Dentistry 3000 https://dentistry3000.pitt.edu/ojs/dentistry3000 Dentistry 3000 is a free online dental journal en-US <p>Authors who publish with this journal agree to the following terms:</p><ol><li>The Author retains copyright in the Work, where the term “Work” shall include all digital objects that may result in subsequent electronic publication or distribution.</li><li>Upon acceptance of the Work, the author shall grant to the Publisher the right of first publication of the Work.</li><li>The Author shall grant to the Publisher and its agents the nonexclusive perpetual right and license to publish, archive, and make accessible the Work in whole or in part in all forms of media now or hereafter known under a <a title="CC-BY" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a> or its equivalent, which, for the avoidance of doubt, allows others to copy, distribute, and transmit the Work under the following conditions:<ol type="a"><li>Attribution—other users must attribute the Work in the manner specified by the author as indicated on the journal Web site;</li></ol>with the understanding that the above condition can be waived with permission from the Author and that where the Work or any of its elements is in the public domain under applicable law, that status is in no way affected by the license.</li><li>The Author is able to enter into separate, additional contractual arrangements for the nonexclusive distribution of the journal's published version of the Work (e.g., post it to an institutional repository or publish it in a book), as long as there is provided in the document an acknowledgement of its initial publication in this journal.</li><li>Authors are permitted and encouraged to post online a prepublication manuscript (but not the Publisher’s final formatted PDF version of the Work) in institutional repositories or on their Websites prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. 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Revision Description: Removed outdated link. </span></p> dentistry3000@mail.pitt.edu (Alexandre R. Vieira) dentistry3000@mail.pitt.edu (Technical Support) Wed, 15 Jan 2025 08:56:23 -0500 OJS 3.3.0.13 http://blogs.law.harvard.edu/tech/rss 60 Impact of Soft Drinks Consumption on Salivary pH Levels https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/803 <div> <div> <p class="AbstractText"><strong>Objective: </strong>The goal of current investigation was to determine the impact of some drinks on saliva pH values.</p> <p class="AbstractText"><strong>Methodology: </strong>The study included 20 healthy individuals (15 females and 5 males) with different ages. Measurements after 0, 10, 20, 30, 40, 50, and 60 minutes of drinking Cola, Pepsi, Seven Up, Shani, and Mirinda on saliva pH were determined.</p> <p class="AbstractText"><strong>Results: </strong>Very similar pH value changes happened for all drinks, with a decrease in pH within 10 minutes after drinking that return to original values after 60 minutes.</p> <p class="AbstractText"><strong>Conclusion: </strong>The studied drinks cause a decrease in salivary pH that returns to original values within 60 minutes.</p> </div> </div> Doaa Alghaffari, Yaseen H. Motea, Hiba Alogaidi Copyright (c) 2025 Doaa Alghaffari, Yaseen H. Motea, Hiba Alogaidi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/803 Thu, 13 Feb 2025 00:00:00 -0500 Clinical Evaluation of Patient Satisfaction with Aesthetics, Retention, Function, and Comfort of Removable Complete and Partial Acrylic Dentures https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/832 <p><strong>Objectives: </strong>The quality of removable dentures (acrylic partial and complete) significantly affects patients’ quality of life, encompassing aspects such as aesthetics, function and the psychological well-being of individuals by restoring their smile. This study aimed to evaluate patients’ satisfaction with aesthetics, support, function, and comfort of acrylic removable partial and complete dentures and their acceptance of the procedure.</p> <p><strong>Materials and Methods:</strong> A total of 146 randomly selected partially and completely edentulous patients (106 males and 40 females) with ages 30 years or more were included in this study. All participating patients (120 partially, 23 completely edentulous and 3 patients having one completely edentulous arch and another one is partially edentulous) were informed about the objectives of the study to get their acceptance in terms of esthetic, function, retention, comfort and some other matters by answering some questions (a questionnaire consists of 17 multiple choice questions, using Likert-type scale).</p> <p><strong>Results: </strong>There was an acceptable level of patient's satisfaction with their constructed removable dentures, but they were more satisfied with their removable partial denture in comparison to their complete one regarding the retention of the upper denture (p=0.007), chewing ability (p=0.025), speech (p=0.005) and comfort (p=0.003). Lower denture retention (p=0.062), aesthetic patient's appearance, (p=0.122), getting ulcers after wearing the denture (p=0.125) and difficulties during denture construction visits (p=0.838) did not show the same differences. Most patients (68.49%) had been wearing dentures for more than a year.</p> <p><strong>Conclusions: </strong>This study found generally positive patient experiences with both removable partial and complete dentures, with higher satisfaction observed among those with partial dentures, particularly regarding retention, chewing, speech, and comfort. These findings highlight the benefits of preserving natural teeth and the importance of individualized care, especially during the initial adjustment period, to optimize long-term denture success.</p> Amal Qasim Ahmed, Rusul Subhi Hassan, Hussein Ali M. Hussein, Amenah Hafedh Khudhair, Nabaa Basim Alhusseini, Salah M. Ibrahim Copyright (c) 2025 Amal Qasim Ahmed, Rusul Subhi Hassan, Hussein Ali M. Hussein, Amenah Hafedh Khudhair, Nabaa Basim Alhusseini, Salah M. Ibrahim https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/832 Wed, 23 Apr 2025 00:00:00 -0400 Comparison of Adhesion of Soft-Liner between Conventional PMMA and 3D Printed Resin Denture Bases https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/785 <p><strong>Aims: </strong>The purpose of this study was to evaluate the tensile strength of the soft-liner (Molloplast-B®) between the denture foundation made of 3D printed resin and traditional acrylic (PMMA).</p> <p><strong>Materials and Methods</strong>: In this experiment, 60 acrylic specimens in the shape of reverse dumbbells were employed. The first group consisted of 24 specimens made from traditional acrylic polymethyl methacrylate (PMMA) (Vertex, Veracril, and Duradent) while the second group consisted of 24 specimens made from 3D printed resin (Dentona). The third group consisted of 12 samples that were packed at the dough stage of acrylic and soft liner at the same time.</p> <p>After that, the samples from the first and second groups were split in two, with one half receiving no surface treatment and the other half undergoing sandblasting. Every sample is separated into two halves with precise measurements. The thickest and thinnest sections were 80 mm in length (10 mm x 25 mm) and 8 mm x 15 mm, respectively, and were joined in the middle by the soft-liner material. The experiment's tensile strength was examined in two separate directions to ascertain the soft-liner material's strength of adhesion to the sample's chosen materials.</p> <p><strong>Results</strong>: The data analysis of the tensile strength test revealed a statistically significant difference in the soft-liner adhesion strength between the 3D-printed acrylic resin with sandblast treatment (0.2133 ± 0.03939 kN/mm²) and the 3D-printed acrylic resin without surface treatment (0.1567 ± 0.04677 kN/mm²). Similar results were observed for conventional acrylic PMMA, where the sandblasted acrylic PMMA (0.0950 ± 0.03606 kN/mm²) exhibited significantly better bond strength compared to the untreated acrylic PMMA (0.0875 ± 0.02491 kN/mm²). Dough stage samples had better values complared to traditional acrylic (0.1483± 0.03689 kN/mm²).</p> <p><strong>Conclusion</strong>: The results demonstrate that sandblast treatment significantly improves the soft-liner adhesion strength of both 3D-printed acrylic resin and conventional acrylic PMMA. Surface treatment enhances bond strength compared to untreated materials. These findings suggest that sandblasting is an effective method for improving adhesion in acrylic-based materials.</p> Dawood Jadaan, Juzailah Roffie, Husniyati Roslan, Nahas P Copyright (c) 2025 Dawood Jadaan, Juzailah Roffie, Husniyati Roslan, Nahas P https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/785 Thu, 06 Feb 2025 00:00:00 -0500 Selecting the Best Measuring Device to Evaluate the Dimensional Changes in Complete Denture Samples https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/829 <div> <div> <p class="AbstractText"><strong>Objectives: </strong>Evaluating the accuracy of measuring tools used to access dimensional stability of complete denture samples.