2024-03-28T14:26:57Z
http://dentistry3000.pitt.edu/ojs/dentistry3000/oai
oai:ojs.dentistry3000.pitt.edu:article/9
2017-01-06T19:37:43Z
dentistry3000:MoOD
Lack of association between IRF6 polymorphisms and nonsyndromic oral clefts in South Indian population
Gurramkonda, Venkatesh Babu
Murthy, Jyotsna
Syed, Altaf Hussain
Lakkakula, Bhaskar VKS
IRF6 gene
orofacial clefts
SNP
Objective: This present study is aimed to investigate the association between interferon regulatory factor 6 (IRF6), single nucleotide polymorphisms (SNPs), and nonsyndromic cleft lip without without cleft palate (NSCLP) in the South Indian population. Subject and Methods: For this study, 190 unrelated NSCLP patients and 189 controls without clefts were genotyped with rs2235371 (V2741) and rs642961 SNPs using PCR-RFLP. The associations between NSCLP groups and IRF6 gene polymorphisms, as well as haplotypes, were analyzed using chi-squared test and 95% confidence interval (95%CI) of the odds ratios were calculated with the control groups as reference. Results: For controls, the minor allele frequencies of both variants, V2741 and rs642961, were 7.1% and 21.1%, respectively. Genotype data for both variants in control and cleft groups follow the Hardy Weinberg Equilibrium. Between cases with NSCLP and controls, the two SNPs showed no differences in frequencies of the genotypes or alleles. The pairwise linkage disequilibrium (LD) values (D’=1 and r2=0.027) between V2741 and rs642961 revealed that these two SNPs are not in strong LD. Haplotype G-T showed a significantly reduced risk for oral clefts (p<0.001) and haplotype A-T increased the risk for oral clefts (p=0.043). Gene-gene interaction showed that the higher risk group contains more GG-CC combination of cases that the controls, but this model was not significantly associated with cleft status (p=0.136) Conclusion: In conclusion, while IRF6 is strongly associated in other populations, this study demonstrated that variants in IRF6 may play a role in NSCLP in a South Indian population, but other genes are expected to play a role in this population as well.
University Library System, University of Pittsburgh
2013-08-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
case control
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/9
10.5195/d3000.2013.9
Dentistry 3000; Vol. 1 No. 1 (2013); 25-29
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/9/6
oai:ojs.dentistry3000.pitt.edu:article/10
2017-01-06T19:37:45Z
dentistry3000:DoCS
Sexual dimorphism involved in the mesiodistal and buccolingual dimensions of permanent teeth
Sabóia, Ticiana Medeiros
Tannure, Patricia Nivoloni
Luiz, Ronir Raggio
Costa, Marcelo de Castro
Granjeiro, Jose Mauro
Küchler, Erika Calvano
Antunes, Leonardo Santos
Sex Determination
tooth crown
tooth class
Studies indicate that tooth crown diameters are clinical markers for sex differentiation. Therefore, the aim of this study was to assess the degree of sexual dimorphism in different teeth. Maximum mesiodistal (MD) and buccolingual (BL) dimensions of 2400 permanent teeth from 100 pretreatment orthodontic dental study casts and clinical records (50 males and 50 females) from the Department of Pediatric Dentistry and Orthodontics, Federal University of Rio de Janeiro, Brazil, were examined. Comparison of the MD and BL dimensions between males and females was performed using the Student’s t test with alpha 0.05, effect size, and discriminant function analysis. Comparisons in MD and BL widths between sexes demonstrated that the combined mean in the female group presented reduction when compared with the male group, except for the BL dimension of tooth 26. In regard to the MD dimensions, statistically significant differences were observed in various dental groups. The greatest sexual dimorphism was observed in the left mandibular canine (p<0.001) with effect size over 0.8 (0.94), which characterizes large effect. In BL dimension, numerous teeth demonstrated statistical differences between the sexes. Our findings reinforced the magnitude of sexual dimorphism in tooth size, and, in addition, highlighted the differences in specific dental groups.
University Library System, University of Pittsburgh
2013-11-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/10
10.5195/d3000.2013.10
Dentistry 3000; Vol. 1 No. 1 (2013); 2-6
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/10/14
oai:ojs.dentistry3000.pitt.edu:article/11
2017-01-06T19:37:46Z
dentistry3000:MoOD
Non-syndromic oral clefts and risk of cancer: a systematic review
Popoff, Daniela Araújo Veloso
Coelho, Mateus Prates
Martelli, Daniella Reis Barbosa
Saini, Rajiv
Coletta, Ricardo Della
Martelli-Júnior, Hercílio
Cleft lip and palate
cancer
family studies.
Objective: To discuss the risk of cancer among relatives of individuals with cleft lip and palate (CL/P), as well as the risk of CL/P among relatives of individuals with cancer, since studies published currently have suggested an increased risk of cancer among relatives of cleft individuals. Design: A systematic literature review was carried out in accordance with the Cochrane Collaboration Group protocol, including literature search strategy, selection of papers through the inclusion and exclusion criteria, data extraction and quality assessment. PubMed, Scopus and ISI - Web of Science databases were systematically searched using the fallowing search strings: “cleft lip and palate” AND “cancer”, “oral clefts” AND “cancer” and “orofacial clefts” AND “cancer”. Results: From 653 studies accessed, 8 comprised the final sample: 6 investigating CL/P index cases and their family history of cancer and 2 investigating individuals with cancer and their family history for CL/P. The sample sizes were not homogeneous. Oral clefts, the type of cancer and the degree of kinship family were not categorized in all studies. Leukemia, breast cancer and colon cancer were the most cited types, even as first-and-second degree relatives. Conclusions: An increased risk of cancer among relatives of cleft individuals could not be entirely confirmed. However, studies with this specific purpose suggest that first-and-second degrees relatives of cleft individuals have some types of cancer more often than unexposed families, highlighting that future studies should expand their samples to investigate possible common molecular mechanisms that allow relating oral clefts and cancer.
University Library System, University of Pittsburgh
2013-08-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Systematic review
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/11
10.5195/d3000.2013.11
Dentistry 3000; Vol. 1 No. 1 (2013); 12-18
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/11/7
oai:ojs.dentistry3000.pitt.edu:article/14
2017-01-06T19:37:47Z
dentistry3000:DoCS
Heritability of face shape in twins: a preliminary study using 3D stereophotogrammetry and geometric morphometrics
Weinberg, Seth M.
Parsons, Trish E.
Marazita, Mary L.
Maher, Brion S.
Introduction: Previous research suggests that aspects of facial surface morphology are heritable. Traditionally, heritability studies have used a limited set of linear distances to quantify facial morphology and often employ statistical methods poorly designed to deal with biological shape. In this preliminary report, we use a combination of 3D photogrammetry and landmark-based morphometrics to explore which aspects of face shape show the strongest evidence of heritability in a sample of twins. Methods: 3D surface images were obtained from 21 twin pairs (10 monozygotic, 11 same-sex dizygotic). Thirteen 3D landmarks were collected from each facial surface and their coordinates subjected to geometric morphometric analysis. This involved superimposing the individual landmark configurations and then subjecting the resulting shape coordinates to a principal components analysis. The resulting PC scores were then used to calculate rough narrow-sense heritability estimates. Results: Three principal components displayed evidence of moderate to high heritability and were associated with variation in the breadth of orbital and nasal structures, upper lip height and projection, and the vertical and forward projection of the root of the nose due to variation in the position of nasion. Conclusions: Aspects of facial shape, primarily related to variation in length and breadth of central midfacial structures, were shown to demonstrate evidence of strong heritability. An improved understanding of which facial features are under strong genetic control is an important step in the identification of specific genes that underlie normal facial variation.
University Library System, University of Pittsburgh
2013-11-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/14
10.5195/d3000.2013.14
Dentistry 3000; Vol. 1 No. 1 (2013); 7-11
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/14/15
oai:ojs.dentistry3000.pitt.edu:article/15
2017-01-06T19:37:49Z
dentistry3000:ER
Errata for “Lack of association between IRF6 polymorphisms and nonsyndromic oral clefts in South Indian population,” Volume 1, No 1
Vinski, Sarah
This page reflects errors that were found in the article “Lack of association between IRF6 polymorphisms and nonsyndromic oral clefts in South Indian population," Volume 1, No 1 of Dentistry 3000. 1. Gurramkonda, Venkatesh, Jyotsna Murthy, Altaf Hussain Syed, & Bhaskar VKS Lakkakula. "Lack of association between IRF6 polymorphisms and nonsyndromic oral clefts in South Indian population." Dentistry 3000 [Online], 1.1 (2013): n. pag. Web. 3 Sep. 2013 The article title should be “Evidence of association between IRF6 polymorphisms and nonsyndromic oral clefts in South Indian population.” Last updated Wednesday, September 4, 2013
University Library System, University of Pittsburgh
2013-09-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/15
10.5195/d3000.2013.15
Dentistry 3000; Vol. 1 No. 1 (2013); 30
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/15/9
oai:ojs.dentistry3000.pitt.edu:article/16
2017-01-06T19:37:50Z
dentistry3000:MoOD
Summary of the IADR Cariology Research, Craniofacial Biology, and Mineralized Tissue Groups Symposium, Iguaçu Falls, Brazil, June 2012: Gene-environment Interactions and Epigenetics in Oral Diseases: Enamel Formation and its Clinical Impact on Tooth Defects, Caries, and Erosion
Modesto, Adriana
Klein, Ophir
Tenuta, Livia M.A.
