Evaluation of Salivary Interleukin 6 and MicroRNA 146a in Patients with Chronic Periodontitis and Their Association with Periodontal Parameters
DOI:
https://doi.org/10.5195/d3000.2025.843Keywords:
chronic periodontitis, salivary IL-6, salivary microRNA146aAbstract
Objective: The aim of this study was to evaluate of the role of interleukin 6 (IL-6) and microRNA 146a (miRNA 146a) in chronic periodontitis (CPD).
Patients and Methods: This cross-sectional case control study was conducted at the Diyala province-Iraq. A total of 40 CPD patients were included. They were 20 males and 20 females, and their age range was 23-56 years. The clinical periodontal parameters were measured under supervision of specialized dentists. Furthermore, 40 apparently healthy individuals, 21 were males and 19 were female, were included as controls. Their age range was 20-50 years. All participants were requested to complete a questionnaire that contained medical and socio-demographic data. The salivary IL-6 was determined using qELISA (INOVA Biotech Co., LTD, China). The salivary miRNA 146a was detected using qPCR (TransGen biotech, China). Statistical analyses were done using SPSS version 22 (Inc., Chicago, Illinois, United States), and P-values ≤ 0.05 were considered significant.
Results: The mean age ± SD of patients was not different than that of controls (38.85 ± 5.30 Vs 40.52 ± 6.69, P=0.2). The gender was also not different (P=0.8). The mean ± SD PI of CPD patients was significantly higher (1.27± 0.34 Vs 0.60 ± 0.26, P=0.01). Similarly, the mean ± SD of GI was also significantly higher in CPD patients (1.49 ± 0.42 Vs 0.24 ± 0.14, P= 0.01). The rate of BOP sites in CPD patients was significantly higher than that of controls (P=0.001). Also, the mean ± SD of PPD in CPD patients was 4.66 ± 0.50 mm, while the mean ± SD of CAL was 4.90 ± 1.30. Additionally, the mean No.± SD of teeth in CPD patients was significantly lower (24.72± 3.77 Vs 27.62± 0.80, P=0.01). Furthermore, the mean No. ± SD of sites in CPD patients was also significantly lower (98.95± 15.08 Vs 110.50 ± 3.22, P 0.01). The mean concentration ± SD of salivary IL-6 was not higher in CPD patients (8.17 ± 3.79 Vs 7.45± 1.51, P= 0.4). qPCR results found that there was significantly higher expression of miRNA-146a in CPD patients versus that of the controls (1.79 ± 0.10 Vs 1.08± 0.10, P=0.001).
Conclusion: The current results concluded that both IL-6 and miRNA 146a were elevated in CPD patients and closely correlated with periodontal parameters suggesting that these biomarkers can be used as a surrogate diagnostic and prognostic indicator of chronic periodontitis.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Suha Khaleel Ibrahim, Ghada Bouslama, Lamia Oualha
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.
- Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.
- The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a Creative Commons Attribution 4.0 International License or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:
- Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;
- The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Any such posting made before acceptance and publication of the Work shall be updated upon publication to include a reference to the Publisher-assigned DOI (Digital Object Identifier) and a link to the online abstract for the final published Work in the Journal.
- Upon Publisher’s request, the Author agrees to furnish promptly to Publisher, at the Author’s own expense, written evidence of the permissions, licenses, and consents for use of third-party material included within the Work, except as determined by Publisher to be covered by the principles of Fair Use.
- The Author represents and warrants that:
- the Work is the Author’s original work;
- the Author has not transferred, and will not transfer, exclusive rights in the Work to any third party;
- the Work is not pending review or under consideration by another publisher;
- the Work has not previously been published;
- the Work contains no misrepresentation or infringement of the Work or property of other authors or third parties; and
- the Work contains no libel, invasion of privacy, or other unlawful matter.
- The Author agrees to indemnify and hold Publisher harmless from Author’s breach of the representations and warranties contained in Paragraph 6 above, as well as any claim or proceeding relating to Publisher’s use and publication of any content contained in the Work, including third-party content.
Revised 7/16/2018. Revision Description: Removed outdated link.