</p> <p class="AbstractText"><strong>Materials and Methods: </strong>Ten maxillary complete denture bases were made from heat-cure acrylic resin. Six reference points were prepared in each sample in the central incisors area, first premolars area, and first molars area. The distances between 5 different locations (3 horizontal distances and 2 anteroposterior distances) were measured using 3 different measuring tools. Each sample was measured three times using measuring microscope, sagittal cephalometric radiograph in conjunction with auto CAD soft-ware, and Vernier caliper. The results were analyzed using One-way ANOVA and post Tukey test.</p> <p class="AbstractText"><strong>Results: </strong>the type of measuring tool significantly affect the measurements especially with the radiographs that showed larger values compared to the microscope and Vernier caliper (P&lt;0.05). The latter two measuring tools were equivalent to each other (P &gt;0.05).</p> <p class="AbstractText"><strong>Conclusions: </strong>within the limitations of the present study, the measuring microscope is the best tool to be used for measuring distances as it provides the best visibility and validity. Vernier calipers is a better alternative for the microscope compare to the radiographs and computer software.</p> </div> </div> Sanaa Rasheed Abd Al-Aaloosi, Ghasaq Abdullah Mohmood, Mustafa Sameer Mahmood Al-Shaikhli Copyright (c) 2025 Sanaa Rasheed Abd Al-Aaloosi, Ghasaq Abdullah Mohmood, Mustafa Sameer Mahmood Al-Shaikhli https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/829 Fri, 28 Mar 2025 00:00:00 -0400 The Impact of Autoclave Sterilization on Cyclic Fatigue of Nickel-Titanium Rotary Endodontic Instruments https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/782 <div> <div> <p class="AbstractText"><strong>Objective</strong>: The present study aimed to evaluate the impact of repeating sterilization in an autoclave on the cyclic fatigue resistance for three varieties of nickel-titanium (NiTi) rotary endodontic systems in double curved simulated canal.</p> <p class="AbstractText"><strong>Materials and Methods</strong>: During this research, three distinct varieties of nickel-titanium rotary endodontic files with a tip diameter of 0.25 millimeters and a taper of 0.06 millimeters were utilized: ProDesign Logic system, E-Flex Edge, and Endostar E3 Azure. Each type of rotary endodontic instruments (n=24) was then randomly divided into three sub-groups: non-sterilized instruments, sterilized instruments after 3 autoclave cycles, and sterilized instruments after 5 autoclave cycles. All the files from the various subgroups were examined to determine their ability to resist cyclic fatigue. Additionally, the period for fractures, the total number of cycles until failure (NCF), and the resulting fracture fragment length for every instrument were determined. SPSS software 29 was utilized for statistical comparisons (P&lt;0.05).</p> <p class="AbstractText"><strong>Results</strong>: There were no differences among sterilized as well as non-sterilized files (P&gt;0.05). There were no statistically significant differences in fractured length between tested groups.</p> <p class="AbstractText"><strong>Conclusions</strong>: The resistance to cyclic fatigue of NiTi rotary instruments was not considerably impacted by the repeated autoclave sterilization cycles.</p> </div> </div> Sura Yaseen Khudhur, Ibtihal Mohammed Hussein, Ola Mohammed Abdul Kadhum Copyright (c) 2025 Sura Yaseen Khudhur, Ibtihal Mohammed Hussein, Ola Mohammed Abdul Kadhum https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/782 Wed, 12 Feb 2025 00:00:00 -0500 The Impact of Thermocycling on the Physico-mechanical Properties of SLA and DLP 3D-Printed Dental Ceramics: A Comparative Study https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/866 <p><strong>Objective</strong>: Three-dimensional (3D) printing technology is highly promising for producing nanoceramic resin dental restorations. However, the effects of environmental stressors on the structural integrity and clinical performance of these restorations require further elucidation. To investigate the effects of Stereolithography (SLA) and digital light processing (DLP) 3D printing technologies on the physical-mechanical properties of a 3D-printed resin material used in dental applications.</p> <p><strong>Methods</strong>: A total of 120 resin specimens (Senertek P-Crown V2) were fabricated using SLA and DLP technologies. The microhardness, flexural strength, and surface roughness of the specimens were evaluated under control and thermocycling conditions to evaluate their long-term performance. T<span style="font-size: 0.875rem;">o assess statistical significance a two independent sample t-tests (</span><em style="font-size: 0.875rem;">P </em><em style="font-size: 0.875rem;">&lt;</em><em style="font-size: 0.875rem;"> 0.05</em><span style="font-size: 0.875rem;">) were used to analysis the data.</span></p> <p><strong>Results</strong>: SLA samples exhibited significantly higher microhardness (<em>P = 0.001</em>) and flexural strength than DLP samples, both in the control state and after thermocycling. After thermocycling, the microhardness of SLA samples increased, whereas that of DLP samples decreased. Surface roughness values increased significantly in both SLA and DLP samples after thermocycling, with SLA samples exhibiting higher roughness values.</p> <p><strong>Conclusion</strong>: SLA-printed resin demonstrated superior microhardness and flexural strength compared to DLP-printed resin. However, its long-term durability is affected by immersion and thermocycling. This study highlights the impact of water sorption, polymerization mechanisms, and surface morphology on material performance.</p> Nadia H Hasan Copyright (c) 2025 Nadia H Hasan https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/866 Mon, 21 Apr 2025 00:00:00 -0400 Evaluation of the Impact of Nanoparticle Additives on the Shear Bond Strength between Soft Liners and Acrylic https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/848 <div> <div> <p class="AbstractText"><strong>Objectives: </strong>The study aimed to evaluate the antifungal properties of incorporating two types of nanoparticles into the denture soft lining material, and shear bond strength between the reinforced soft liner and acrylic resin.</p> <p class="AbstractText"><strong>Materials and Methods: </strong>Ninety specimens were fabricated and grouped into three distinct groups named group A which is the control, group B involved cerium oxide nanoparticles infused soft liner, and group C involved zinc oxide nanoparticles infused soft liner specimens. Specimens for shear bond strength consist of two acrylic parts and one soft liner part occupying the space between the acrylic specimens. Antifungal properties were tested by counting the number of viable cells visually, and shear bond strength was tested using a universal testing machine at speed of 0.5 mm per minute. Statistical tests involved one-way ANOVA and post hoc tests at P ≤ 0.05.</p> <p class="AbstractText"><strong>Results: </strong>Cerium oxide nanoparticles infused specimens revealed higher antifungal properties compared to zinc oxide infused specimens and control specimens. Also, higher shear bond strengths (0.419) followed by those infused with zinc oxide nanoparticles (0.342), while the lowest values were for the control group (0.305).</p> </div> </div> <div><strong>Conclusions: </strong>The observed enhancements in antifungal and mechanical properties resulting from cerium oxide and zinc oxide nanoparticles infusions underscore their considerable potential as effective additives for improving performance in various applications.</div> Nusaiba Mustafa Muhammed Copyright (c) 2025 Nusaiba Mustafa Muhammed https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/848 Fri, 18 Apr 2025 00:00:00 -0400 Periodontal and Biochemical Correlations during Alendronate Treatment in Postmenopausal Women: A Longitudinal Study https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/813 <div> <div> <p class="AbstractText"><strong>Objective</strong>: Postmenopausal osteoporosis (OP) is a prevalent condition often treated with bisphosphonates like alendronate, which may influence periodontal health. In this study, we aimed to evaluate the relationship between clinical periodontal parameters [probing pocket depth (PPD), clinical attachment level (CAL)], biochemical markers [osteoprotegerin (OPG), and calcium (Ca)] and alendronate therapy.</p> <p class="AbstractText"><strong>Methods</strong>: This case-control study was carried out on 60 females aged from 55 to 65. All participants were divided into two groups equally, group A that included healthy postmenopausal females with healthy periodontium and group B included patients with osteoporosis. Patients were followed up for one year through for three consecutive periods (0, 6, and 12 months) after receiving alendronate (ALN) treatment. Patients with osteoporosis or ALN were further subdivided equally based on gingivitis and periodontitis. Blood samples were gathered for quantitative proportions of OPG through enzyme–linked immune sorbent assay (ELISA). Additionally, calcium was analyzed using full automated calcium detector device. The clinical periodontal parameters (PPD, and CAL) were reported for all teeth except for third molars and kept in specifically designed case sheet documents following assortment of serum.