Gerlach, Raquel F.
Vieira, Alexandre R.
Characteristics of enamel may influence or modulate individual susceptibility to caries and erosion. These characteristics are defined during development, which is under strict genetic control, but can easily be modified in many ways by environmental factors. In the symposium, translational aspects of embryology, biochemistry, and genetics of amelogenesis were presented. The symposium provided unique insight into how basic sciences integrate with clinically relevant problems. The need for improved understanding of risks at the individual level, taking into consideration both environmental exposures and genetic background, was presented. The symposium was divided into four stepwise and interconnected topics as follows: 1) The Many Faces of Enamel Development; 2) Enamel Pathogenesis: Biochemistry Lessons; 3) Environmental Factors on Enamel Formation; and, 4) Genetic Variation in Enamel Formation Genes.
University Library System, University of Pittsburgh
2013-12-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/16
10.5195/d3000.2013.16
Dentistry 3000; Vol. 1 No. 1 (2013); 19-24
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/16/17
oai:ojs.dentistry3000.pitt.edu:article/17
2017-01-06T19:37:52Z
dentistry3000:EDT
Introducing Dentistry 3000
Vieira, Alexandre R.
University Library System, University of Pittsburgh
2013-12-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/17
10.5195/d3000.2013.17
Dentistry 3000; Vol. 1 No. 1 (2013); 1
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/17/16
oai:ojs.dentistry3000.pitt.edu:article/19
2017-01-06T19:37:53Z
dentistry3000:I%26A
Manual and rotary instrumentation techniques for root canal preparation in primary molars
Rosa, Francinne M.
Modesto, Adriana
Faraco-Junior, Italo M.
instrumentation
primary tooth
root canal therapy
Introduction: Although rotary instrumentation has been widely studied in permanent dentition, it is a rather new field of study concerning primary teeth. Purpose: We aimed to evaluate apical displacement and time needed for instrumentation of root canals of primary molars by manual and rotary techniques. Materials and Methods: Root canals of 144 extracted first and second primary maxillary molars were randomly divided into 2 groups: I- manual instrumentation (K-files); II- rotary instrumentation (K3 Rotary System®). The canals were radiographed with pathfinding files in place, prepared by both techniques, and instrumentation time was recorded. After preparation, root canals were radiographed again with pathfinding files in place. To analyze the degree of apical displacement, digital images were superimposed using the Adobe Photoshop® software. Results: Mean apical displacement (0.70 mm) in the manual instrumentation group was not statistically different from that in the rotary instrumentation group (0.79 mm). However, mean time for root canal preparation was significantly shorter using the rotary system (128.0 s) than using the manual system (174.0 s) (p<0.05). Conclusions: The use of rotary instrumentation in pediatric dentistry is feasible, offering time-saving advantages in root canal preparation.
University Library System, University of Pittsburgh
2014-05-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/19
10.5195/d3000.2014.19
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/19/19
oai:ojs.dentistry3000.pitt.edu:article/21
2017-01-06T19:37:55Z
dentistry3000:DoCS
Hypoxic conditions alter developing branchial arch-derived structures in zebrafish
Parsons, Trish E
Weinberg, Seth M
Tsang, Michael
Vieira, Alexandre R
Background: Previous epidemiological findings have implicated hypoxia as a risk factor for craniofacial defects including cleft lip, microtia and branchial arch anomalies. This study tests the hypothesis that hypoxic exposure results in craniofacial shape variation in a zebrafish model. Methods: Three sets of zebrafish embryos were raised in uniform conditions with the exception of dissolved oxygen level. At 24 hours past fertilization (hpf) embryos were placed in hypoxic conditions (70% or 50% dissolved oxygen tank water) and compared to unexposed control embryos. After 24 hours of exposure to hypoxia, the embryos were incubated under normoxia. Larvae were collected at 5 days post fertilization (dpf) and stained for cartilage. Images were taken of each specimen and subsequently landmarked to capture viscerocranial morphology. A geometric morphometric analysis was performed to compare shape variation across groups. Results: The mean branchial arch shape of each exposure group was significantly different from controls (p<0.001). Principal components analysis revealed a clear separation of the three groups, with controls at one end of the shape spectrum, the 50% hypoxia group at the other end, and the 70% hypoxia group spanning the variation in between. Conclusions: This experiment shows that hypoxia exposure at 24hpf is capable of affecting craniofacial shape in a dose-dependent manner. These results may have implications not only for high altitude fetal health, but other environments, behaviors and genes that affect fetal oxygen delivery.
University Library System, University of Pittsburgh
2014-08-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/21
10.5195/d3000.2014.21
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/21/20
oai:ojs.dentistry3000.pitt.edu:article/24
2017-01-06T19:37:56Z
dentistry3000:A%26tE
A gas adsorption porosimetry analysis of Portland cement prepared by compaction vs. compaction with indirect ultrasonic agitation
Azizi, Nader
Cubas, Yltze P
Orellana, Maria F
Gas adsorption
porosimetry
Portland cement
compaction with ultrasonic agitation and indirect ultrasonic agitation
Background: Mineral trioxide aggregate (MTA), is commonly used in endodontic and restorative procedures. Objective: Our objective was to introduce gas adsorption porosimetry as a viable method for evaluation of general porosity and specific pore characteristics of set Portland cement used in substitute for MTA, to investigate the effect of two different obturation methods (compaction and compaction in conjunction with indirect ultrasonic agitation of the cement paste), and to evaluate the correlation between the specific pore characteristics to compressive strength in general. Material and Methods: Portland cement samples were prepared and divided into two groups based on compaction techniques. An initial stereomicroscopic evaluation was done to assess any differences in appearance of pores randomly selected from either of the two experimental groups. Specific pore characteristics and compressive strength were quantified by a gas adsorption porosimeter and an Instron universal testing machine. A two tailed student t-test was used for statistical comparison of data, and a regression analysis was done to evaluate the correlation between each specific pore characteristic and compressive strength in general. Results: The gas porosimetry method provided measurable values relating to specific pore characteristics of Portland cement. The stereomicroscopy evaluation revealed marked differences between samples from the two groups, namely visibly larger pores both on the outside surface and in cross-sections of specimens prepared by the indirect ultrasonic activation method. Conclusions: Gas adsorption porosimetry is a feasible method for evaluation of specific pore characteristics of Portland cement and potentially other dental materials as well.
University Library System, University of Pittsburgh
2014-11-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/24
10.5195/d3000.2014.24
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/24/24
oai:ojs.dentistry3000.pitt.edu:article/25
2017-01-06T19:37:58Z
dentistry3000:DoCS
Prevalence of dental anomalies in a Mexican population
Herrera-Atoche, Jose Ruben
Diaz-Morales, Silvia
Colome-Ruiz, Gabriel
Escoffie-Ramirez, Mauricio
Orellana, Maria Fernanda
prevalence
dental anomalies
mexican
Background: Data on dental anomaly prevalence is instrumental to diagnosis and treatment in different populations. A retrospective study was done to determine dental anomaly prevalence and associations in a group of orthodontic patients in a Mexican population. Methods: Number, shape, eruption and structural dental anomalies were assessed from the records of 670 subjects. Prevalence, distribution, and associations between the different anomalies were calculated. Chi-squared and Fisher’s exact tests (p<0.05) were used to identify significant differences by sex, and to establish associations among the studied anomalies. Results: Twenty-eight percent of the sample exhibited at least one dental anomaly. Statistical analysis identified no differences by gender. The most common anomaly was impacted teeth (13.58%), followed by microdontic upper lateral incisors (6.26%). These two anomalies also had the highest number of significant associations with other anomalies. Conclusions: The dental anomalies prevalence documented here differ from those reported in the literature for other populations in the world. Dental anomalies are normally associated with each other and occur in groups linked to ethnic origin. The present results indicate the presence of differing suites of anomalies between the studied Mexican population and other populations in the world. This variation highlights the need for further research on dental anomalies in Latin America to aid in their diagnosis and treatment.
