In-Vitro antibacterial activity of glass ionomer cements containing silver nanoparticles synthesized from leaf extract of Mentha piperita

Authors

  • Mahdjoube Goldani Moghadam Assistant professor, Department of Orthodontics, Faculty of Dentistry, Birjand University of Medical Sciences, Birjand, Iran.
  • Aylin Bagherzade MD Dentist, Faculty of Dentistry, Birjand University of Medical Sciences, Birjand, Iran.
  • Fateme Ghorbanzade MD Dentist, Faculty of Dentistry, Birjand University of Medical Sciences, Birjand, Iran.
  • Mohammad Yahya Hanafi-Bojd Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran. Nanomedicine Department, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
  • Masoud Yousefi Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.

DOI:

https://doi.org/10.5195/d3000.2022.267

Keywords:

Antibacterial activity, Glass ionomer cement, Mentha piperita, Silver nanoparticles

Abstract

Purpose: The aim of this study was to evaluate the antibacterial activity of glass ionomer cement incorporated with silver nanoparticles (AgNPs) synthetized using mint leaf extract (Mentha piperita, M. piperita) on some oral cavity bacteria.

Materials and methods: In the present study, M. piperita leaf extract was used for the synthesis of AgNPs. A total of 60 glass ionomer cement (GIC) disk-shaped specimens were prepared and divided into two groups: conventional GIC (C-GIC), and glass ionomer cement with 2 wt% AgNPs (GIC-AGNPs). The antibacterial activity of the GIC specimens in comparison with Ampicillin disk (10 µg/ml) was investigated against Streptococcus mutans, Enterococcus faecalis, Lactobacillus acidophilus, Lactobacillus casei, and Streptococcus aureus by measuring the diameter of growth inhibition zones.

Results: C-GIC specimens failed to show any antibacterial effect against the studied bacteria. However, the GIC-AgNPs had relatively significant antibacterial effects on S. mutans, L. acidophilus, L. casei and S. aureus. The highest antibacterial effect of GIC-AgNPs specimens was reported against L. acidophilus (P <0.001). GIC-AgNPs had no antibacterial effect on E. faecalis.

Conclusion: Glass ionomer cement incorporated with AgNPs synthetized using M. piperita showed a promising antibacterial effect against oral cariogenic pathogens.

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Published

2022-07-13

Issue

Vol. 10 No. 1 (2022)

Section

Mechanisms of Oral Disease

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