</p> <p class="AbstractText"><strong>Results</strong>: There was a significant decrease in PPD and CAL after 12 months of ALN treatment. However, there was significant increase in serum OPG and calcium level at each consecutive recall follow up in comparison to no ALN treatment. Additionally, our study revealed a positive correlation between OPG and calcium at base line in patients with osteoporosis (p&lt;0.001).</p> <p class="AbstractText"><strong>Conclusion</strong>: Probing pocket depth, clinical attachment level and osteoprotegerin and calcium serum levels improved significantly with alendronate therapy.</p> </div> </div> Aseel Jalil Ibrahim Al-Karawi, Hajer Ali Ahmed Hentati Copyright (c) 2025 Aseel Jalil Ibrahim Al-Karawi, Hajer Ali Ahmed Hentati https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/813 Wed, 12 Feb 2025 00:00:00 -0500 Potential Role of Periodontopathogens in Rheumatoid Arthritis https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/808 <div> <div> <p class="AbstractText">Periodontal disease, particularly the chronic form of periodontitis, is a widespread inflammatory condition caused by pathogenic bacteria, such as <em>Porphyromonas gingivalis</em>. This review explores the potential role of periodontopathogens, particularly <em>Porphyromonas gingivalis</em>, in the development and progression of rheumatoid arthritis. Rheumatoid arthritis is a systemic autoimmune disorder characterized by joint inflammation and damage. Both periodontitis and rheumatoid arthritis share common inflammatory mechanisms, driven by proinflammatory cytokines and immune dysregulation. <em>Porphyromonas gingivalis</em>, via its pathogenic determinants, that is, gingipains and peptidyl arginine deiminase, facilitates the citrullination of proteins, leading to the generation of anti-citrullinated protein antibodies, a key feature in rheumatoid arthritis pathogenesis. The reciprocal relationship between periodontitis and rheumatoid arthritis is further supported through evidence showing that individuals with periodontitis are at a higher risk for rheumatoid arthritis, and vice versa. Both circumstances exhibit similar inflammatory pathways, involving cytokines like tumor necrosis factor alpha, interleukin 1 and interleukin 6 and tissue degradation mediated by matrix metalloproteinases.</p> </div> </div> Shaymaa Abdulkareem Nori, Batool Hassan Al-Ghurabi Copyright (c) 2025 Shaymaa Abdulkareem Nori, Batool Hassan Al-Ghurabi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/808 Thu, 13 Feb 2025 00:00:00 -0500 Evaluation of Salivary Interleukin 6 and MicroRNA 146a in Patients with Chronic Periodontitis and Their Association with Periodontal Parameters https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/843 <p><strong>Objective: </strong>The aim of this study was to evaluate of the role of interleukin 6 (IL-6) and microRNA 146a (miRNA 146a) in chronic periodontitis (CPD).</p> <p><strong>Patients and Methods: </strong>This cross-sectional case control study was conducted at the Diyala province-Iraq. A total of 40 CPD patients were included. They were 20 males and 20 females, and their age range was 23-56 years. The clinical periodontal parameters were measured under supervision of specialized dentists. Furthermore, 40 apparently healthy individuals, 21 were males and 19 were female, were included as controls. Their age range was 20-50 years. All participants were requested to complete a questionnaire that contained medical and socio-demographic data. The salivary IL-6 was determined using qELISA (INOVA Biotech Co., LTD, China). The salivary miRNA 146a was detected using qPCR (TransGen biotech, China). Statistical analyses were done using SPSS version 22 (Inc., Chicago, Illinois, United States), and P-values ≤ 0.05 were considered significant.</p> <p><strong>Results</strong>: The mean age ± SD of patients was not different than that of controls (38.85 ± 5.30 Vs 40.52 ± 6.69, P=0.2). The gender was also not different (P=0.8). The mean ± SD PI of CPD patients was significantly higher (1.27± 0.34 Vs 0.60 ± 0.26, P=0.01). Similarly, the mean ± SD of GI was also significantly higher in CPD patients (1.49 ± 0.42 Vs 0.24 ± 0.14, P= 0.01). The rate of BOP sites in CPD patients was significantly higher than that of controls (P=0.001). Also, the mean ± SD of PPD in CPD patients was 4.66 ± 0.50 mm, while the mean ± SD of CAL was 4.90 ± 1.30. Additionally, the mean No.± SD of teeth in CPD patients was significantly lower (24.72± 3.77 Vs 27.62± 0.80, P=0.01). Furthermore, the mean No. ± SD of sites in CPD patients was also significantly lower (98.95± 15.08 Vs 110.50 ± 3.22, P 0.01). The mean concentration ± SD of salivary IL-6 was not higher in CPD patients (8.17 ± 3.79 Vs 7.45± 1.51, P= 0.4). qPCR results found that there was significantly higher expression of miRNA-146a in CPD patients versus that of the controls (1.79 ± 0.10 Vs 1.08± 0.10, P=0.001).</p> <p><strong>Conclusion:</strong> The current results concluded that both IL-6 and miRNA 146a were elevated in CPD patients and closely correlated with periodontal parameters suggesting that these biomarkers can be used as a surrogate diagnostic and prognostic indicator of chronic periodontitis.</p> Suha Khaleel Ibrahim, Ghada Bouslama, Lamia Oualha Copyright (c) 2025 Suha Khaleel Ibrahim, Ghada Bouslama, Lamia Oualha https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/843 Tue, 01 Apr 2025 00:00:00 -0400 Molecular Detection of Tetracycline-Resistant Streptococcus viridans Bacteria Using the rpoA Gene https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/835 <div> <div> <p class="AbstractText"><strong>Objective: </strong>The aim of this study was to established diagnostic approaches to dental caries using polymerase chain reaction technology.</p> <p class="AbstractText"><strong>Methods: </strong>PCR series. 60 samples of oral bacteria were collected between 8/6/2023 and 12/1/2023, where 27 teeth showed a growth rate for bacterial culture (45%). The bacterial isolates under study were characterized. The sensitivity of the bacterial isolates of <em>Streptococcus viridans</em> under study to eight antibiotics was tested. The results of the current study showed that the resistance rates were as follows: 71.4% for tetracycline, 57% for augmentin, 57% for ciprofloxacin, 28% for clindamycin, 57% for erythromycin, 57% for doxycycline, and 28% for doxycycline for clarithromycin.</p> <p class="AbstractText"><strong>Results</strong>: The minimum inhibitory concentration (MIC) for the bacterial isolates under study was determined for the tetracycline antibiotic. <em>S. viridans</em>isolates showed resistance to this antibiotic through a sensitivity test using the disk method, as the MIC value. for the isolates ranged from (1024-8) micrograms/m l.</p> <p class="AbstractText">The percentage of S<em>. viridans</em> isolates producing virulence factors was as follows: protease enzyme 42%, and membrane protease. Bioactive 71%, bacteriocin 14%, hemolycin 57%, DNAase 71%, capsule 28%, and lipase 28%.</p> <p class="AbstractText"><strong>Conclusion:</strong> The total DNA of all bacterial isolates under study was extracted, after which a polymerase chain reaction (PCR) was performed for the S. viridans resistant to tetracycline and with an MIC value higher than 64 µg/ml using specialized primers targeting the specific sequence of the tetM gene with a size of 1,862bp. When the amplified products were migrated on an agarose gel, one band appeared in all tracks in the gel at the same level for all samples. The results showed that the presence of the tetM gene was 100%.</p> </div> </div> Aseel Jalil Ibrahim Al-Karawi, Abdulhameed Salim Hameed, Sally Talib Da'aj, Hadeel J. Ibrahim Copyright (c) 2025 Aseel Jalil Ibrahim Al-Karawi, Abdulhameed Salim Hameed, Sally Talib Da'aj, Hadeel J. Ibrahim https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/835 Tue, 01 Apr 2025 00:00:00 -0400 Phytochemical Analysis and Antibacterial Activity of Stevia Rebaudiana Bertoni Leaves Extract against Streptococcus sanguis (A Primary Inhabitant of Dental Plaque): In Vitro Study https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/784 <div> <div> <p class="AbstractText"><strong>Objective:</strong> Dental plaque is considered the primary causative agent in developing periodontal diseases. Early colonizers of dental plaque, such as Streptococcus sanguis (S.sanguis), are crucial in the succession steps of biofilm formation. As an alternative to the commonly used chlorohexidine (CHX), it is of interest to find naturally occurring antimicrobial substances from plants.</p> <p class="AbstractText"><strong>Materials and Methods:</strong> Volunteers were asked to provide plaque samples. Microscopic examination, gram stain, optochin test, catalase test and polymerase chain reaction were used to ensure the identification of S. sanguis. Stevia Rebaudiana Bertoni leaves extracted by 70% ethanol alcohol. Four experiments have been done in this study: the susceptibility of S. sanguis to stevia extract, the minimum inhibitory (MIC) and the minimum bactericidal (MBC) concentrations, and exploration of the extract effective constituents by using HPLC.</p> <p class="AbstractText"><strong>Results:</strong> Stevia extract had good antibacterial activity with varying inhibition zone diameters that were concentration dependent, but 0.2% CHX showed better activity with a statistically significant difference (p &lt; 0.05). Both MIC and MBC were at 16 mg/ml. HPLC analysis confirmed the presence of antibacterial constituents: narigenin 25.76 ppm, catechin 30.25 ppm, coumarin 25.47 ppm, and kaempferol 4.59 ppm.