University Library System, University of Pittsburgh
2014-12-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/25
10.5195/d3000.2014.25
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/25/25
oai:ojs.dentistry3000.pitt.edu:article/26
2017-01-06T19:37:59Z
dentistry3000:I%26A
New electronic apex locator Romiapex A-15 presented accuracy for working length determination in permanent teeth
Maia Filho, Etevaldo Matos
Rizzi, Cláudia de Castro
Oliveira, Daniela Silva Barroso de
Filho, Paulo Nelson
Silva, Raquel Assed Bezerra da
Silva, Léa Assed Bezerra da
electronic apex foraminal
working length determination
endodontics
Purpose: The present study aims to evaluate, ex vivo, the accuracy of electronic apex locators Root ZX II and Romiapex-15, for working length (WL) determination in permanent teeth. Materials and Methods: Fourteen single-rooted teeth (incisors and canines), with their apices fully formed were used. The dental crowns were removed. The anatomic length of the tooth (real measurement) was visually determined through the insertion of a size 10 K-file until the tip of the instrument could be observed in the apical foramen under a microscope (20X). Teeth were fixed in a model of resin and adapted into alginate soaked with saline solution, which was used as an electrical conductor. Using a K-file, root canals were measured electronically using both devices. The results obtained for each apex locator were compared to the real measurements. The accuracy between the devices was statistically analyzed using the Bland-Altman graph, Intraclass Correlation Coefficient (ICC), and Student’s t-test. Results: The mean difference between the measurements using the Root ZX II was 0.277mm greater than the real measurement, while the measurements from the Romiapex-15 were 0.308mm higher on average. The comparison between Root ZX II and Romiapex-15 had no significant difference (p= 0.868). Conclusion: It was concluded that Root ZX II and Romiapex-15 had similar accuracy. Romiapex-15 could be an option for WL determination in permanent teeth.
University Library System, University of Pittsburgh
2014-12-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/26
10.5195/d3000.2014.26
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/26/26
oai:ojs.dentistry3000.pitt.edu:article/27
2017-01-06T19:38:02Z
dentistry3000:MoOD
Polymorphism in the tumor necrosis factor-alpha gene (TNFA -308 G/A) is not associated with susceptibility to chronic periodontitis in a Brazilian population
Trevilatto, Paula Cristina
de Brito Jr, Rui Barbosa
Scarel-Caminaga, Raquel Mantuaneli
Pardo, Ana Paula de Souza
de Souza, Cleber Machado
Sallum, Antônio Wilson
Line, Sérgio Roberto Peres
chronic periodontitis
polymorphisms
TNFA gene
Objective: Tumor necrosis factor-alpha (TNF-α) is a major mediator of the immune-inflammatory response and may play an important role in the pathogenesis and progression of chronic periodontitis. Polymorphisms in the promoter of the TNFA gene have been associated with some types of inflammatory diseases. The present study investigated the association between a single-nucleotide polymorphism (SNP) of the TNFA (G-308A) gene and chronic periodontitis in Brazilians. Methods: One hundred and thirteen (113) over 25 years were divided according to the severity level of periodontal disease: 44 healthy individuals (control group), 31 subjects with moderate and 38 patients with severe periodontitis. Genomic DNA was obtained from epithelial cells. The samples were analyzed for TNFA (G-308A) polymorphism using polymerase chain reaction-restriction fragment length polymorphism techniques. The significance of the differences in the genotype frequencies of the polymorphism was assessed by Chi-square test (p<0.05). Results: No significant differences in the genotype distribution and allele frequency were found between control and groups with periodontitis. Conclusion: It was concluded that TNFA (-308) polymorphism was not associated with chronic periodontitis. Other polymorphisms in this or/and other genes of the host inflammatory response might be involved in determining susceptibility to periodontitis in the study population.
University Library System, University of Pittsburgh
2016-02-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Prospective study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/27
10.5195/d3000.2015.27
Dentistry 3000; Vol. 3 No. 1 (2015); 47-52
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/27/38
oai:ojs.dentistry3000.pitt.edu:article/28
2017-01-06T19:38:00Z
dentistry3000:EDT
Sustainability is success
Vieira, Alexandre R.
None
University Library System, University of Pittsburgh
2014-12-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/28
10.5195/d3000.2014.28
Dentistry 3000; Vol. 2 No. 1 (2014)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/28/27
Copyright (c) 2014 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/29
2017-01-06T19:38:04Z
dentistry3000:I%26A
Profile of Fluoride Release from a Nanohybrid Composite Resin
Assed Bezerra Silva, Raquel
Ribeiro Santos, Fernanda Regina
Cesar Cropanese Spadaro, Augusto
Morseli Polizello, Ana Cristina
De Rossi, Andiara
Moreira, Marilia Rodrigues
Nelson-Filho, Paulo
composite resin
sodium fluoride
glass ionomer cement
The aim of this study was to evaluate in vitro the amount and profile of fluoride release from a fluoride-containing nanohybrid composite resin (Tetric® N-Ceram) by direct potentiometry. Thirty specimens (5 mm diameter x 3 mm high; n=10/material) were made of Tetric® N-Ceram, Vitremer® resin-modified glass ionomer cement (RMGIC) (positive control) or Filtek® Z350 nanofill composite resin (negative control). The specimens were stored individually in plastic tubes containing 1 mL of artificial saliva at 37°C, which was daily renewed during 15 days. At each renewal of saliva, the amount of fluoride ions released in the solution was measured using a fluoride ion-selective electrode with ion analyzer, and the values obtained in mV were converted to ppm (µg/mL). Data were analyzed statistically by ANOVA and Tukey’s post-hoc test at a significance level of 5%. The results showed that the resins Tetric® N-Ceram and Filtek® Z350 did not release significant amounts of fluoride during the whole period of evaluation (p>0.05). Only Vitremer® released significant amounts of fluoride ions during the 15 days of the experiment, with greater release in first 2 days (p<0.05) and stabilization in the subsequent days (p>0.05). In conclusion, the nanohybrid composite resin Tetric® N-Ceram did not present in vitro fluoride-releasing capacity throughout the 15 days of study.
University Library System, University of Pittsburgh
2015-02-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
laboratory research
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/29
10.5195/d3000.2015.29
Dentistry 3000; Vol. 3 No. 1 (2015); 9-12
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/29/28
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/30
2017-01-06T19:38:05Z
dentistry3000:MoOD
Chronic periodontitis with familial aggregation and discordant identical twins
Grech, Sarah Christina
Genetics
Chronic periodontitis is an advancement of gingivitis that involves progressive attachment loss, pocketing, and bone loss around the teeth. It has been linked to both genetic and environmental factors, making it a multifactorial disease. This case examines a family of eight siblings with a 50% prevalence of chronic periodontitis. A strong genetic influence is not likely, since one identical twin in this family has chronic periodontitis, while the other twin is unaffected. Environmental risk factors, including smoking, emotional stress, and poor oral hygiene, are present in all affected individuals, thus appearing to play a significant role in the development of periodontitis in this family. It is important for a dentist to understand the etiology of the family’s chronic periodontitis in order to provide proper treatment and management of risk factors so that good oral health can be maintained.
University Library System, University of Pittsburgh
2015-04-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Segregation Analysis
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/30
10.5195/d3000.2015.30
Dentistry 3000; Vol. 3 No. 1 (2015); 44-46
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/30/29
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/31
2017-01-06T19:38:07Z
dentistry3000:A%26tE
Familial aggregation of phenotypic expression of premature hair hypopigmentation in the craniofacial region
Black, Corey
Premature hypopigmentation
gray hair
hair color
male
tobacco
genetic
shaft
follicle
human
adolescent
melanocyte
melanin
stem cells
There are many patients who experience premature hypopigmentation of hair, but do not understand the underlying causes and potential dangers associated with them. The causes range from genetic predisposition to environmental influences such as tobacco use. Premature hypopigmentation of the hair shaft can also be associated with many syndromes; some which cause dental anomalies. Today, treatment options are limited for patients, although various studies are being done on mice to target the underlying mechanism of action. Understanding the differences between all of the possible causes of this particular phenotype can help clinicians better identify the symptoms, educate patients, and possibly modify treatment to suit the needs of each patient on an individual basis.
University Library System, University of Pittsburgh
2015-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/31
10.5195/d3000.2015.31
Dentistry 3000; Vol. 3 No. 1 (2015); 2-4
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/31/31
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/32
2017-01-06T19:38:08Z
dentistry3000:I%26A
Familial aggregation of mandibular prognathism
Lee, April
Yang, Chris
class III malocclusion
orthognathic surgery
eastern Asian
Mandibular prognathism is a hereditary condition where there is an excess growth of the mandible in relation to the maxilla that can be associated with maxillary retrusion, mandibular protrusion, or both. Skeletal mandibular prognathism is most prevalent in Eastern Asian populations. This paper focuses on a Korean family with skeletal mandibular prognathism that was inherited through three generations. Apparently, neither mandible nor maxilla is retruded in the affected individuals, but there is a concave facial profile. The dentition has a class I occlusion with skeletal mandibular prognathism, and the only way to treat this case would be orthognathic surgery with the help of orthodontic appliances.
University Library System, University of Pittsburgh
2015-04-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/32
10.5195/d3000.2015.32
Dentistry 3000; Vol. 3 No. 1 (2015); 13-15
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/32/30
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/33
2017-01-06T19:38:09Z
dentistry3000:I%26A
Agenesis of all third molars in two half siblings
Kaufer, Jamie A
PAX9
tooth agenesis
Tooth agenesis is one of the most common dental anomalies and is influenced by factors including patient genetics. Although there are several specific genes associated with certain patterns of agenesis, there does not seem to be a genetic pattern identified to date that is associated with isolated complete third molar agenesis. This report presents two half-siblings who both express complete agenesis of third molars despite the fact that their shared parent does not express the same phenotype. The case discussion focuses on addressing the potential genetic possibilities including autosomal dominant inheritance and variable expressivity of a mutation. There remains an uncertainty to the exact gene involved. Potential options include WNT10A and PAX9. The affected individuals are not needing extractions or experiencing complications due to the presence of third molars. However, the concern remains that there is the potential for more severe expression of the mutation in future generations.
