Impact of Clove and Eucalyptus Essential Oils versus Fluoride on Resin Hardness and Selected Oral Microorganisms
DOI:
https://doi.org/10.5195/d3000.2026.1594Keywords:
Enamel, Resin, Oils, HardnessAbstract
The objectives of this study were to assess the effect of syzygium aromaticum (clove) and Eucalyptus essential oils on hardness of acrylic resin and selected oral pathogens (Streptococcus mutans, Staphylococcus aureus and Candida albicans) in comparison to calcium fluoride. 120 samples were constructed from heat cured material then divided into 2 groups, for microbial test and for hardness test, then these samples subdivided according to additive incorporation: control without additive, 2.5% eucalyptus oil, 2.5% syzygium aromaticum oil and calcium fluoride (CaF2) with concentration (0.5%, 1.5% and 2.5%). Surface hardness test (measured via shore D tester), Agar well technique for assessing sensitivity test and ELISA reader to confirm adherence of Streptococcus mutans, Staphylococcus aureus and Candida albicans. Hardness mean value were increased significantly in syzygium aromaticum and eucalyptus oil groups in comparison to control group, all CaF2 groups showed no significant differences with control. All tested groups revealed weak adherence against Streptococcus mutans, Staphylococcus aureus and Candida albicans. High inhibition zone value recorded by eucalyptus oil. While syzygium aromaticum oil and CaF2 groups recorded no inhibition zone. Incorporation of (2.5%) for syzygium aromaticum and eucalyptus oil extracts were significantly improved hardness of heat cured resin material, while 0.5%, 1.5% and 2.5%CaF2 showed no significant effect on hardness, both oil extracts and CaF2 demonstrated antimicrobial and revealed weak adherence effect against Streptococcus mutans, Staphylococcus aureus and Candida albicans.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Amal Abdul Latif Rashid, Jinan Mohammed Rashad Shihab, Fatima Kadhim Ghadeer

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.
- Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.
- The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a Creative Commons Attribution 4.0 International License or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:
- Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;
- The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Any such posting made before acceptance and publication of the Work shall be updated upon publication to include a reference to the Publisher-assigned DOI (Digital Object Identifier) and a link to the online abstract for the final published Work in the Journal.
- Upon Publisher’s request, the Author agrees to furnish promptly to Publisher, at the Author’s own expense, written evidence of the permissions, licenses, and consents for use of third-party material included within the Work, except as determined by Publisher to be covered by the principles of Fair Use.
- The Author represents and warrants that:
- the Work is the Author’s original work;
- the Author has not transferred, and will not transfer, exclusive rights in the Work to any third party;
- the Work is not pending review or under consideration by another publisher;
- the Work has not previously been published;
- the Work contains no misrepresentation or infringement of the Work or property of other authors or third parties; and
- the Work contains no libel, invasion of privacy, or other unlawful matter.
- The Author agrees to indemnify and hold Publisher harmless from Author’s breach of the representations and warranties contained in Paragraph 6 above, as well as any claim or proceeding relating to Publisher’s use and publication of any content contained in the Work, including third-party content.
Revised 7/16/2018. Revision Description: Removed outdated link.
