The Impact of Autoclave Sterilization on Cyclic Fatigue of Nickel-Titanium Rotary Endodontic Instruments

Authors

  • Sura Yaseen Khudhur university of Anbar
  • Ibtihal Mohammed Hussein
  • Ola Mohammed Abdul Kadhum

DOI:

https://doi.org/10.5195/d3000.2025.782

Keywords:

NiTi instruments, Cyclic fatigue, Autoclave

Abstract

Objective: The present study aimed to evaluate the impact of repeating sterilization in an autoclave on the cyclic fatigue resistance for three varieties of nickel-titanium (NiTi) rotary endodontic systems in double curved simulated canal.

Materials and Methods: During this research, three distinct varieties of nickel-titanium rotary endodontic files with a tip diameter of 0.25 millimeters and a taper of 0.06 millimeters were utilized: ProDesign Logic system, E-Flex Edge, and Endostar E3 Azure. Each type of rotary endodontic instruments (n=24) was then randomly divided into three sub-groups: non-sterilized instruments, sterilized instruments after 3 autoclave cycles, and sterilized instruments after 5 autoclave cycles. All the files from the various subgroups were examined to determine their ability to resist cyclic fatigue. Additionally, the period for fractures, the total number of cycles until failure (NCF), and the resulting fracture fragment length for every instrument were determined. SPSS software 29 was utilized for statistical comparisons (P<0.05).

Results: There were no differences among sterilized as well as non-sterilized files (P>0.05). There were no statistically significant differences in fractured length between tested groups.

Conclusions: The resistance to cyclic fatigue of NiTi rotary instruments was not considerably impacted by the repeated autoclave sterilization cycles.

References

Hülsmann, M., O.A. Peters, and P.M. Dummer, Mechanical preparation of root canals: shaping goals, techniques and means. Endodontic topics, 2005. 10(1): p. 30-76.

Knowles, K.I., et al., Incidence of instrument separation using LightSpeed rotary instruments. Journal of endodontics, 2006. 32(1): p. 14-16.

Iqbal, M.K., M.R. Kohli, and J.S. Kim, A retrospective clinical study of incidence of root canal instrument separation in an endodontics graduate program: a PennEndo database study. Journal of endodontics, 2006. 32(11): p. 1048-1052.

Kim, J.-Y., et al., Effect from cyclic fatigue of nickel-titanium rotary files on torsional resistance. Journal of endodontics, 2012. 38(4): p. 527-530.

Parashos, P., I. Gordon, and H.H. Messer, Factors influencing defects of rotary nickel-titanium endodontic instruments after clinical use. Journal of endodontics, 2004. 30(10): p. 722-725.

Shen, Y. and G.S. Cheung, Methods and models to study nickel–titanium instruments. Endodontic topics, 2013. 29(1): p. 18-41.

Zhao, D., et al., Effect of autoclave sterilization on the cyclic fatigue resistance of thermally treated Nickel–Titanium instruments. International Endodontic Journal, 2016. 49(10): p. 990-995.

Champa, C., et al., An analysis of cyclic fatigue resistance of reciprocating instruments in different canal curvatures after immersion in sodium hypochlorite and autoclaving: An in vitro study. Journal of conservative dentistry: JCD, 2017. 20(3): p. 194.

Plotino, G., et al., Experimental evaluation on the influence of autoclave sterilization on the cyclic fatigue of new nickel-titanium rotary instruments. Journal of endodontics, 2012. 38(2): p. 222-225.

Bulem, Ü.K., A.D. Kececi, and H.E. Guldas, Experimental evaluation of cyclic fatigue resistance of four different nickel-titanium instruments after immersion in sodium hypochlorite and/or sterilization. Journal of Applied Oral Science, 2013. 21: p. 505-510.

Shen, Y., et al., Current challenges and concepts of the thermomechanical treatment of nickel-titanium instruments. Journal of endodontics, 2013. 39(2): p. 163-172.

Zinelis, S., et al., The effect of thermal treatment on the resistance of nickel-titanium rotary files in cyclic fatigue. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2007. 103(6): p. 843-847.

Yahata, Y., et al., Effect of heat treatment on transformation temperatures and bending properties of nickel–titanium endodontic instruments. International endodontic journal, 2009. 42(7): p. 621-626.

de Menezes, S.E.A.C., et al., Cyclic fatigue resistance of WaveOne Gold, ProDesign R and ProDesign Logic files in curved canals in vitro. Iranian endodontic journal, 2017. 12(4): p. 468.

Freitas, G.R., et al., Influence of endodontic cavity access on curved root canal preparation with ProDesign Logic rotary instruments. Clinical Oral Investigations, 2021. 25: p. 469-475.

Eighteeth. Changzhou Sifary Medical Technology Co., Ltd. Eighteeth E-Flex Series EndoFile Brochure. 2022.

Azure, E.E. Poldent Co. Ldt. Endostar E3 Azure HT Technology. Instruction for use. 2021.

Alshwaimi, E.O., Effect of sterilization on cyclic fatigue resistance of proflexendo endodontic rotary files. Saudi Journal of Medicine & Medical Sciences, 2019. 7(3): p. 151.

Spagnuolo, G., et al., Effect of autoclaving on the surfaces of TiN‐coated and conventional nickel–titanium rotary instruments. International endodontic journal, 2012. 45(12): p. 1148-1155.

Dioguardi, M., et al., Management of instrument sterilization workflow in endodontics: a systematic review and meta-analysis. International Journal of Dentistry, 2020. 2020.