University Library System, University of Pittsburgh
2015-04-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Family segregation analysis
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/33
10.5195/d3000.2015.33
Dentistry 3000; Vol. 3 No. 1 (2015); 16-18
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/33/32
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/34
2017-01-06T19:38:11Z
dentistry3000:A%26tE
Familial aggregation of anxiety associated with bruxism
Gorski, David
Bruxism
Anxiety
Genetics
Heritability
Background: This paper reports the co-occurrence of heritable anxiety-related disorders and awake bruxism in a family and discusses the heritability of anxiety and implications of awake bruxism as comorbidity to anxiety disorders.Subjects: Ten out of 14 members of an extended family reported having a professionally diagnosed anxiety-related disorder. All individuals with anxiety disorders also showed intraoral signs of wear from bruxism and reported being aware of grinding their teeth while awake. Additionally, three out of the four family members without an anxiety-related disorder did not report grinding their teeth and showed no occlusal wear from bruxism. All of the individuals that were examined were educated about the short-term and long-term complications associated with bruxism, but all of them elected to not have treatment performed.Practical Implications: Anxiety disorders are highly related to suicidal behaviors, particularly in children and adolescents. Additionally, awaken bruxism can often serve as an indicator of anxiety or stress. By recognizing bruxism as a possible manifestation of psychological distress, the dental practitioner may be able to direct patients to life-saving services like psychologists and crisis hotlines when appropriate.
University Library System, University of Pittsburgh
2015-07-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/34
10.5195/d3000.2015.34
Dentistry 3000; Vol. 3 No. 1 (2015); 5-8
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/34/33
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/36
2017-01-06T19:38:12Z
dentistry3000:DoCS
Pilot study of pre-maxilla replacement and bone graft in a patient with lip and palate clefts: transforaminal relationship between upper canines and bone graft
Carlini, João Luis
Del Santo, Danielle
Asami, Kesia Keiko
lip and palate cleft
bone graft
upper canines
dental eruption
pre-maxilla
Objective: The aim of this study was to assess patients subjected to replacement surgery of the pre maxilla with bone graft, having as donor area the iliac crest and chin, and the relation with upper canines erupted in grafted areas. Materials and Methods: 54 records were evaluated from cleft patients that were under treatment at CAIF - Center of Attention to Clefts in Curitiba, PR, Brazil, both sexes, with unilateral and bilateral lip and palate clefts. Patients younger than fourteen years old, both sexes, that lost the surgical segment or did not continue the treatment at same welcoming institution, were excluded. The surgical segment average was 5.72 years. Results: Using both descriptive and chi-square statistics, we observed a statistically significant distinction regarding the eruption of upper canines in the region of secondary bone grafts when using iliac crest and chin grafts, which 62.8% of the sample that was treated with chin grafts happened to have an eruption of upper canines, and 37.2% were successful with iliac crest grafts (value-p 0.028). Conclusions: It is necessary to carry out a study with a larger sample size and where the samples are paired in order to confirm the superiority the iliac crest donor area as the gold standard for this surgical procedure.
University Library System, University of Pittsburgh
2015-09-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Retrospective Observational Study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/36
10.5195/d3000.2015.36
Dentistry 3000; Vol. 3 No. 1 (2015); 24-31
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/36/34
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/37
2017-01-06T19:38:14Z
dentistry3000:DoCS
Modification of the technique of osteotomy for surgical maxillary expansion
Carlini, João Luis
Rieth, Cristina
Severo, Danielle Del Santo
Asami, Kesia Keiko
Osteotomy
atresia
maxillary
expansion
Surgically assisted maxillary expansion is a technique used to correct transverse maxillary deficiency, which is a dentofacial anomaly related to the decrease of the upper arch over the lower arch. It is applied to patients in their late teens and adults due to skeletal maturity, causing obliteration of the intermaxillary suture, which requires orthodontic procedure, associated with surgery. The purpose of this study is to report the clinical case of a patient admitted for surgical maxillary expansion through the modified technique and point out the possible advantages, such as aesthetics maintenence, long-term stability, faster return to orthodontic treatment, and improved bone healing. However, there are some contraindications when the patient presents severe crowding, roots of the canine and lateral incisor are converging and in patients who will undergo to protraction with facial mask.
University Library System, University of Pittsburgh
2015-10-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Report
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/37
10.5195/d3000.2015.37
Dentistry 3000; Vol. 3 No. 1 (2015); 38-43
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/37/35
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/38
2017-01-06T19:38:15Z
dentistry3000:DoCS
Tooth malposition in patients with cleft lip and/or palate in a Brazilian population
Melo Filho, Mario Rodrigues
Dias, Verônica Oliveira
Martelli, Daniella Reis
Paranaíba, Lívia Ribeiro
Swerts, Mário Sérgio Oliveira
de Barros, Letízia Monteiro
Martelli Júnior, Hercílio
Teeth abnormalities
cleft lip
cleft palate.
The purpose of the present study was to evaluate the prevalence of teeth malposition (rotated) in Brazilian patients with oral clefts and to contribute to the definition of subphenotypes. This study included 317 patients with nonsyndromic cleft lip with or without cleft palate. Tooth malposition was assessed clinically, through radiographs, and medical history records for each individual. Only teeth malpositions outside the area of the clefts were included. Comparisons were assessed by cross-tabulation and standard chi-square test, and statistical significance was set at p≤0.05. Cleft lip and palate was more prevalent in males, while cleft palate was more common in females. Regarding the presence of tooth malposition, of the 317 patients, 92 (29.02%) had at least one tooth with the dental anomaly. Tooth malposition was more common in patients with cleft lip and palate (16.1%), followed by, respectively, cleft palate (6.9%) and cleft lip (6%) (p=0.112). The highest occurrence of tooth malposition was in the mandible and involved the canines (p<0.01). Few studies have investigated the prevalence of tooth malposition in individuals with nonsyndromic cleft lip with or without cleft palate. Our results confirmed the highest occurrence of dental anomalies, particularly tooth malposition, in patients with oral clefts. Our findings also highlight that there was a higher occurrence of this condition in the mandible and not the maxilla.
University Library System, University of Pittsburgh
2015-10-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Epidemiological studies
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/38
10.5195/d3000.2015.38
Dentistry 3000; Vol. 3 No. 1 (2015); 32-37
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/38/37
Copyright (c) 2015 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/39
2017-01-06T19:38:17Z
dentistry3000:EDT
Rescuing Case Reports
Vieira, Alexandre Rezende
University Library System, University of Pittsburgh
2018-12-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/39
10.5195/d3000.2015.39
Dentistry 3000; Vol. 3 No. 1 (2015); 1
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/39/39
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/40
2017-01-06T19:38:18Z
dentistry3000:I%26A
Haitian orphan population and protective factors against caries
Rea, Madelyn
Dental Caries
Enamel Discoloration
Dental Trauma
Objective In Haiti, families were torn apart and children were left orphans after the 2010 earthquake. In the aftermath of this natural disaster many children were relocated to orphanages across the country and adopted internationally. Years later these children find themselves catching up in growth physically, mentally and emotionally after an extremely traumatic event during a crucial time in their health development. Another important marker of development is the primary dentition and the presence of caries. We report estimates of early childhood caries (ECC) frequency, risk factors and quality of health among Haitian children. Methods Medical and dental professionals conducted a descriptive cross sectional study through the Pittsburgh Kids Foundation and their partnership with IDADEE children’s home, EBAC orphanage and New Vision Children’s home. Vital signs were taken and recorded to create a health/growth history for each child. Brief dental screenings were conducted and topical fluoride treatments were administered. Risk factors and quality of health information was obtained from discussions with the caregivers present. The children and caregivers were given oral hygiene education and supplies (i.e. toothbrushes, toothpaste, floss). Results Physical exams and dental screenings were conducted on the 40 children ages 3-10 years of age living in the IDADEE children’s home. Two children had cavitated teeth. Eight children had teeth that were stained. Four children had evidence of dental trauma. 26 out of the 40 children had otherwise healthy dentition. Conclusion The IDADEE children’s home and New Vision Children’s home have hopes to expand their capacity with new construction scheduled to be finished in 2016. As more children enter these homes action is needed to educate caregivers on ways to identify high-risk children to prevent ECC and ways they can be treated before irreversible damage is done to the developing permanent dentition.
