Serum C-Reactive Protein as a Biomarker of Periodontal Disease

A Comparative Study of ELISA and Latex Agglutination

Authors

  • Jehan Yawez Al-Demirchi Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq
  • Hussein Bassim Al-Anssari

DOI:

https://doi.org/10.5195/d3000.2026.1511

Keywords:

Periodontitis, Inflammation, Tooth Loss

Abstract

This study aimed to compare two laboratory methods, enzyle-linked immunosorbent assay (ELISA) and the latex agglutination test, for the detection of serum C-reactive protein in individuals with different periodontal health statuses. Inn addition, the study evaluated the association between CRP levels, periodontal disease severity, age, and gender. A case-control study including 90 participants (30 healthy, 30 with gingivitis, and 30 with periodontitis was performed. Clinical periodontal examinations were done, and serum samples were collected for CRP analysis by latex agglutionation rtest and ELISA. Chi-square, one-way ANOVA, and Tukey post hoc analyses were performed to compare CRP levels among the study groups and to determine correlations between CRP levels, disease severity, age, and gender. Serum CRP levels increased significantly with periodontal disease severity (P < 0.001). ELISA detected higher CRP concentrations from healthy controls than from individuals with periodontitis, while the latex agglutination test showed high specificity (96.2%) but low sensitivity (27%) for CRP detection. The elevation was associated with increasing disease severity, indicating its potential use as a biomarker of periodontal inflammation. Age showed a significant association with disease severity, whereas geneder was not significantly associated. Serum CRP levels increased significantly with periodontal disease severity. Although the latex agglutination test demonstated high specificity abd ay serve as a rapid screenkng method, ELISA provided superior sensitivity and quantitative accuracy, making it the preferred method for clinical and research applications.

Downloads

Published

2026-08-14

Issue

Vol. 14 No. 2 (2026)

Section

Adults & the Elderly

License

Copyright (c) 2026 Jehan Yawez Al-Demirchi, Hussein Bassim Al-Anssari

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.
  2. Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.
  3. The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a Creative Commons Attribution 4.0 International License or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:
    1. Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;
    with the understanding that the above condition can be waived with permission from the Author and that where the Work or any of its elements is in the public domain under applicable law, that status is in no way affected by the license.
  4. The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.
  5. Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Any such posting made before acceptance and publication of the Work shall be updated upon publication to include a reference to the Publisher-assigned DOI (Digital Object Identifier) and a link to the online abstract for the final published Work in the Journal.
  6. Upon Publisher’s request, the Author agrees to furnish promptly to Publisher, at the Author’s own expense, written evidence of the permissions, licenses, and consents for use of third-party material included within the Work, except as determined by Publisher to be covered by the principles of Fair Use.
  7. The Author represents and warrants that:
    1. the Work is the Author’s original work;
    2. the Author has not transferred, and will not transfer, exclusive rights in the Work to any third party;
    3. the Work is not pending review or under consideration by another publisher;
    4. the Work has not previously been published;
    5. the Work contains no misrepresentation or infringement of the Work or property of other authors or third parties; and
    6. the Work contains no libel, invasion of privacy, or other unlawful matter.
  8. The Author agrees to indemnify and hold Publisher harmless from Author’s breach of the representations and warranties contained in Paragraph 6 above, as well as any claim or proceeding relating to Publisher’s use and publication of any content contained in the Work, including third-party content.

Revised 7/16/2018. Revision Description: Removed outdated link.