The influence of IGF-I and Emdogain on the behavior of dental epithelial cells in a three-dimensional scaffold model

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  • Maria Johanna Jacoba Heezen Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Dentistry – Regenerative Biomaterials, Philips van Leydenlaan 25, 6525EX Nijmegen, the Netherlands
  • Weibo Zhang Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America
  • Sara Rudolph Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America
  • X. Frank Walboomers Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Dentistry – Regenerative Biomaterials, Philips van Leydenlaan 25, 6525EX Nijmegen, the Netherlands
  • Pamela C. Yelick Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America

DOI:

https://doi.org/10.5195/d3000.2022.258

Klíčová slova:

Tissue engineering, Dental epithelial/mesenchymal cells, scaffold, IGF-1, Emdogain

Abstrakt

Aim: Tooth decay and associated periodontal disease remain the most common chronic diseases in current society. In the future, individually tailored, more effective therapeutic treatment options could be provided through the application of tissue engineering and regenerative medicine and dentistry (TERMD) approaches. A relevant finding is the potential of both dental mesenchymal and dental epithelial cells to regenerate mineralized dentin and enamel tissues, respectively.

Materials and Methods: In the current study, a multi-layered, bioengineered tooth bud model was assembled by combining human dental mesenchymal (hDM) and porcine dental epithelial cells (pDE). The hDM cells were seeded onto poly(epsilon-caprolactone)/ poly (lactide-co-glycolide) (PCL/PLGA) wet electro-spun scaffolds overlayed with confluent pDE cell sheet harvested from thermo-reversible tissue culture plates. The so-formed multi-layered bioengineered tooth bud was then used to study the mineralization potential of the dental cells in in vitro culture. It was hypothesized that the addition of the in media soluble factors Insulin-like growth factor 1 (IGF-I) and the extracellular matrix derivate Emdogain (EMD) would result in enhanced differentiation and mineralized dental tissue formation. Scanning Electron morphological observation was used to characterize scaffolds porosity. Histological and immunofluorescent analyses confirmed the localization of hDM cells inside the scaffold, an intact pDE cell sheet, and the presence of beta-integrin 1-positive cell-cell junctions connecting the two.

Results: Scanning Electron Microscopy showed that EMD, in particular, enhanced the mineralization potential of pDE cells. qRT-PCR analyses showed that both EMD and IGF-1 significantly enhanced the expression of Ameloblastin (AMBN), reflecting pDE cell differentiation.

Conclusion: In conclusion, these results proved the hypothesis that both EMD and IGF-1 should be considered for their utility in preclinical dental tissue engineering approaches.

Biografie autora

Maria Johanna Jacoba Heezen, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Dentistry – Regenerative Biomaterials, Philips van Leydenlaan 25, 6525EX Nijmegen, the Netherlands

Master of Science in Dentistry

Weibo Zhang, Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America

Assistant professor

Weibo Zhang, DDS, MS, MDS, PhD

Sara Rudolph, Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America

Bachelor of Science

X. Frank Walboomers, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Dentistry – Regenerative Biomaterials, Philips van Leydenlaan 25, 6525EX Nijmegen, the Netherlands

Dr. X. Frank Walboomers

Associate Professor Regenerative Biomaterials

Head Research Lab Dentistry

Pamela C. Yelick, Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, United States of America

Pamela C. Yelick, Ph.D.

Professor, Department of Orthodontics

Director, Division of Craniofacial and Molecular Genetics

Director, Genetics Program, Tufts Graduate School of Biomedical Sciences

Reference

Kassebaum NJ, Smith AGC, Bernabe E, Fleming TD, Reynolds AE, Vos T, et al. Global, Regional, and National Prevalence, Incidence, and Disability-Adjusted Life Years for Oral Conditions for 195 Countries, 1990-2015: A Systematic Analysis for the Global Burden of Diseases, Injuries, and Risk Factors. J Dent Res. 2017;96(4):380-7.

Hendrik Meyer-Leuckel SP, Kim R. Ekstrand. Caries Management - Science and Clinical practice. In: Effenberger DS, editor. Stuttgart New York: Thieme; 2013. p. 8,9.

Zarb GA. The replacement of missing teeth. Canadian family physician Medecin de famille canadien. 1988;34:1435-40.

Cai X, Ten Hoopen S, Zhang W, Yi C, Yang W, Yang F, et al. Influence of highly porous electrospun PLGA/PCL/nHA fibrous scaffolds on the differentiation of tooth bud cells in vitro. Journal of biomedical materials research Part A. 2017;105(9):2597-607.

Boudriot U, Dersch R, Greiner A, Wendorff JH. Electrospinning approaches toward scaffold engineering--a brief overview. Artificial organs. 2006;30(10):785-92.

van Manen EH, Zhang W, Walboomers XF, Vazquez B, Yang F, Ji W, et al. The influence of electrospun fibre scaffold orientation and nano-hydroxyapatite content on the development of tooth bud stem cells in vitro. Odontology. 2014;102(1):14-21.

Yen AH, Yelick PC. Dental tissue regeneration - a mini-review. Gerontology. 2011;57(1):85-94.

Monteiro N, Yelick PC. Advances and perspectives in tooth tissue engineering. Journal of tissue engineering and regenerative medicine. 2017;11(9):2443-61.

Straumann.com [Available from: https://www.straumann.com/en/dental-professionals/products-and-solutions/biomaterials/biologics.html.

Kobayashi E, Fujioka-Kobayashi M, Saulacic N, Schaller B, Sculean A, Miron RJ. Effect of enamel matrix derivative liquid in combination with a natural bone mineral on new bone formation in a rabbit GBR model. Clin Oral Implants Res. 2019;30(6):542-9.

Dahake PT, Panpaliya NP, Kale YJ, Dadpe MV, Kendre SB, Bogar C. Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials - An in vitro experimental study. Saudi Dent J. 2020;32(1):43-51.

Joseph BK, Savage NW, Young WG, Gupta GS, Breier BH, Waters MJ. Expression and regulation of insulin-like growth factor-I in the rat incisor. Growth Factors. 1993;8(4):267-75.

Kouda K, Iki M, Ohara K, Nakamura H, Fujita Y, Nishiyama T. Associations between serum levels of insulin-like growth factor-I and bone mineral acquisition in pubertal children: a 3-year follow-up study in Hamamatsu, Japan. J Physiol Anthropol. 2019;38(1):16.

Monteiro N, Smith EE, Angstadt S, Zhang W, Khademhosseini A, Yelick PC. Dental cell sheet biomimetic tooth bud model. Biomaterials. 2016;106:167-79.

Scientific TF. [Available from: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/D17402.pdf.

Buizer AT, Veldhuizen AG, Bulstra SK, Kuijer R. Static versus vacuum cell seeding on high and low porosity ceramic scaffolds. Journal of biomaterials applications. 2014;29(1):3-13.

Seo JB, Moody M, Koh DS. Epithelial monolayer culture system for real-time single-cell analyses. Physiological reports. 2014;2(4):e12002.

Grandin HM, Gemperli AC, Dard M. Enamel matrix derivative: a review of cellular effects in vitro and a model of molecular arrangement and functioning. Tissue Eng Part B Rev. 2012;18(3):181-202.

Qu Z, Laky M, Ulm C, Matejka M, Dard M, Andrukhov O, et al. Effect of Emdogain on proliferation and migration of different periodontal tissue-associated cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109(6):924-31.

Li X, Liao D, Sun G, Chu H. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog. J Cell Mol Med. 2020;24(9):5146-51.

Stahování

Publikováno

2022-07-08

Číslo

Sekce

Development of Craniofacial Structures