</p> <p class="AbstractText"><strong>Conclusions:</strong> The antimicrobial activity of the alcoholic Stevia Rebaudiana Bertoni leaf extract was satisfactory. The study extract exhibited lower antibacterial activity at 512 mg/ml of stevia extract, while 0.2% CHX had superior activity overall. HPLC showed that the alcoholic leaves extract of Stevia Rebaudiana Bertoni contains several active antibacterial components: narigenin, catechin, coumarin and kaempferol.</p> </div> </div> Manar Ibrahim Ahmed, Safa Ali Hamad, Maha Abdulsalam Copyright (c) 2025 Manar Ibrahim Ahmed, Safa Ali Hamad, Maha Abdulsalam https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/784 Thu, 06 Feb 2025 00:00:00 -0500 The Rehabilitation of Severely Damaged Tooth Through Using a Customized Cast Post https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/828 <div> <div> <p class="AbstractText">Posts have been proposed to reinforce weak teeth that have undergone endodontic treatment, by transferring twisting pressures through the dentin of the tooth roots to the surrounding supporting tissue. The subsequent case studies applied an interdisciplinary approach in utilizing custom cast posts to restore severely damaged treated teeth, resulting in the complete restoration of their appearance and functionality. Ensuring a satisfactory outcome and satisfying the patient required the careful coordination of prosthetic and endodontic treatments, considering the patient's expectations and needs. Traditionally, a durable metal post and core were commonly employed over an extended duration to serve as the foundational restoration for a prosthetic crown. The cast post and core system offer the advantage of having the core seamlessly integrated with the post. The purpose of the post is to maintain the core restoration, which involves rebuilding the damaged coronal structure. This article discusses the use of cast post and core and zirconia restorations for a maxillary left premolar that has been damaged.</p> </div> </div> Maher M. Jwaid, Qabas Alanni, Zainab Tariq Al-Atya, Rand Mohammed Falah, Ali Radhi Ibrahim Copyright (c) 2025 Maher M. Jwaid, Qabas Alanni, Zainab Tariq Al-Atya, Rand Mohammed Falah, Ali Radhi Ibrahim https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/828 Fri, 11 Apr 2025 00:00:00 -0400 Evaluation of Postoperative Pain Intensity Following the Use of Three Different Canal Preparation Systems: A Randomized Clinical Trial https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/777 <div> <div> <p class="AbstractText"><strong>Objective:</strong> The objective of this study was to compare the intensity of postoperative pain after Hand Protaper preparation, Wave One Gold and a HyFlex EDM instrument.</p> <p class="AbstractText"><strong>Materials and Methods:</strong> Thirty patient were randomly divided into three groups. Endodontic treatment was performed for premolar and molar root canals, and they were filled with gutta-percha and an epoxy resin-based root canal sealer using a lateral condensation technique. Teeth were restored using a resin composite material. A single operator performed the treatments in a single visit for asymptomatic molars, and the patients were asked to record their pain severity during a 12, 24, 36, and 72 h follow-up period using a visual analog scale (VAS). One-way ANOVA and Tukey’s multiple comparison test were used for statistical comparisons.</p> <p class="AbstractText"><strong>Results</strong>: Up to 12 hours, postoperative pain was significantly higher in the group treated by HyFlex EDM (p &lt; 0.01). No differences were seen from 36 hours to 7 days.</p> <p class="AbstractText"><strong>Conclusion:</strong> For all three techniques, higher pain intensity was seen at 12 hours and then the pain steadily decreased. No significant difference was noted in quality of life, suggesting the filling systems or techniques has a similar effect.</p> </div> </div> Salma Issa Daheem Copyright (c) 2025 Salma Issa Daheem https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/777 Wed, 12 Feb 2025 00:00:00 -0500 Some Properties of 3D Printed Acrylic Resin Modified by Antifungal Vitis Vinifera Oil https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/873 <div> <div> <p class="AbstractText"><strong>Objective:</strong> The aim of the study was to study the consequences of different ratio of Vitis vinifera oil on the surface hardness, surface roughness and color change of 3D printed resin material<strong>.</strong></p> <p class="AbstractText"><strong> Material and Methods</strong>: 80 samples have been intended according to ADA specification 40 samples for hardness and roughness test and 40 samples for color change test, these two groups subdivided into four groups based on Vitis vinifera oil concentration 1%, 2%, 3% and control group without adding oil (0%). For each group, 10 specimens were prepared from 3D printer acrylic resin denture base, hardness was tested by shore D tester, Roughness test was done by TR 200 portable tester and color change test by spectrophotometer.</p> <p class="AbstractText"><strong>Results:</strong> The control group recorded the maximum mean value for hardness and 3% Vitis vinifera recorded the lowest value, while for roughness and color test the minimum value recorded by control and 3% Vitis vinifera oil showed the maximum value for them, for all test the differences between control and 1% were not significant, but there were significant differences with 2% and 3%.</p> <p class="AbstractText"> <strong>Conclusion:</strong> The addition of 1% Vitis vinifera oil (antifungal oil) showed no adverse effect in hardness, roughness and color change value of 3D printer acrylic resin in comparison to control group.</p> </div> </div> Amal Abdul Latif Rashid Copyright (c) 2025 Amal Abdul Latif Rashid https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/873 Fri, 18 Apr 2025 00:00:00 -0400 Efficacy of Intraoral and Extraoral Low-Level Laser Therapy (LLLT) in Managing Pain and Swelling After Surgical Removal of Impacted Mandibular Third Molar https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/814 <div> <div> <p class="AbstractText"><strong>Objective</strong>: Postoperative edema and pain are common third molar surgery side effects, that normally happens with local anesthesia. The low-level laser (LLLT) method has been universally recognized as a cell bio-modulator that is employed to accomplish optimum beneficial effects. It reduces the pain reaction, stimulates local microcirculation and wound healing, and promotes a fast healing, thus enhancing the patient's quality of life. We intended to ascertain the LLLT efficacy in improving mouth opening following the impacted third molar extraction in this research.</p> <p class="AbstractText"><strong>Methods:</strong> This randomized clinical trial was carried out on ninety cases who had impacted mandibular third molars in similar positions. Group A was allocated to intra-oral LLLT, Group B to extra-oral LLLT, and Group C to the control group. All participants were evenly divided into these three categories. The outcome that was assessed was the pain degree, alongside the postoperative edema and recovery at the operation site.</p> <p class="AbstractText"><strong>Results</strong>: Postoperative pain and edema were calculated on 1st and 7th day. All these parameters were lower in LLLT patients (p&gt;0.05).</p> <p class="AbstractText"><strong>Conclusions</strong>: After the impacted mandibular lower wisdom tooth surgical extraction, the LLLT application was effective in mitigating postoperative complications, for instance facial edema and pain.</p> </div> </div> Abdulhameed Salim Hameed, Hajer Ali Ahmed Hentati Copyright (c) 2025 Abdulhameed Salim Hameed, Hajer Ali Ahmed Hentati https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/814 Wed, 12 Feb 2025 00:00:00 -0500 Impact of Rubbing and Waiting Technique of a Universal Adhesive System on Shear Bond Strength of Orthodontic Brackets: A Comparative Analysis https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/812 <div> <div> <p class="AbstractText"><strong>Background: </strong>One important factor in orthodontics to consider is the bracket's bonding strength to the teeth substrate. This study's objective is to see how the rubbing and waiting approach of a universal adhesive system affects the shear bond strength and adhesive remnant index (ARI) of orthodontic brackets.</p> <p class="AbstractText"><strong>Materials and Methods: </strong>This was an experimental study (in vitro). Fifty newly extracted human maxillary first premolars were divided into five groups of 10 teeth each. The universal adhesive was applied on the buccal surface with 20 seconds of rubbing followed by 0, 5, 10, 15, or 20 seconds of waiting protocol according to their group. The teeth were set into blocks of acrylic resin, and the shear bond strength was tested. Afterwards, ARI was assessed. Statistical analyses were performed using the one-way analysis of variance and Tukey HSD post hoc. In addition to the Kruskal-Wallis test.<strong> </strong></p> <p class="AbstractText"><strong>Results: </strong>One-way ANOVA showed significant differences (p=0.000) between the groups. The maximum shear bond strength was for group 5 (18.93 ± 2.82), with significant differences from group 1 (p= 0.000) and group 2 (p= 0.006). The minimum bond strength was for group 1 (11.09 ± 1.50), with significant differences from all the other groups. ARI scores revealed no significant differences between the groups (p= 0.406).