Effect of chlorhexidine, honey and Propolis on Streptococcus mutans Counts: in vitro Study

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DOI:

https://doi.org/10.5195/d3000.2021.166

Klíčová slova:

Streptococcus mutans, chlorhexidine, propolis, honey.

Abstrakt

Objective: .Streptococcus mutans is considered one of the bacteria that causes necrosis, as it metabolism sugar to obtain energy, producing an acidic environment and causing depression in the enamel surface, which causes the dissolution of the calcium molecule, a problem of cavited in the tooth; so the aim of the research is to evaluate the effect of mouthwashes chlorhexidine 0.12% - honey 50% - propolis 5% on Streptococcus mutans in children.

Study design: A laboratory study to assess the efficiency of chlorhexidine, honey and Propolis on Streptococcus mutans counts. The sample included 60 children divided into: G1 (Chlorhexidine 0.12% = 20 children), G2 (Propolis 5% = 20 children), G3 (Honey 100% = 20 children). The data was analyzed using the statistical analysis program SPSS, version 13.00, at a confidence level of 95% (P <0.05(. Paired sample T test was used to compare the variable means of the values of the studied variables.

Results: There is significant difference between  three groups. The reduction ratio was 89.97% in G1, 85.49% in G2 and 76.29% in G3.

Conclusion: This study demonstrated the effectiveness of mouthwashes of chlorhexidine, propolis and honey on the bacteria of Streptococcus mutans.

Biografie autora

Maher Al-assaf, Department of Oral Histology and Pathology, Faculty of Dentistry, Damascus University, Damascus, Syria.

Department of Oral Histology and Pathology

Reference

Dental caries is a preventable infectious disease. Balakrishnan M, Simmonds RS, Tagg JR. Aust Dent J. 2000 Dec;45(4):235-245. doi: 10.1111/j.1834-7819.2000.tb00257.x. PMID: 11225524.

Streptococcus mutans, caries and simulation models. Forssten SD, Björklund M, Ouwehand AC. Nutrients. 2010 Mar;2(3):290-298. doi: 10.3390/nu2030290. PMID: 22254021.

Mode of delivery and other maternal factors influence the acquisition of Streptococcus mutans in infants. Li Y, Caufield PW, Dasanayake AP, Wiener HW, Vermund SH. J Dent Res. 2005 Sep;84(9):806-811. doi: 10.1177/154405910508400905. PMID: 16109988.

The effect of caries-preventive measures in mothers on dental caries and the oral presence of the bacteria Streptococcus mutans and lactobacilli in their children. Köhler B, Andréen I, Jonsson B. Arch Oral Biol. 1984 29(11):879-883. doi: 10.1016/0003-9969(84)90086-4. PMID: 6596034.

Bacteria of dental caries in primary and permanent teeth in children and young adults. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ. J Clin Microbiol. 2008 Apr;46(4):1407-1417. doi: 10.1128/JCM.01410-07. PMID: 18216213.

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts. Kadry GM, Ismail MAM, El-Sayed NM, El-Kholy HS, El-Akkad DMH. J Parasit Dis. 2021 Mar;45(1):159-168. doi: 10.1007/s12639-020-01292-8. PMID: 33746401.

Evaluation of new seawater-based mouth rinse versus chlorhexidine 0.2% reducing plaque and gingivitis indexes. a randomized controlled pilot study. Calvo-Guirado JL, Fernandez Dominguez M, Aragoneses JM, Martinez Gonzalez JM, Fernández-Boderau E, Garcés-Villalá MA, et al. Appl. Sci. 2020 Feb;10(3):982. doi: 10.3390/app10030982.

Adjunctive use of chlorhexidine in oral candidoses: a review. Ellepola AN, Samaranayake LP. Oral Dis. 2001 Jan;7(1):11-17. PMID: 11354914.

Management strategies of honey bee diseases. Arbia A, Babbay B. Journal of Entomology. 2011 ;8(1):1-15. doi: 10.3923/je.2011.1.15.