University Library System, University of Pittsburgh
2016-04-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/40
10.5195/d3000.2015.40
Dentistry 3000; Vol. 3 No. 1 (2015); 19-23
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/40/42
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/47
2017-04-13T14:25:17Z
dentistry3000:A%26tE
Orthodontic treatment as a possible trigger of periodontal disease
Lin, Ann
Periodontal disease
orthodontic treatment
attachment loss
family history
stress
Background: Periodontal disease leads to the deterioration of oral health, including loss of teeth and its supporting structures. It has been established that the cause of this disease is multifactorial, indicating that both genes and environmental factors are intertwined with the formation and progression of periodontal disease. Case Description: Here we discuss the possibility of orthodontic treatment serving as a trigger to an early development of periodontal disease in a patient who has a family history of periodontitis. Between debonding at age 17.5 years and age 28, the patient experienced gradual general loss of attachment on the mandibular arch with the left mandibular canine having exaggerated attachment loss. Between age 28 and 29 years, the patient experienced exacerbated loss of attachment at both mandibular canines. Practical Implications: The occurrence of attachment loss during and after orthodontic treatment should not be overlooked. It is crucial for orthodontists to obtain a family history of periodontal disease and carefully monitor patient’s periodontal conditions throughout the treatment.
University Library System, University of Pittsburgh
2016-06-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/47
10.5195/d3000.2016.47
Dentistry 3000; Vol. 4 No. 1 (2016); 2-5
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/47/43
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/49
2017-04-13T14:25:17Z
dentistry3000:I%26A
Third Molar Eruption Mechanisms and Patterns
Zhang, Winnie
Third molars
etiology
impacted
abnormality
hereditary
Third molars are highly variable in their presence and form. This report focuses on a horizontally impacted third molar and analyzes the potential etiology of this situation. Upon a clinical and radiographic examination, it was noted that the patient had four third molars present. The patient’s third molars began erupting around the age of 19. Currently, they are asymptomatic with incipient caries on the occlusal surfaces. While three of the third molars erupted in a normal orientation, one of the third molars (mandibular left) erupted in an orientation that would be classified as horizontal and/or mesio-angular. No treatment has been undertaken at the moment, however surgical extraction can be recommended with the prognosis being very good. For the clinician that has to treat dental complications that arise from abnormal tooth eruption, as seen in numerous genetic and acquired disorders, knowledge about the basic molecular mechanisms involved may become extremely important.
University Library System, University of Pittsburgh
2016-09-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/49
10.5195/d3000.2016.49
Dentistry 3000; Vol. 4 No. 1 (2016); 11-16
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/49/44
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/52
2017-04-13T14:25:17Z
dentistry3000:A%26tE
Observing Anterior Crowding In a Population Living a Century Ago
Cetnar, Christopher
Purpose: To observe the frequency of mandibular anterior crowding in a population who lived during the nineteenth century and compare the rate of occurrence in the current population, which is claimed to be two-thirds of adults experiencing crowding by early adulthood. Materials and Methods: The School of Dental Medicine at the University of Pittsburgh possesses a collection of skulls of individual’s likely living in the northeast United States during the late 19th century and early 20th century. Of this skull collection, 29 skulls were evaluated for the presence of mandibular anterior crowding. Results: 55% of the evaluated mandibular anterior arches were positive for mandibular anterior crowding. 57% of the male specimens were positive for anterior crowding, while 50% of the females were recorded to have anterior crowding. These frequencies are lower than the one suggested for the current live population in the United States. Conclusion: From this sample of the population living a century ago, there was a lower prevalence of anterior crowding (55%) than the research suggested two-thirds of adults.
University Library System, University of Pittsburgh
2016-09-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/52
10.5195/d3000.2016.52
Dentistry 3000; Vol. 4 No. 1 (2016); 6-10
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/52/45
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/55
2017-04-13T14:25:17Z
dentistry3000:DoCS
Prevalence of oral tori among medical and dental Students at the University of the West Indies
Rajendra Santosh, Arvind Babu
Jones, Thaon
Venugopal, Hima
Smith, Keisha
Turpin Mair, J Suzanne
Edwards, Mark
Williams, Errol
Torus palatinus
torus mandibularis
oral tori
exososes
Jamaica
prevalence
Objective: To determine the prevalence and gender variation of torus palatinus (TP) and torus mandibularis (TM) amongst medical and dental students at the Mona campus of the University of the West Indies. Subjects and Methods: The study observed 335 medical and dental students from the Mona campus of the University of the West Indies. Routine dental examinations were conducted to check the presence/absence of TP and TM by inspection and palpation. Results: The overall prevalence of TP and TM was 27.76%. The oral torus prevalence was as follows, torus palatinus was 44.08%, torus mandibularis was 36.55% and individuals with both torus palatinus and mandibularis was 19.35%. The study observed that the prevalence of TP and TM in females was 16.11% (54/335), and males 11.64% (39/335). It was also found that bilateral torus mandibularis (40.38%) was more prevalent than unilateral right torus mandibularis (36.53%) and unilateral left torus mandibularis (23.07%). Conclusions: The prevalence of TP and TM are comparatively higher than neighboring Caribbean nations and West African countries. Our observations also highlighted that TP is highly prevalent among various types of oral tori. The study also recorded higher prevalence of oral tori among females. Due to higher prevalence of oral tori among the study group, Jamaican dentists need be knowledgeable about the high prevalence and clinical significance of TP and TM for dental treatment.
University Library System, University of Pittsburgh
2016-10-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Control Study; Epidemiology
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/55
10.5195/d3000.2016.55
Dentistry 3000; Vol. 4 No. 1 (2016); 25-33
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/55/46
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/56
2017-04-13T14:25:17Z
dentistry3000:I%26A
The effect of polymer burs on microbiological reduction of carious dentin in deciduous teeth: a systematic review
Oliviera, Daniela Silva Barroso de
Barreiros, Driely
Silva, Léa Assed Bezerra da
Silva, Raquel Assed Bezerra da
Nelson-Filho, Paulo
Kuchler, Erika Calvano
polymer burs
dental caries
Streptococcus mutans
Lactobacillus mutans removal
Background. Polymer bur is a new technology that proposes to conserve the dentin that is capable of remineralization. Aim: To conduct a quantitative systematic review to evaluate the effect of polymer burs on the reduction of Streptococcus mutans and Lactobacillus after dentin carious excavation in deciduous teeth. Methods and Material: Two reviewers performed the database to identify the relevant clinical papers. Only papers in English that compared polymer burs with other caries removal techniques with the microbiological aspects as an outcome were included. Papers that evaluated only permanent teeth were excluded. Risk of bias was also assessed. Individual study effect sizes were calculated using Cohen’s d formula for the comparisons of microorganism reduction before and after carious excavation. Results: The search resulted in 12 non-duplicated papers. After the revision, only 2 were selected. The quantitative evaluation demonstrated that polymer bur reduces microorganism levels in carious dentin. The larger effect size observed was for Streptococcus mutans in the polymer bur technique (r=0.84; d=3.12), followed by Lactobacillus in the carbide bur technique (r=0.83; d=3.03). Conclusion: Polymer burs promoted a significant reduction of microorganism levels, mainly Streptococcus mutans in carious dentin.
University Library System, University of Pittsburgh
2016-10-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Systematic Review
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/56
10.5195/d3000.2016.56
Dentistry 3000; Vol. 4 No. 1 (2016); 17-24
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/56/50
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/57
2017-04-13T14:25:17Z
dentistry3000:DoCS
Association of AXIN2 gene polymorphisms with nonsyndromic oligodontia in Turkish families
Dinckan, Nuriye
Uyguner, Zehra Oya
Kayserili, Hulya
Letra, Ariadne
AXIN2
WNT
tooth agenesis
oligodontia
association
Tooth agenesis is the most common developmental abnormality of the human dentition characterized by the congenital absence of one or more permanent teeth. Oligodontia is the term used to describe severe tooth agenesis, where six or more permanent teeth are missing. The WNT gene pathway regulates multiple developmental processes during craniofacial and tooth development, and variations in WNT pathway genes have been reported in individuals with tooth agenesis. In this study, we investigated the association of 37 SNPs in/nearby 12 WNT pathway genes (WNT3, WNT3A, WNT5A, WNT8A, WNT9B, WNT10A, WNT11, AXIN1, AXIN2, APC, LRP5, LRP6) with oligodontia in 22 multiplex families. Genotypes were generated using Taqman chemistry in a real-time polymerase chain reaction assay. Family-based association tests were performed using FBAT. Pairwise-haplotype analysis was also performed. Bonferroni correction was used to adjust for multiple testing and P-values ≤ 0.001 were considered statistically significant. We found nominal association for AXIN2 rs7591, located in the 3’ UTR, with oligodontia (P=0.04). In silico analysis of SNP function predicted a binding site for miR-205 with potential impact on AXIN2 expression. Although modest, these results continue to support a role for AXIN2 in the etiology of familial tooth agenesis.