</p> <p class="AbstractText"><strong>Conclusion: </strong>By allowing for adequate solvent evaporation and monomer infiltration—both of which are essential for the clinical outcome of orthodontic treatment—extending the waiting period improves the shear bond strength. The ARI was not significantly impacted by waiting time.</p> </div> </div> Zahraa Mohammed Al-Fadhily, Selma Merza Hasan, Rhaf Essam Naji, Hayder Sahib Kandwh Copyright (c) 2025 Zahraa Mohammed Al-Fadhily, Selma Merza Hasan, Rhaf Essam Naji, Hayder Sahib Kandwh https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/812 Thu, 13 Feb 2025 00:00:00 -0500 Retrospective Evaluation of Management Strategies for Zygomatic Complex Fractures: Surgical vs. Nonsurgical Interventions https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/845 <p><strong>Objectives: </strong>The goal of the study was to evaluate the effectiveness of surgical and nonsurgical therapies in the treatment of zygomatic complex fractures after a year.</p> <p><strong>Materials and Methods: </strong>There were 100 patients with zygomatic complicated fractures in total; 50 of them underwent surgery and 50 underwent nonsurgical therapy. The one-year follow-up investigated aesthetic and practical elements, such as malar symmetry, ocular movement, occlusion, mouth opening, complications, and neurosensory impacts.</p> <p><strong>Results: </strong>The findings showed that 45 of the 46 patients who received surgical intervention had appropriate face contour and malar alignment. Each patient maintained enophthalmos-free normal eye movement and posture. There was tolerable occlusion, and a 49 mm average mouth opening was achieved without pain. One patient experienced minor ectropion, and five patients developed wound infections. Persistent infraorbital neurosensory abnormalities affected 19 subjects. After a year, radiographic analysis showed that all patients had excellent facial contour and adequate fracture alignment. However, the orbital floor placements of three individuals who underwent orbital reconstruction varied. Notably, neither orbital floor problems nor zygomatic complex problems necessitated reoperations or additional modifications in any patients.</p> <p><strong>Conclusions: </strong>The study concludes that nonsurgical treatments are preferable for nondisplaced fractures while surgical intervention is useful for depressed zygomatic complex fractures. For most of occurrences, an intraoral approach with firm obsession at the zygomaticomaxillary support is adequate. A second obsession technique including openness of the zygomaticofrontal intersection or sub-par orbital edge is expected for severely uprooted breaks. This inside and out assessment offers accommodating bits of knowledge into the results and treatment decisions for zygomatic confounded breaks.</p> Mohammed Rhael Ali, Azhar Abed Oudah, Basim Ali Qasim, Amran M. Al-Erjan, Qais R. Lahhob, Mustafa Mudhafar Copyright (c) 2025 Mohammed Rhael Ali, Azhar Abed Oudah, Basim Ali Qasim, Amran M. Al-Erjan, Qais R. Lahhob, Mustafa Mudhafar https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/845 Wed, 23 Apr 2025 00:00:00 -0400 Relation of Amelogenin Gene Polymorphisms to Dental Caries Among Iraqi Teenagers https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/842 <p><strong>Objective: </strong>To examine the relationship between dental caries and single nucleotide polymorphisms (SNPs) in AMELX gene.</p> <p><strong>Methods: </strong>This study was a comparative cross- sectional study encompassing 78 adolescents (aged 13-15 years) was conducted. All of them were examined for their oral and dental status under the WHO recommended criteria, and clinical information such as DMFT and DMFS were evaluated. Individuals whose DMFT and DMFS index lower than 2 were designated 'very low caries experience' and higher than 9 were designated 'higher caries experience'. Genomic DNA was extracted from saliva samples, and single nucleotide polymorphisms of AMELX were genotyped. Genotyping of three SNPs (rs17878486, rs5933871, rs5934997, intron) in AMELX gene was determined by direct sequencing and analyzed with SNPStats. Chi-square was used to compare allele and genotype frequencies between cases and controls.</p> <p><strong>Results</strong>: There was no significant difference between the high and low caries groups but in high caries group, it showed a deviation from Hardy-Weinberg equilibrium with a significant difference among the three genotypes (p=0.013) in rs5933871 and rs5934997, while a highly significant difference in rs17878486 for both high and low caries groups (p=0.000 and p=0.001). A highly significant and positive correlation between rs17878486 with DMFS in the control group and a positive significant correlation between rs17878486 and DMFT.</p> <p><strong>Conclusion:</strong> Although no significant difference of the investigated SNPs of AMELX between high and low caries groups, but rs17878486 had a significant and positive correlation with DMFT and DMFS which made this SNP as marker for caries susceptibility.</p> Elaf Abdulkareem Alidan, Ahlam Taha Mohammed, Batool Hassan Al-Ghurabi Copyright (c) 2025 Elaf Abdulkareem Alidan, Ahlam Taha Mohammed, Batool Hassan Al-Ghurabi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/842 Fri, 02 May 2025 00:00:00 -0400 Parents' Perspectives and Awareness Concerning Oral Health Care of Pediatric Patients Receiving Chemotherapy https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/830 <p><strong>Objective:</strong> Assess and determine the relationship between the parent’s awareness and perspectives on the oral health of their children receiving chemotherapy and demographics data.</p> <p><strong>Methods: </strong>A descriptive (cross-section) design study conducted in Holly Karbala Teaching Hospitals (Al-Turkish and AL-Wareth Oncology) from 24th of November 2023 to 30th June 2024.</p> <p><strong>Results</strong>: This study showed that 57% of participating parents had a low level of awareness regarding oral care, 49% regarding gum and tooth care, and 46% regarding nutrition. The study also found that 49% of parents had a low level of awareness overall regarding pediatric patients receiving chemotherapy need oral health care and the Correlation show that it was significant correlation among parents' awareness (r= 0.9, p = 0.000).</p> <p><strong>Conclusion:</strong> There is a low level of awareness of parents of children undergoing chemotherapy regarding their oral health.</p> Ali Najed Fandy, Kahtan H. Hussein Al Jebori Copyright (c) 2025 Ali Najed Fandy, Kahtan H. Hussein Al Jebori https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/830 Wed, 23 Apr 2025 00:00:00 -0400 The Effect of Cross-Section of Three Different Files on Apical Debris and Irrigant Solution Extrusion Using Full File Sequences: An In Vitro Study https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/791 <p style="font-weight: 400;"><strong>Objective: </strong>The aim of the study was to evaluate the cross-sectional geometry of heat-treated endodontic rotary files upon debris ejection through apex and the quantity of irrigants expelled when employing a complete sequence file system.</p> <p style="font-weight: 400;"><strong>Methods: </strong>Sixty extracted human maxillary first premolars were included and randomly classified into three categories (n = 20) according to the file employed during the preparation of canal. The groups were Group 1: EdgeFile X7, Group 2: Komet FQ, and Group 3: Fanta AF F One. The modified Myers along with Montgomery experiment protocol was employed to measure the mean weight of upward extruded irrigants and detritus in grams. The comparison of irrigants and debris extrusion was performed utilizing analyses of variances (ANOVA). Significant level has been established at 0.05.</p> <p style="font-weight: 400;"><strong>Results: </strong>Apical ejection of material was prevalent throughout every one of analyzed files. Quantity of debris extruded during canal shaping did not show any significant variation among the examined files, The Fanta AF F One group exhibited the greatest mean of extrusion irrigant.</p> <p style="font-weight: 400;"><strong>Conclusion: </strong>Each evaluated endodontic rotary devices produced comparable apical debris ejection among the groups. The Fanta AF F One group extruded the most irrigant.</p> Sohaib Fadhil Mohammed, Sura Yaseen Khudhur, Zaidoon Hasan Mohammed, Matheel AL-Rawas, Tahir Yusuf Noorani, Mohd Firdaus Yhaya Copyright (c) 2025 Sohaib Fadhil Mohammed, Sura Yaseen Khudhur, Zaidoon Hasan Mohammed, Matheel AL-Rawas, Tahir Yusuf Noorani, Mohd Firdaus Yhaya https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/791 Thu, 13 Feb 2025 00:00:00 -0500 Physiological Status of Some Selected Salivary Antioxidants in Dental Caries https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/774 <div> <div> <p class="AbstractText"><strong>Objective:</strong> Uric acid and total protein are the two main antioxidants in saliva, and dental caries is the oral disease that affects most of the total population. This study aimed to investigate the association between salivary antioxidants (uric acid and total protein) and dental caries in individuals between the ages of 20 and 30.