Does propolis help to maintain oral health?. Więckiewicz W, Miernik M, Więckiewicz M, Morawiec T. Evid Based Complement Alternat Med. 2013 jan;2013:351062. doi: 10.1155/2013/351062. PMID: 23365605.

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Barrientos L, Herrera CL, Montenegro G, Ortega X, Veloz J, Alvear M, Cuevas A, Saavedra N, Salazar LA. Braz J Microbiol. 2013 Oct 30;44(2):577-585. doi: 10.1590/S1517-83822013000200038. PMID: 24294257.

Clinical usage of honey as a wound dressing: an update. Molan PC, Betts JA. J Wound Care. 2004 Oct;13(9):353-356. doi: 10.12968/jowc.2004.13.9.26708. PMID: 15517742.

Antibacterial efficacy of raw and processed honey. Mohapatra DP, Thakur V, Brar SK. Biotechnol Res Int. 2011 Dec;2011:917505. doi: 10.4061/2011/917505. PMID: 21350671.

Individual differences in salivary cortisol: associations with common over-the-counter and prescription medication status in infants and their mothers. Hibel LC, Granger DA, Kivlighan KT, Blair C. Horm Behav. 2006 Aug;50(2):293-300. doi: 10.1016/j.yhbeh.2006.03.014. PMID: 16682032.

Number of mutans streptococci or lactobacilli in a total dental plaque sample does not explain the variation in caries better than the numbers in stimulated whole saliva. Sullivan A, Borgström MK, Granath L, Nilsson G. Community Dent Oral Epidemiol. 1996 Jun;24(3):159-163. doi: 10.1111/j.1600-0528.1996.tb00834.x. PMID: 8871012.

Caries risk assessment from dental plaque and salivary Streptococcus mutans counts on two culture media. Sánchez-Pérez L, Acosta-Gío AE. Arch Oral Biol. 2001 Jan;46(1):49-55. doi: 10.1016/s0003-9969(00)00095-9. PMID: 11163595.

Antibacterial activity of selected Malaysian honey. Zainol MI, Mohd Yusoff K, Mohd Yusof MY. BMC Complement Altern Med. 2013 Jun;13:129. doi: 10.1186/1472-6882-13-129. PMID: 23758747.

Evaluation of the effects of manuka honey on salivary levels of mutans streptococci in children: a pilot study. Rupesh S, Winnier JJ, Nayak UA, Rao AP, Reddy NV, Peter J. J Indian Soc Pedod Prev Dent. 2014 Jul-Sep;32(3):212-219. doi: 10.4103/0970-4388.135827. PMID: 25001440.

Effect of honey on Streptococcus mutans growth and biofilm formation. Nassar HM, Li M, Gregory RL. Appl Environ Microbiol. 2012 Jan;78(2):536-540. doi: 10.1128/AEM.05538-11. PMID: 22038612.

Effect of Propolis on Streptococcus mutans Counts: An in vivo Study. Hegde KS, Bhat SS, Rao A, Sain S. Int J Clin Pediatr Dent. 2013 Jan;6(1):22-25. doi: 10.5005/jp-journals-10005-1180. PMID: 25206182.

Comparison of the antimicrobial effect of Egyptian propolis vs New Zealand propolis on Streptococcus mutans and lactobacilli in saliva. Elbaz GA, Elsayad II. Oral Health Prev Dent. 2012;10(2):155-160. doi: 10.3290/j.ohpd.a28003. PMID: 22763595.

Streptococcus mutans and lactobacilli frequency in orthodontic patients; the effect of chlorhexidine treatments. Lundström F, Krasse B. Eur J Orthod. 1987 May;9(2):109-116. doi: 10.1093/ejo/9.2.109. PMID: 3472888.

Stahování

Publikováno

2021-11-19

Číslo

Vol 9 No 1 (2021)

Sekce

Adults & the Elderly

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