University Library System, University of Pittsburgh
2016-10-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/57
10.5195/d3000.2016.57
Dentistry 3000; Vol. 4 No. 1 (2016); 34-42
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/57/51
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/58
2017-04-13T14:25:17Z
dentistry3000:MoOD
Multilevel modeling for dental caries among adolescents in a Brazilian large city
Pecharki, Giovana Daniela
Brancher, João Armando
Olandoski, Márcia
Doetzer, Andrea Duarte
Moyses, Samuel Jorge
Trevilatto, Paula Cristina
Oral health
Risk indicators
Caries Experience
Dental caries is a complex disease, which needs an approach that considers caries influencing factors at different levels and their integration. Multilevel Modeling is a clustered analysis of variables from the individual to the community level. The aim of this study was to investigate the association of social and biological factors grouped into hierarchical levels, in students with caries. A sample of 687 students was evaluated from public and private schools of Curitiba. The parameters evaluated were: individual level, school level and district level. Individual variables had a highly significant association with caries experience, also in the presence of school and district levels. Male sex negatively associated with caries experience. However, the interaction between male sex and no fluoride use was positively associated with caries. Lower socioeconomic status, dental biofilm, and fluorosis were associated with caries. Nevertheless, the interaction between dental biofilm and fluorosis was negatively associated with caries experience. The interaction between no flossing and use of public dental services were also associated with caries outcome. Individual factors were associated with caries experience even with the inclusion of contextual variables in the study population.
University Library System, University of Pittsburgh
2016-10-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Multilevel modeling
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/58
10.5195/d3000.2016.58
Dentistry 3000; Vol. 4 No. 1 (2016); 43-54
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/58/53
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/59
2018-05-30T12:38:38Z
dentistry3000:A%26tE
Oral mucosal lesions in patients with pemphigus and pemphigoid skin diseases: a cross sectional study from southern India.
Rajendra Santosh, Arvind Babu
Reddy Baddam, Venkat Ramana
Anuradha, Chigurupati
Poosarla, Chandrashekar
Pemphigus
pemphigoid
autoimmune
epidemiology
oral disease.
Objective:To assess the prevalence of oral mucosal lesions in patients with pemphigus and pemphigoid diseases from Southern India. Design and Methods: A cross-sectional hospital based study was conducted from August 2010 to July 2011. Patients with confirmed pemphigus and pemphigoid skin disease were selected and informed to participate in the study. Oral examination of all participants were done to document site and type of oral manifestation and diagnostic procedures such as histopathological and Immunofluorescence methods were performed to confirm the diagnosis. Demographic details such as age, gender and occupation were also documented. The results of the study were analyzed by SPSS software version 19.0 (Armonk, NY) and presented as descriptive statistics. Results: Sixty percent of the patients exhibited oral mucosal manifestations. A higher female (86.66%) predilection of autoimmune blistering disease was observed in the study. A slightly higher number of pemphigoid patients (53.33%, 16 out of 30) were reported than pemphigus (46.66%, 14 out of 30 cases). The most common subtypes of pemphigus is Pemphigus vulgaris 71% (10 out of 14) among pemphigus, and bullous pemphigoid 87.5%(14 out of 16) among pemphigoid. Buccal mucosa (92.85%) is the most common site in pemphigus patients, where as hard palate (12.5%) is mostly commonly reported site in pemphigoid patients. Initial involvement of oral tissue in disease process was observed in 78.57% of pemphigus, and 12.5% of pemphigoid patients. Conclusion: Oral mucosal lesions are more frequently associated in pemphigus patients. Oral mucosal lesions are the initial site of disease process in pemphigus patients. The significance of diagnosis of oral lesions at earlier stage of disease, specifically pemphigus may help in early intervention of disease and help to reduce the morbidity and mortality state. The study emphasizes multidisciplinary approach in diagnosis and management of both pemphigus and pemphigoid.
University Library System, University of Pittsburgh
2017-03-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Epidemiology - Cross sectional
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/59
10.5195/d3000.2017.59
Dentistry 3000; Vol 5, No 1 (2017); 2-8
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/59/56
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/60
2019-07-25T12:14:07Z
dentistry3000:A%26tE
Assessment of knowledge among public and private elementary school teachers in dental trauma management
Bayram, Merve
Koruyucu, Mine
Seymen, Figen
Purpose: Traumatic dental injuries, majority of which occur at school and primary care given by teacher, should managed as soon as possible. The knowledge and attitudes of teachers is critical for the best prognosis of traumatized teeth. The purpose of this study was to evaluate knowledge and attitudes among elementary public and private school teachers in dental trauma management.Materials and Methods: A modified three-part questionnaire comprised of questions regarding demographic data, attitude and knowledge about dental trauma was distributed to 328 teachers (public schools:164/private schools:164). The results of the questionnaire were expressed as frequency distributions. Statistical analysis was performed by version 20.0 of the SPSS statistics software.Results: The average correct knowledge score was 4.96 out of 10. Two individual predictors significantly improved the respondents’ knowledge: being more than 50 years old age (p=0.001) and more than 15 years teaching experience group (p<0.001). While 84.5% of public, 79% of private school teachers found their knowledge poor; and 85% of public, 76% of private school teachers state they're not satisfied with their level of knowledge. Private school teachers had given significantly more correct answers to the questions about dental avulsion management than public school teachers (p=0.01). There was no significant difference between public and private school teachers' knowledge regarding dental trauma management.Conclusion: The level of knowledge in dental trauma management determined in this study was unsatisfying. Programs on increasing the awareness and motivation of teachers on dental trauma management in both types of schools are recommended.
University Library System, University of Pittsburgh
2017-06-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/60
10.5195/d3000.2017.60
Dentistry 3000; Vol 5, No 1 (2017); 9-15
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/60/60
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/65
2017-04-13T14:25:17Z
dentistry3000:EDT
The Issue of Peer-Review
Vieira, Alexandre Rezende
University Library System, University of Pittsburgh
2016-12-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/65
10.5195/d3000.2016.65
Dentistry 3000; Vol. 4 No. 1 (2016); 1
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/65/54
Copyright (c) 2016 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/66
2019-07-25T12:14:07Z
dentistry3000:MoOD
Could recurrent aphthous stomatitis be linked to cancer development?
Norton, Emily
recurrent aphthous stomatitis
cancer
TNF-alpha
Background: Recurrent aphthous stomatitis is a common multifactorial oral mucosal disorder. Genetic and local factors causing RAS may contribute to the development of cancer. This study aims to explore this connection by examining a family with a history of RAS and ovarian cancer. Case Description: The family described in the case exhibits a history of minor recurrent aphthous stomatitis. The severity of this condition increases with each generation. One member of the family has no history of RAS but was diagnosed with ovarian cancer. Genetic testing for BRCA mutations was negative indicating a different genetic cause of the cancer. All affected members of the family indicate high levels of stress or difficulty responding to stressful situations. No treatment for RAS was performed due to its self-limiting nature. Practical Implications: Further research is needed before dentists begin to tell their patients with RAS they may be at higher risk of developing cancer. Diligent oral cancer screening and stress management counseling can decrease the risk to the patient.
University Library System, University of Pittsburgh
2017-06-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/66
10.5195/d3000.2017.66
Dentistry 3000; Vol 5, No 1 (2017); 45-49
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/66/61
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/68
2019-07-25T12:14:07Z
dentistry3000:MoOD
A case of molar-incisor hypomineralization with genetic and environmental influences
Kello, Erin
MIH
Molar-Incisor Hypomineralization
genetics
enamel development
case study
environmental risk factors
Background: Molar-Incisor Hypomineralization (MIH) is a common childhood dental pathology. This paper describes a case with familial involvement and environmental risk factors. Case Description: A 35-year old female has yellowish-brown opacities present on central and lateral maxillary incisors, right and left maxillary canines, right and left maxillary first molars, and right and left maxillary second molars. A 33-year old male sibling has yellowish-brown opacities on the right and left central and lateral maxillary incisors. The male sibling’s fraternal twin does not have evidence of MIH. A maternal grandmother also had evidence of MIH, though involved teeth are not known. Practical Implications: MIH is a condition with both genetic and environmental components. Practitioners should consider both etiologies when patients present with the condition.
University Library System, University of Pittsburgh
2017-06-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/68
10.5195/d3000.2017.68
Dentistry 3000; Vol 5, No 1 (2017); 50-53
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/68/62
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/69
2019-07-25T12:14:07Z
dentistry3000:A%26tE
Potential inheritance patterns of a prothrombin gene mutation in a 23-year-old female and ethical considerations of a positive diagnosis: a case report
Brand, Hannah
Prothrombin
mutation
hereditary hemophilia
clotting factor
bleeding disorder
Background: Prothrombin, also called Factor II, is a blood clotting protein found in all individuals that is necessary to form blood clots. In most individuals, a balance between bleeding and blood clot formation occurs. However, in individuals with a mutation in the prothrombin gene, the balance is disrupted due to excess production of prothrombin which leads to an increase in blood clot formation (1). Inherited predispositions to blood clot formation are termed hereditary thrombophilia. Prothrombin G20210A mutations are one of the most common hereditary gene associations. Case Description: This report examines the case of a 23-year-old female who has tested positive for the prothrombin gene mutation. The individual has an extensive history of blood clots including 8 deep vein thromboses (DVTs), 4 pulmonary embolisms with one complete infarction, 4 superficial clots, and a miscarried pregnancy attributed to her thrombophilia. The individual has a significant family history of the mutation and takes Coumadin daily for prevention of further clots. Practical and Ethical Implications: Despite a strong familial history of blood clots and related hospitalizations, the parents of the case individual do not want to get their other children tested for the mutation for fear that the children will be denied insurance coverage in their futures. The case individual will likely continue to be on blood thinners indefinitely. This will affect many aspects of her life, including dental treatment, as she will be at an increased risk for bleeding.