</p> <p class="AbstractText"><strong>Materials and Methods</strong>: 50 subjects (18 males and 32 females) between the ages of 19 and 30 who attended the Efraz Health Center in Samarra city were recruited. Dental caries severity was evaluated using the DMFT and DT indices in accordance with WHO guidelines from 1997. Dental caries was divided into three categories based on scores: mild (0–10), moderate (11–20), and severe (21–and more). Salivary uric acid and salivary total protein concentration were assessed via chemical analysis of unstimulated saliva sample collection. The statistical package for social science (SPSS) version 21 was used to examine all data.</p> <p class="AbstractText"><strong>Results:</strong> Males had greater mean values for the DMFT and DT fractions than females, with the differences being statistically significant (p values of 0.05 and 0.001, respectively). Males also had higher salivary uric acid than females (P value of 0.001) and uric acid was associated with dental caries experience.</p> <p class="AbstractText"><strong>Conclusion:</strong> Salivary uric acid may act as a biomarker of dental caries. We found higher dental caries among subjects who had high levels of antioxidants.</p> </div> </div> Sulafa Khair Al-Deen Banoosh Alayoubi, Intesar Jasim Al-Kaysi, Hadeel Ayad, Esraa Azawi Copyright (c) 2025 Sulafa Khair Al-Deen Banoosh Alayoubi, Intesar Jasim Al-Kaysi, Hadeel Ayad, Esraa Azawi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/774 Fri, 11 Apr 2025 00:00:00 -0400 Assessment of Patient Satisfaction and The Reasons for Crowns and Fixed Partial Denture Failure, a Clinical Study https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/869 <div> <div> <p class="AbstractText"><strong>Aims. </strong>This study aimed to assess the satisfaction levels of patients who had fixed dental prostheses for rehabilitation and analyze the aspects, including biological, mechanical, and aesthetic, that contribute to the failure of these prostheses.<strong> Material and Methods.</strong> Cross-sectional observational research was undertaken in the Department of Prosthodontics, College of Dentistry, University of Basrah, Iraq, from September 2022 to May 2023. The study included a cohort of 130 patients, both males and females, who received treatment with fixed dental prostheses. The participants who were selected answered a series of questionnaires on their concerns about the fixed prosthesis, and a clinical examination involving radiographic assessments of the prosthesis was conducted. The reason for failure was documented, and data were organized for descriptive analysis of the components assessed through chi-square and Fisher's exact test. <strong>Results.</strong> Based on clinical and radiographic assessment, it was determined that 103 out of the total number of individuals assessed experienced some form of failure. 44.6% of the cases exhibited biological problems, 31% exhibited mechanical failures, and 24.2% exhibited aesthetic problems. Dental caries accounted for most biological failures (32.6%), whereas prosthesis loosening was the primary mechanical cause of failure (56.2%). Poor marginal fit was identified as the leading aesthetic reason for failure (48%). A strong correlation was seen between the level of satisfaction and mechanical failure (p=0.017).<strong> Conclusion.</strong> Most patients expressed satisfaction following the observation period. Dental decay was the most prevalent biological component leading to failure, whereas loss of retention was the primary mechanical cause. In comparison to other aspects affecting aesthetics, poor marginal fit had a more significant role.</p> </div> </div> <p> </p> Majed Mohamed Refaat Copyright (c) 2025 Majed Mohamed Refaat https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/869 Fri, 11 Apr 2025 00:00:00 -0400 Evaluation of Microhardness of self-adhesive Pediatric Filling https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/822 <div> <div> <p class="AbstractText"><strong>Objective</strong>: The purpose of this study was to evaluate how beverages affected the self-adhesive hybrid pediatric cement's microhardness.</p> <p class="AbstractText"><strong>Materials and Methods:</strong> Specimens measuring 5 mm in diameter and 2 mm in thickness were fabricated from Surefil one self-adhesive hybrid GIC and subjected to exposure by acidic beverages. The study involved three groups (artificial saliva as control, Cola, Sparkling water with lemon). Samples for Vickers hardness were immersed for 15 minutes daily for 28 days. Then, samples were tested using a Vickers microhardness tester. Statistical analysis involved the use of one-way Anova and post hoc Tucky test at the level of significance of 0.05.</p> <p class="AbstractText"><strong>Results</strong>: When Surefil One samples were submerged in simulated saliva, their Vickers microhardness significantly decreased. Samples submerged in Cola showed the largest decline, indicating a negative impact on material integrity.</p> <p class="AbstractText"><strong>Conclusions</strong>: Beverages affected the self-adhesive hybrid pediatric cement's microhardness.</p> </div> </div> Sohaib Qais Alwan, Rusul Saad Ahmed, Ali Saad Ahmed, Saif Saad Kamil Copyright (c) 2025 Sohaib Qais Alwan, Rusul Saad Ahmed, Ali Saad Ahmed, Saif Saad Kamil https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/822 Mon, 17 Feb 2025 00:00:00 -0500 Anatomical Changes Related to Age in Maxillary Sinus https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/772 <div> <div> <p class="AbstractText"><strong>Objective</strong>: The paranasal sinuses consist of four paired cavities: maxillary, ethmoid, frontal, and sphenoid, which are air-filled, mucosa-lined spaces within the maxillofacial region, connected to the nasal cavity. The maxillary sinus, the largest of these, occupies the body of the maxilla and has a pyramidal shape.</p> <p class="AbstractText"><strong>Methods:</strong> In this study, CBCT scans from 35 subjects (18 males and 17 females) who visited the University Dental Hospital in Tikrit and a private CBCT center in Samarra were analyzed. Subjects were categorized into four groups (A, B, C, and D). Measurements focused on determining the dimensions of the right maxillary sinus, including height (distance from the superior wall to the inferior wall in the coronal plane), width (distance from the lateral to the medial wall), and depth (antero-posterior distance in the axial plane). Mean values for each dimension were calculated.</p> <p class="AbstractText"><strong>Results:</strong> The study results revealed that the length and height of the right maxillary sinus (RMS) increased up to Group C and then began to decline in Group D. Male subjects showed larger sinus dimensions compared to females. In females, the maxillary sinus length and height were variable across Groups A and B, with an increase in Group C followed by a decrease in Group D, while width showed an initial fill in Group A and then a gradual decline through Groups B, C, and D. In males, length, width, and height of the sinus increased in Groups A and B, peaked in Group C, and declined in Group D.</p> </div> </div> Noor Ghazi Saab Copyright (c) 2025 Noor Ghazi Saab https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/772 Mon, 10 Feb 2025 00:00:00 -0500 Study the Effect of Chemical Solutions on Disinfection in Prosthodontics Clinic Using Digital Ultrasonic Cleaner https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/818 <div> <div> <p class="AbstractText"><strong>Objective</strong>: The disinfection measures used by dentists are inadequate and require the education and enhancement of dental practitioners’ skills to improve the safety of patients receiving treatment at dental offices. In a medical setting, the remaining bacteria that naturally exist on a patient’s body, known as endogenous flora, often leads to the transmission of infections. The objective of this study was to identify a simple and precise technique for disinfecting a prosthodontics clinic through the utilization of a chemical solution.</p> <p class="AbstractText"><strong>Methods</strong>: Swabs were collected from the patients who wear a complete denture who were contaminated, both before and after being exposed to a sterilization system. By measuring the turbidity and absorbance at 620nm using a UV spectrophotometer, we can determine the characteristics of the broth after it has been incubated at 36.5°C for 24 hours. Utilized a solution containing 0.5% sodium hypochlorite and 2% chlorhexidine. The bacterial growth was subjected to two rounds of exposure to two chemical disinfectants, each for durations of 90 seconds and 180 seconds respectively.</p> <p class="AbstractText"><strong>Results</strong>: The bacterial growth exhibited a decrease after being treated to a chemical disinfectant in a time-dependent way.</p> <p class="AbstractText"><strong>Conclusion</strong>: This study determined that the chemical solution effectively reduces bacterial development within a short period of time, providing the most convenient, rapid, and precise approach for disinfecting dental materials.</p> </div> </div> Hala M. Al-Kateb Copyright (c) 2025 Hala M. Al-Kateb https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/818 Fri, 14 Feb 2025 00:00:00 -0500 Studying Water Absorption, Solubility, and UV Absorption of Room Temperature Vulcanized Maxillofacial Silicone after the Addition of Strontium Titanate Nano-Powder https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/846 <p><strong>Objective: </strong>The study's primary goal is to assess how adding strontium titanate (SrTiO3) nanoparticles to VST-50 room temperature-vulcanized silicone elastomer affects water absorption, solubility and UV light absorption.