Alapati, S.B., et al., SEM observations of nickel-titanium rotary endodontic instruments that fractured during clinical use. Journal of endodontics, 2005. 31(1): p. 40-43.

Valois, C.R., L.P. Silva, and R.B. Azevedo, Multiple autoclave cycles affect the surface of rotary nickel-titanium files: an atomic force microscopy study. Journal of Endodontics, 2008. 34(7): p. 859-862.

Hilfer, P.B., et al., Multiple autoclave cycle effects on cyclic fatigue of nickel-titanium rotary files produced by new manufacturing methods. Journal of endodontics, 2011. 37(1): p. 72-74.

Khabiri, M., M. Ebrahimi, and M.R. Saei, The effect of autoclave sterilization on resistance to cyclic fatigue of Hero endodontic file# 642 (6%) at two artificial curvature. Journal of Dentistry, 2017. 18(4): p. 277.

Al-Amidi, A.H. and H.A.-R. Al-Gharrawi, Effect of autoclave sterilization on the cyclic fatigue resistance of EdgeFile X7, 2Shape, and F-one nickel–titanium endodontic instruments. Journal of Conservative Dentistry: JCD, 2023. 26(1): p. 26.

Al-Sudani, D., et al., Cyclic fatigue of nickel-titanium rotary instruments in a double (S-shaped) simulated curvature. Journal of endodontics, 2012. 38(7): p. 987-989.

Yılmaz, K., G. Uslu, and T. Özyürek, In vitro comparison of the cyclic fatigue resistance of HyFlex EDM, One G, and ProGlider nickel titanium glide path instruments in single and double curvature canals. Restorative dentistry & endodontics, 2017. 42(4): p. 282-289.

Mohammed, A.H. and I. Al-zaka, Cyclic fatigue of different glide path systems in single and double curved simulated canal: a comparative study. Int J Med Res Health, 2018. 7(11): p. 72-78.

Plotino, G., et al., Cyclic fatigue of NiTi rotary instruments in a simulated apical abrupt curvature. International Endodontic Journal, 2010. 43(3): p. 226-230.

Bhagabati, N., S. Yadav, and S. Talwar, An in vitro cyclic fatigue analysis of different endodontic nickel-titanium rotary instruments. Journal of endodontics, 2012. 38(4): p. 515-518.

Lopes, H.P., et al., Influence of rotational speed on the cyclic fatigue of rotary nickel-titanium endodontic instruments. Journal of endodontics, 2009. 35(7): p. 1013-1016.

Neelakantan, P., P. Reddy, and J.L. Gutmann, Cyclic fatigue of two different single files with varying kinematics in a simulated double‐curved canal. Journal of Investigative and Clinical Dentistry, 2016. 7(3): p. 272-277.

Hülsmann, M., D. Donnermeyer, and E. Schäfer, A critical appraisal of studies on cyclic fatigue resistance of engine‐driven endodontic instruments. International endodontic journal, 2019. 52(10): p. 1427-1445.

Almohareb, R.A., et al., Effect of autoclaving cycles on the cyclic fatigue resistance of race and race evo nickel-titanium endodontic rotary files: an in vitro study. Metals, 2021. 11(12): p. 1947.

Sharroufna, R. and M. Mashyakhy, The effect of multiple autoclave sterilization on the cyclic fatigue of three heat-treated nickel-titanium rotary files: EdgeFile X7, Vortex Blue, and TRUShape. BioMed Research International, 2020. 2020.

Cho, O.-I., et al., Cyclic fatigue resistance tests of Nickel-Titanium rotary files using simulated canal and weight loading conditions. Restorative dentistry & endodontics, 2013. 38(1): p. 31-35.

Willershausen, B., et al., Radiographic investigation of frequency and location of root canal curvatures in human mandibular anterior incisors in vitro. Journal of endodontics, 2008. 34(2): p. 152-156.

Plotino, G., et al., A review of cyclic fatigue testing of nickel-titanium rotary instruments. Journal of endodontics, 2009. 35(11): p. 1469-1476.

Shim, K.-S., et al., Mechanical and metallurgical properties of various nickel‐titanium rotary instruments. BioMed research international, 2017. 2017(1): p. 4528601.

Bulem, Ü.K., A.D. Kececi, and H.E. Guldas, Experimental evaluation of cyclic fatigue resistance of four different nickel-titanium instruments after immersion in sodium hypochlorite and/or sterilization. Journal of Applied Oral Science, 2013. 21(6): p. 505-510.

Arias, A., et al., Correlation between temperature-dependent fatigue resistance and differential scanning calorimetry analysis for 2 contemporary rotary instruments. Journal of Endodontics, 2018. 44(4): p. 630-634.

Plotino, G., et al., Influence of temperature on cyclic fatigue resistance of ProTaper Gold and ProTaper Universal rotary files. Journal of endodontics, 2017. 43(2): p. 200-202.

Yao, J.H., S.A. Schwartz, and T.J. Beeson, Cyclic fatigue of three types of rotary nickel-titanium files in a dynamic model. Journal of endodontics, 2006. 32(1): p. 55-57.

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Published

2025-02-12

Issue

Vol. 13 No. 1 (2025)

Section

Adults & the Elderly

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Copyright (c) 2025 Sura Yaseen Khudhur, Ibtihal Mohammed Hussein, Ola Mohammed Abdul Kadhum

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