University Library System, University of Pittsburgh
2017-06-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/69
10.5195/d3000.2017.69
Dentistry 3000; Vol 5, No 1 (2017); 16-19
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/69/63
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/70
2019-07-25T12:14:07Z
dentistry3000:MoOD
Etiology and familial inheritance of pleomorphic adenomas
Clark, Krysten
Pleomorphic Adenoma
Carcinoma ex Pleomorphic Adenoma
PLAG1
Background: A pleomorphic adenoma is the most common salivary gland neoplasm in both children and adults. Pleomorphic adenomas are derived from ductal and myoepithelial cells and are most commonly found in the superficial lobe of the parotid gland. The purpose of this article is to discuss the genes involved in pleomorphic adenomas and the possible autosomal dominant mode of inheritance. Case Description: The first patient was a white male who was diagnosed with carcinoma ex pleomorphic adenoma, a highly aggressive tumor, at the age of 57. He had an undiagnosed pleomorphic adenoma for approximately 15 years prior. The tumor was excised and the patient underwent radiation in the location of his parotid gland for 4 years until he deceased. The second patient is a white female, his daughter, who was diagnosed with a benign pleomorphic adenoma at the age of 46. Her salivary gland tumor was excised and normal follow up appointments occurred. Practical Implications: Pleomorphic adenomas most commonly affect the parotid gland, the largest of the three major salivary gland tumors. Occurrence and excision of this salivary gland tumor will cause a decrease in the secretion of saliva, leading to a dry mouth and an increased risk of caries.
University Library System, University of Pittsburgh
2017-06-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/70
10.5195/d3000.2017.70
Dentistry 3000; Vol 5, No 1 (2017); 54-58
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/70/64
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/73
2019-07-25T12:14:07Z
dentistry3000:I%26A
Behavioral genetics and thumb sucking in adolescents
Hatala, Jessica
Nonnutritive sucking
thumb sucking
behavioral genetics
malocclusion
Thumb sucking is a common habit developed by children and begins as early as in utero. However, it becomes problematic when a child continues to suck their thumb past the age of 4 years, when their secondary dentition is developing and preparing to erupt. Prolonged thumb sucking into adolescence can have deleterious effects on dental and skeletal structures based on the duration and how frequent the child engages in this nonnutritive sucking habit. Thumb sucking can lead to various types of effects such as increased overjet, anterior open bite, posterior cross bite, maxillary arch constriction, high palatal vault, and Class II malocclusion. This paper presents a case study, which focuses on chronic thumb sucking in a family, the dental and skeletal changes that the family members experienced, and whether or not this prolonged behavior can be genetically influenced. Based on this case study and the field of behavioral genetics it is possible that there can be a genetic component to the duration of thumb sucking into adolescence.
University Library System, University of Pittsburgh
2017-06-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/73
10.5195/d3000.2017.73
Dentistry 3000; Vol 5, No 1 (2017); 20-24
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/73/71
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/74
2019-07-25T12:14:07Z
dentistry3000:MoOD
Oral candidiasis: relation to systemic diseases and medications
Chu, Xiaozhu
oral candidiasis
disease
medication
xerostomia
Background: Oral candidiasis is by far the most common oral fungal infection in humans. It is caused by the fungal organism Candida albicans. When the host is debilitated by other diseases and conditions, C. albicans, which is usually a part of the normal oral flora, can turn pathogenic and invade the host tissue to cause the infection. The purpose of this paper is to investigate the role of systemic diseases and medications in the development of oral candidiasis.Methods: A total of 12 cases with code indicating oral candidiasis were collected from the University of Pittsburgh School of Dental Medicine Dental Registry and DNA Repository. The systemic diseases and medications were descriptively analyzed.Results: 50% of the subjects had more than two systemic diseases. The most prevalent diseases were mental illnesses (50%), cardiovascular diseases (41.7%), and respiratory system diseases (33.3%). 50% of subjects were on polypharmacy therapies and 75% of subjects were taking medications that may contribute to oral candidiasis. Among the medications, antidepressants and inhalational corticosteroids may have strong potentials to cause oral candidiasis.Conclusion: Oral candidiasis is associated with having systemic diseases and intake of medication, especially with those medications can cause xerostomia. As the number of systemic diseases and medications increases, the risk of developing oral candidiasis may increase too.
University Library System, University of Pittsburgh
2017-06-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/74
10.5195/d3000.2017.74
Dentistry 3000; Vol 5, No 1 (2017); 59-64
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/74/66
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/75
2019-07-25T12:14:07Z
dentistry3000:MoOD
Familial inheritance of TMD: a case report
Genello, Maria
Familial TMD
systemic joint laxity
chronic headaches/pain
craniofacial dimensions
TMD inheritance
The TMJ is a joint that can perform both hinge and sliding motions and whose proper functioning depends on the actions of the joint and joint capsule, the muscles of mastication, and the condition and action of the mandibular condyle. If any of these components deviate from their normal structure or function, then TMD can result. There have been many reported contributing factors to TMD development, many of which include environmental influences such as bruxism, trauma, and other oral habits. However, a genetic component can also play a role. This paper reports a case of TMD occurring within three consecutive generations of a family and explores the possibility of this disorder exhibiting familial inheritance. Members of the family affected all displayed manifestations of joint laxity in other areas of the body, migraines, pain, and had smaller craniofacial dimensions with a narrower mandible. These factors were found to have a genetic influence and these genes can also be tied to TMD, thus supporting the argument that the cases of TMD seen in this family are in fact due to inheritance. If TMD can be shown to have a genetic component and be inherited, then dental practitioners would be able to identify high risk patients and help to modify environmental factors early on in order to help prevent the onset of TMD in those individuals.
University Library System, University of Pittsburgh
2017-06-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/75
10.5195/d3000.2017.75
Dentistry 3000; Vol 5, No 1 (2017); 65-69
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/75/68
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/76
2019-07-25T12:14:07Z
dentistry3000:DoCS
Assessment of dentofacial variations in monozygotic twins
Tu, Victor
With dentofacial development being a multifactorial process, we face the question of whether genetic or environmental factors play a larger role in specific phenotypic traits. The use of twin studies allows one to employ genetic controls to the study such that one can focus solely on traits that may be more environmentally determined. This study involves retrospective as well as current analytical measurements between a set of monozygotic twins to assess genetic and outside influences on facial and intraoral development. While similarities were acknowledged, we found differences in measurements of the facial thirds, the congenital presence of mandibular third molars, and the presence of mandibular tori.
University Library System, University of Pittsburgh
2017-06-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/76
10.5195/d3000.2017.76
Dentistry 3000; Vol 5, No 1 (2017); 25-29
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/76/65
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/77
2019-07-25T12:14:07Z
dentistry3000:DoCS
Class III malocclusion: an argument for early orthodontic treatment
Moe, Eric
malocclusion
Angle Class III
orthodontics
genetics
craniofacial
patient education
Class III malocclusion is a complex multifactorial condition with many genetic and environmental influences. Most often the condition is inherited in a Mendelian autosomal dominant pattern. Early referral and treatment can lead to better outcomes in orthodontic therapy. The subject presented for an early orthodontic referral at age 5.5 and showed signs of future Class III malocclusion. A rapid maxillary expander was given as interventional treatment for one year. At age 11.5 the subject was treated with braces for 2 years. The outcome was a normal Class I occlusion. The subject’s mother had Class III malocclusion but was not evaluated early and was only able to establish an edge-to-edge Class III malocclusion as the best treatment outcome without orthognathic surgery. The subject’s grandmother was also Class III, establishing an autosomal dominance pattern of inheritance in the family. This case demonstrates the importance of the general dentist educating families about malocclusion and making orthodontic referrals as early as possible so the best treatment outcomes can be reached through orthodontic therapy.
University Library System, University of Pittsburgh
2017-06-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/77
10.5195/d3000.2017.77
Dentistry 3000; Vol 5, No 1 (2017); 30-34
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/77/69
Copyright (c) 2017 Dentistry 3000
oai:ojs.dentistry3000.pitt.edu:article/78
2019-07-25T12:14:07Z
dentistry3000:DoCS
Fluctuating dental asymmetry in rabbits with familial nonsyndromic coronal suture synostosis
Hyre, Mae A.
Weinberg, Seth M.
Cooper, Gregory M.
Gilbert, James
Siegel, Michael I.
Mooney, Mark P.