</p> <p><strong>Materials and Method</strong>: Two weight percentages (1% and 1.5% SrTiO3) were chosen and incorporated into the VST-50 silicone. Sixty specimens were collected and split in half. There were 30 samples tested for water and solvent solubility and 30 samples tested for UV light penetration. Applying a one-way ANOVA and post hoc p .05. &lt; 0.05. Scanning electron microscope and Fourier transform infra-red were also used in the study.</p> <p><strong>Results:</strong> There was an increase in the solubility and amount of water absorbed by the experimental groups (1% and 1.5%). Also, the UV light absorption was significantly increased for the experimental groups.</p> <p><strong>Conclusion:</strong> SrTiO3 nanoparticle's addition into the VST-50 silicone increased water absorption, solubility, and UV light absorption.</p> Safwan Abid-Alhameed Suliman, Nusaiba Mustafa Muhammad, Rusal Saad Ahmed Copyright (c) 2025 Safwan Abid-Alhameed Suliman, Nusaiba Mustafa Muhammad, Rusal Saad Ahmed https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/846 Thu, 03 Apr 2025 00:00:00 -0400 Microbial Insights in Dentistry: Implications for Oral and Systemic Health https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/802 <p>The relationship between microbiology and dentistry is a cornerstone for understanding oral and systemic health dynamics. The oral cavity serves as a habitat for a diverse microbial ecosystem, which contributes to both health and disease. While a balanced oral microbiome is essential for homeostasis, its dysbiosis is implicated in dental diseases such as caries and periodontitis and systemic conditions including cardiovascular disease, diabetes, and adverse pregnancy outcomes. This review delves into the role of the oral microbiome in maintaining health, the challenges of antimicrobial resistance, and the advancements in microbial diagnostics and therapeutics. It also highlights the potential of emerging technologies and microbial-based therapies in advancing personalized dentistry. Emphasizing a multidisciplinary approach, this review provides a comprehensive understanding of how microbial research can revolutionize dental practices and patient outcomes.</p> Manar Ibrahim Ahmed, Marwa Siddik Abdulrazzaq, Huda Muneer Hashim Copyright (c) 2025 Manar Ibrahim Ahmed, Marwa Siddik Abdulrazzaq, Huda Muneer Hashim https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/802 Wed, 12 Feb 2025 00:00:00 -0500 Selected Salivary Antioxidant Levels in Relation with Periodontal Diseases https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/773 <div> <div> <p class="AbstractText"><strong>Objective:</strong> Gingivitis and periodontitis are the most common types of periodontal diseases, which are chronic inflammatory conditions encompassing many other conditions. These arise because of the interaction between a pathogenic bacterial biofilm and inflammatory chemicals and cells produced from the host.</p> <p class="AbstractText"><strong>Materials and Methods:</strong> A sample of 50 individuals (18 males and 32 females) aged 19-30 years old, treated at the health center of Efraz, Samarra city, was recruited. Plaque index was measured to determine dental plaque thickness. A calculus index was measured as well. Salivary uric acid and salivary total protein content were assessed chemically using unstimulated saliva. The statistical package for social science (SPSS) version 21 was used to examine all data.</p> <p class="AbstractText"><strong>Results and Conclusion:</strong> No associations were found between periodontal diseases and uric acid and salivary total protein content.</p> </div> </div> Sulafa Khair Al-Deen Banoosh Alayoubi Copyright (c) 2025 Sulafa Khair Al-Deen Banoosh Alayoubi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/773 Wed, 12 Feb 2025 00:00:00 -0500 Histological Evaluation of Cutaneous Wound Healing Treated by Local Application of Cucurbita pepo L. Seed Oil https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/821 <div> <div> <p class="AbstractText"><strong>Objective: </strong>The rising demand for natural medicines in pharmaceuticals has prompted scientists to investigate medicinal plants. Pumpkin seed (Cucurbita pepo L.) is a noteworthy candidate due to its remarkable pharmacological qualities for possible wound healing therapies.</p> <p class="AbstractText"><strong>Methods: </strong>A total of twelve mature male New Zealand rabbits weighing between 1.5 and 2 kg were subjected to uniform wounds on their dorsum. These rabbits were then split into two groups for healing durations of 3 and 7 days, with 6 rabbits in each group. The induced wound in the control group was allowed to heal naturally, whereas the wounds in the experimental group were treated with a daily local application of pumpkin oil (10µl). The animals were sacrificed after healing times of three and seven days. All examined groups underwent clinical assessment of wound contraction. Specimens were obtained for histological and histomorphometric analysis to evaluate inflammatory cell and blood vessel counts, as well as epithelial thickness.</p> <p class="AbstractText">Results: The average percentage of wound contraction escalated over time. A notable change was found and a statistically significant disparity in the mean values of inflammatory cells was recorded. The mean values for blood vessel count rose with a notable disparity was noted between the control and experimental groups. The evaluation of epithelial thickness indicated that mean values increased over time, with a significant difference in both experimental groups.</p> <p class="AbstractText">Conclusion: As a potential therapeutic agent for wound healing, pumpkin oil may be also beneficial for nutritional and therapeutic applications.</p> </div> </div> Ibrahim Fouad Mohamed, Ban A. Ghani , Nada M.H. Al-Ghaban , Abdalbseet A. Fatalla , Matheel Al-Rawas Copyright (c) 2025 Ibrahim Fouad Mohamed, Ban A. Ghani , Nada M.H. Al-Ghaban , Abdalbseet A. Fatalla , Matheel Al-Rawas https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/821 Mon, 17 Feb 2025 00:00:00 -0500 Salivary Caries-Related Microorganisms and Demographics of a Group of Children https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/764 <div> <div> <p class="AbstractText"><strong>Objective: </strong>The purpose of this study was to determine the association between caries related microorganisms in children<sup>’</sup>s saliva, such as <em>Streptococcus mutans</em> and <em>lactobacilli</em>, and their demographic factors.</p> <p class="AbstractText"><strong>Methods: </strong>This study involved a sample of 135, both sexes with an age range between 3 and 10 years. Unstimulated saliva was obtained and diluted in normal saline. Saliva was then placed in selective media. Salivaris agar was used for <em>mutans streptococci</em> while Rogosa agar for<em> lactobacilli</em>. After incubation, <em>Streptococcus mutans</em> counting of CFU (colony forming units) with morphology characterization and numbers of CFU per milliliter of saliva for <em>lactobacilli</em>. Demographic factors information was collected using a questionnaire.</p> <p class="AbstractText"><strong>Results</strong>: Both studied salivary microorganisms increased with age. <em>Streptococcus mutans</em> was higher in boys, and associated with breastfeeding, overweight, and not brushing their teeth. L<em>actobacilli</em> were higher in girls, and associated with mothers who were government employee, mother occupation, children who during sleep were not nursed, and with normal weight.</p> <div> <div> <p class="AbstractText"><strong>Conclusion:</strong> The present study found an association between salivary <em>Streptococcus mutans</em> and <em>lactobacilli</em> and various demographic variables in a group of children.</p> </div> </div> </div> </div> Alhan Ahmed Qasim, Muna Saleem Khalaf, Ghada Ibrahim Taha, Zainab Juma Jafar Copyright (c) 2025 Alhan Ahmed Qasim, Muna Saleem Khalaf, Ghada Ibrahim Taha, Zainab Juma Jafar https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/764 Wed, 15 Jan 2025 00:00:00 -0500 Effect of Menthe and Green Tea Volatile Oil Extract on Streptococcus mutans, a Bacterium Species Isolated from Dental Caries https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/860 <p><strong>Objectives: </strong>To identify phytochemical components, evaluate the yield or volatile oil extraction, and assess the impact of volatile oil extract on a strain of <em>Streptococcus mutans</em> that was isolated from a dental caries.</p> <p><strong>Materials and Methods: </strong><em>Menthe spicata</em> (menthe) and <em>Camellia sinensis</em> (green tea) were harvested fresh during the flowering period of 2022, and their volatile oils were extracted for about 24 hours using a sox let apparatus. Green tea and menthe were prepared at four different concentrations (50, 75, 100, and 150 mg/ml). <em>Streptococcus mutans</em> growth isolates were inhibited, and the results were compared with extracts from both kinds of plants.