3. the Work is not pending review or under consideration by another publisher;
    4. the Work has not previously been published;
    5. the Work contains no misrepresentation or infringement of the Work or property of other authors or third parties; and
    6. the Work contains no libel, invasion of privacy, or other unlawful matter.
  8. The Author agrees to indemnify and hold Publisher harmless from Author’s breach of the representations and warranties contained in Paragraph 6 above, as well as any claim or proceeding relating to Publisher’s use and publication of any content contained in the Work, including third-party content.

Revised 7/16/2018. Revision Description: Removed outdated link.