</p> <p><strong>Results: </strong>Menthe produced the most volatile oil extract (3%), while green tea produced the least (2%). Alkaloid, phenol, glycoside, and flavonoid showed positive results in the phytochemical screening of <em>M. spicata</em> volatile oil extract, but saponin showed negative results. The results also showed that the volatile oil of green tea and menthol oils (100 and 150 mm/ml) were more inhibitory than those of 50 and 75 mm/ml. Overall, given these results, volatile oil of menthe has greater effect on dental caries compared to volatile oil of green tea.</p> <p><strong>Conclusions: </strong>The volatile oil extract on menthe inhibitor is more effective on the <em>S. mutans</em> bacteria compared to volatile oil of the green tea.</p> Mohamed Abdulmunem Abdulafeet, Sura A. Jaber, Anes A. Alshamaa, Sohaib Mohameed Awad Copyright (c) 2025 Mohamed Abdulmunem Abdulafeet, Sura A. Jaber, Anes A. Alshamaa, Sohaib Mohameed Awad https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/860 Fri, 25 Apr 2025 00:00:00 -0400 Localization of E-cadherin and Vimentin in Mucosal Ulcer Healing Treated by Vitis Vinifera Seeds Oil in Rat Model https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/847 <p><strong>Objective</strong>: The anti-inflammatory components generated by Vitis vinifera (Grape) seeds oil (GSO) help reduce inflammation and accelerate the healing process when applied topically to an ulcer. The current study aimed to assess the expression of E-cadherin and Vimentin during oral ulcer healing treated by GSO.</p> <p><strong>Materials and Methods:</strong> Twenty-four albino male rats (350–450 g) were used for this study. The rats were slaughtered on the 5th and 10th days after an ulcer induction on either their left or right cheek mucosa. The rats in the control group received sterile distilled water, whereas those in the experimental group received GSO for ulcers treatment. The tissue samples were subsequently utilized for histological and immunohistochemical investigations of E-cadherin and Vimentin.</p> <p><strong>Results:</strong> Compared with the control group, the GSO-treated ulcer group presented significantly higher expression of E-cadherin and Vimentin at days 5 and 10 than the control group, with some evidence of epithelial-mesenchymal transition (EMT) at 5 days in both groups.</p> <p><strong>Conclusion:</strong> With the limitation of this study, GS may accelerate the healing of mucosal ulcers, as demonstrated by increased expression of E-cadherin and Vimentin.</p> <p><strong>Clinical relevance: </strong>GSO can be used as a topical herbal modality for mucosal ulcer healing.</p> Russol Faik, Nada M.H. Al-Ghaban Copyright (c) 2025 Russol Faik, Nada M.H. Al-Ghaban https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/847 Fri, 18 Apr 2025 00:00:00 -0400 Novel Surgical Method for Contour Restoration in Patients with Micrognathia https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/826 <div> <div> <p class="AbstractText">The bone graft procedure is the most popular approach that has been utilized to repair and shape the micrognathia. Several other treatments have also been employed. The new surgical technique is a comprehensive technique, it gives a ridge and permanent chin with a normal esthetic appearance.</p> </div> </div> Sabah Hassan Copyright (c) 2025 Sabah Hassan https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/826 Fri, 14 Feb 2025 00:00:00 -0500 Influence of Different Temperatures on the Polymerization Pre- and Post-Cured of Various Resin Materials https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/788 <div> <div> <p class="AbstractText"><strong>Objective: </strong>To evaluate and compered the effect of different temperatures (5°C, 37°C and room temp. ±23°C) pre- cured and post-cured for three universal- Chroma composite materials (Hybrid-Nano fillers, Supra-Nano, Nano filler) on the polymerization degree and micro-hardness.</p> <p class="AbstractText"><strong>Materials: </strong>A seventy-five disc-samples-shaped were fabricated from (Omnichroma, Vittra APS, DenFil N), for each test in different temperatures (5°C, 37°C and room temp. ±23°C) were light cured according to manufacture instruction. The Fourier trans-form infrared spectroscopy was used to the polymerization degree measured for each sample while the micro-hardness was measured by the using of Vickers hardness test. Data were analyzed using One-Way-Analysis of Variance at level p &lt; 0.05.</p> <p class="AbstractText"><strong>Results: </strong>The analysis showed that there was significant difference in the polymerization degree and in the micro-hardness of the samples fabricated at the different temperatures when heated pre- and post- cured of all materials increase, in the polymerization degree and the micro-hardness of the samples.</p> <p class="AbstractText"><strong>Conclusions: </strong>Increasing three universal- Chroma composite materials (Hybrid-Nano fillers, Supra-Nano, Nano filler) temperature whether pre-cured and post-cured allows for maintaining or increasing polymerization degree and hardness of three universal- Chroma composite materials especially DentfilN Nano- filler composite.</p> </div> </div> Fanar Turki Al-Jadwaa, Mhammad Munthir Abdurazzaq, Emad Farhan Alkhalidi Copyright (c) 2025 Fanar Turki Al-Jadwaa, Mhammad Munthir Abdurazzaq, Emad Farhan Alkhalidi https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/788 Wed, 12 Feb 2025 00:00:00 -0500 Effectiveness of Behavioral and Lifestyle Changes in Reducing TMD Symptoms: Evidence from a Structured Intervention https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/825 <div> <div> <p class="AbstractText"><strong>Objective:</strong> To assess the effectiveness of behavioral measures in managing TMDs with an emphasis on pain, joint mobility and psychosocial status.</p> <p class="AbstractText"><strong>Methods:</strong> Forty-eight subjects with TMDs were enrolled based on the RDC/TMD criteria. Stress management, ergonomics training, dietary changes and stretching exercises were recommended as individualized lifestyle plans. The clinical, psychosocial and functional evaluations were done before and after three months of intervention.</p> <p class="AbstractText"><strong>Results</strong>: The frequency of myofascial pain without limited mouth opening was 45. 8% which was reduced to 20. The percentage of subjects with right and left TMJ arthralgia was also seen to have reduced significantly (70. 8% to 43. 7%, p = 0. 0133; 75% to 41. 6%, p = 0. 001). Right TMJ osteoarthrosis and osteoarthritis showed a considerable improvement (p &lt; 0. 05) while the changes in the left TMJ were not significant. Disc displacement with reduction on the right and without reduction on the left side was also found to have improved markedly (p = 0. 036; p = 0. 041). Depression severity was also found to have reduced significantly with the normal levels increasing from 45. 8% to 83. 3% (p &lt; 0. 05). Pain intensity as measured by VAS also reduced from 4. 72 ± 1. 44 to 3. 43 ± 1. 38 (p = 0. 001). There was also an enhancement in the non-specific physical symptoms but the differences were not statistically significant.</p> <p class="AbstractText"><strong>Conclusions</strong>: The present study demonstrated that the implementation of lifestyle modifications helps in decreasing the symptoms of TMD, increases the joint function and has a positive effect on the psychosocial status of the patients and therefore should be considered as the first-line treatment in the management of TMD.</p> </div> </div> Dunya Malhan Hanweet, Karar Abdulzahra Mahdi, Ansam Mahdi Khalel, Salah M. Ibrahim Copyright (c) 2025 Dunya Malhan Hanweet, Karar Abdulzahra Mahdi, Ansam Mahdi Khalel, Salah M. Ibrahim https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/825 Thu, 13 Feb 2025 00:00:00 -0500 The Impact of Aloe Vera Herbal Extract on the Antifungal and Mechanical Properties of Maxillofacial Prosthetic Silicones https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/868 <p><strong>Objectives: </strong>The study aimed to evaluate the antimicrobial efficacy and some mechanical properties of maxillofacial silicone after incorporating aloe vera oil.</p> <p><strong>Materials and Methods: </strong>Two concentrations of aloe vera oil were added to the room temperature vulcanized maxillofacial silicone. Three tests were conducted (disk diffusion test, tear strength, and shore A hardness) using standardized conditions. Statistical analysis involved the use of one-way ANOVA and Tukey’s post-hoc tests.</p> <p><strong>Results: </strong>It was revealed that aloe vera oil has strong antifungal properties for 1% and 2% incorporated specimens as there was large inhibition zone (5.94 mm and 8.45 mm respectively) around the incorporated specimens compared to control specimens. Tear strength was decreased after the addition, while hardness was increased especially for 2% incorporated specimens but still within acceptable limits.</p> <p><strong>Conclusions: </strong>The study contributes valuable insights into the development of antimicrobial silicone material reinforced with Aloe vera oil, advancing maxillofacial materials towards improved patient outcomes and enhanced health.</p> Nusaiba Mustafa Muhammed, Safwan Abid-Alhameed Suliman, Luma Nasrat Arab Copyright (c) 2025 Nusaiba Mustafa Muhammed, Safwan Abid-Alhameed Suliman, Luma Nasrat Arab https://creativecommons.org/licenses/by/4.0 https://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/868 Mon, 21 Apr 2025 00:00:00 -0400