Fluctuating dental asymmetry
craniosynostosis
developmental instability
rabbits
Fluctuating dental asymmetry has been linked to conditions of unstable pre- and peri-natal development. Familial, nonsyndromic craniosynostosis disrupts early craniofacial development through localized excessive calvarial ossification leading to the premature fusion of the calvarial sutures. Such abnormal gene expression may also produce systemic stress resulting in developmental instability, thereby affecting normal trait symmetry. The present study was designed to test this hypothesis by examining fluctuating dental asymmetry in an inbred strain of rabbits with familial, nonsyndromic coronal craniosynostosis. The mesiodistal (MD) and buccolingual (BL) dimensions of the right and left maxillary first molars were measured in four groups of New Zealand white rabbits (N=176; n=40 with early-onset synostosis, n=65 with delayed-onset synostosis, n=46 in-colony, phenotypically normal rabbits, and n=25 wild-type normal controls). For each variable, raw signed asymmetry was calculated (left-right) and tested for assumptions of fluctuating asymmetry (i.e., normality and non-directionality). Any group that did not meet these assumptions was excluded from further analysis. Using a standard size-adjusted, fluctuating asymmetry index, mean fluctuating asymmetry was calculated and compared across groups with non-parametric statistics. For the MD dimension, no significant (p > 0.05) group differences in mean fluctuating asymmetry were observed among groups. In contrast, rabbits with early-onset synostosis had significantly (p < 0.05) more fluctuating asymmetry in the BL dimension compared to wild-type controls. Results demonstrate increased fluctuating dental asymmetry in rabbits with nonsyndromic, early-onset coronal suture synostosis and suggest that the molecular events producing suture synostosis locally may have also have systemic effects. Knowledge of these systemic interactions may contribute to a fuller understanding of the phenotypic spectrum observed in individuals with nonsyndromic craniosynostosis.
University Library System, University of Pittsburgh
2017-10-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Experimental
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/78
10.5195/d3000.2017.78
Dentistry 3000; Vol 5, No 1 (2017); 35-44
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/78/72
Copyright (c) 2017 Mae A. Hyre, Seth M. Weinberg, Gregory M. Cooper, James Gilbert, Michael I. Siegel, Mark P. Mooney
oai:ojs.dentistry3000.pitt.edu:article/81
2019-07-25T12:14:07Z
dentistry3000:EDT
Five years of Dentistry 3000
Vieira, Alexandre Rezende
Five years of Dentistry 3000
University Library System, University of Pittsburgh
2017-12-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/81
10.5195/d3000.2017.81
Dentistry 3000; Vol 5, No 1 (2017); 1
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/81/74
Copyright (c) 2017 Alexandre Rezende Vieira
oai:ojs.dentistry3000.pitt.edu:article/82
2020-04-15T00:48:03Z
dentistry3000:DoCS
Maximum mouth opening in healthy children and adolescents in Istanbul
Koruyucu, Mine
Tabakcilar, Derya
Seymen, Figen
Gençay, Koray
Maximal mouth opening
interincisal distance
children
mouth width
Objectives: Mouth opening capacity is often regarded as one of the important parameters for evaluating the function of the temporomandibular joint (TMJ) and masticatory muscle status. A reduced mouth opening capacity may be one of the first clinical signs of TMJ involvement. The purpose of this study was to create age related percentiles for the maximal interincisal distance (MID) of healthy children. Methods: The patients admitted for routine dental examinations to Istanbul University Faculty of Dentistry, Department of Pedodontics were included in this study. The interincisal measurements were performed with metallic calliper and also malocclusions were recorded for all children. Oneway Anova test, Tukey HDS test, Tamhane’s T2 test and Student t test were used for statistical analysis. Results: The study population comprised of 1059 (569 males, 490 females), 3-to 15-year-old (mean age 8.82±3.06) children. The mean score of maximal inter-incisal distance was found 33.24±5.54 for females; 33.32±5.71 for males. There was no statistically significant difference according to gender (p=0.815; p>0.05). The mean score of maximal inter-incisal distance was found 28.63±4.34 for 3-5 years; 33.52±4.84 for 6-11 years; 37.35±5.52 for 12-15 years children. Statistically significant differences were found between age groups (p: 0.001; p<0.01). The mean score of maximal inter-incisal distance was found 32.9±5.6 for class I; 34.92±5.51 for class II; 35.2±5.36 for class III malocclusions. Statistically significant differences were found between malocclusion groups (p:0.001; p<0.01). Conclusion: The result of this study indicated that positive relationship between the maximum mouth opening and age and malocclusion.
University Library System, University of Pittsburgh
2018-07-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/82
10.5195/d3000.2018.82
Dentistry 3000; Vol. 6 No. 1 (2018); 1-7
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/82/75
Copyright (c) 2018 Mine Koruyucu, Derya Tabakcılar, Figen Seymen, Koray Gençay
oai:ojs.dentistry3000.pitt.edu:article/83
2020-04-15T00:48:03Z
dentistry3000:MoOD
Comparison of root canal length measurement methods in primary teeth
Koruyucu, Mine
Bayram, Merve
Kasımoğlu, Yelda
Seymen, Figen
apex locator
conventional radiography
RVG
SEM
working length
Objectives: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth (in-vivo) and to compare the results with scanning electron microscopy measurements (ex-vivo). Materials and Methods: This study was conducted on 50 primary molar teeth. Standard endodontic access cavity preparations were prepared and the actual length was calculated by calibrated investigators. Working lengths were determined by using conventional radiography, RVG and apex locator in a total of 116 root canals. After the extraction of the teeth, each canal length was determined by using SEM. Data were analysed statistically by using Oneway Anova, Tukey HDS, Student t test and Bonferroni tests. Results: The mean root length measurements with conventional radiography were significantly higher than apex locator, RVG and SEM. The accuracy of apex locator and RVG were higher than conventional radiography in determining the working length in primary teeth. Conclusions: The electronic apex locators provide an acceptable level of accuracy in determining root canal length in primary teeth. Clinical Relevance: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth and,to compare the results with scanning electron microscopy measurements.
University Library System, University of Pittsburgh
2018-07-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/83
10.5195/d3000.2018.83
Dentistry 3000; Vol. 6 No. 1 (2018); 8-16
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/83/76
Copyright (c) 2018 Mine Koruyucu, Merve Bayram, Yelda Kasımoğlu, Figen Seymen
oai:ojs.dentistry3000.pitt.edu:article/84
2020-04-15T00:49:27Z
dentistry3000:DoCS
The effect of estrogen on craniofacial dimensions: a systematic review
Omori, Marjorie Ayumi
Nakane Matsumoto, Mirian Aiko
Bezerra Segato, Raquel Assed
Bezerra da Silva, Léa Assed
Nelson Filho, Paulo
Kuchler, Erika Calvano
Estrogen
Craniofacial
Growth
Sex hormones have an effect on bone metabolism. However, it remains unclear how estrogen hormone affects mandible and maxilla growth and development. The aim of this study was to perform a systematic review to evaluate if estrogen is associated with developmental alterations in the maxilla/mandible phenotype. Material and methods: A computer search of the literature was performed using: Pubmed Medline (1966 – August 2018), Google Scholar and manual searching. A combination of the terms ‘estrogens', ‘mandible’, ‘dental arch’, ‘maxilla’, ‘craniofacial’, ‘growth’ and ‘development’ was used. Studies that used animal models to evaluate the role of estrogen during growth and development on the dimensions of the maxilla and/or mandible were included. Results: 5 studies were selected to compose this systematic review. One study used zebrafish as a model. Two studies used female mice and 2 studies used female rats as a model. Two studies treated the animals with estrogen. Four studies demonstrated that estrogen has an effect on mandible dimension and 2 studies demonstrated that estrogen has an effect on maxilla. One study did find an association between estrogen deficiency and mandible/maxilla dimensions Conclusion: The current evidence suggested that both, increased and decreased levels of estrogen, have an effect on the maxilla and mandible dimensions.
University Library System, University of Pittsburgh
2019-06-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/84
10.5195/d3000.2019.84
Dentistry 3000; Vol. 7 No. 1 (2019); 21-26
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/84/79
Copyright (c) 2019 Marjorie Ayumi Omori, Mirian Aiko Nakane Matsumoto, Raquel Assed Bezerra Segato, Léa Assed Bezerra da Silva, Paulo Nelson Filho, Erika Calvano Kuchler
oai:ojs.dentistry3000.pitt.edu:article/85
2020-04-15T00:49:27Z
dentistry3000:A%26tE
Systematic literature review of emergency department physicians’ confidence to treat dental pain and the frequency with which they prescribe pain medications: Considerations for improved outcomes.
Barna, Sara
O'Donnell, Jean
Oakley, Marnie
opioids
oral surgery
emergency room
Opioid analgesics, when taken as prescribed, are effective therapeutic options that provide pain relief for moderate to severe pain. The use of opioids in the treatment of pain has been increasing in the U.S. at an alarming rate, possibly contributing to the simultaneous rise in opioid abuse. Emergency departments play a major role in managing patients who present in pain, with approximately 10% of all opioid analgesic prescriptions written in hospital emergency departments. It is estimated that dental pain patients represent between 0.3-4% of the overall patient emergency department workload. Yet, the literature suggests that many of these physicians may not have sufficient training in handling dentofacial emergencies. The goal of this study was to systematically review the available literature on the topics of: 1) emergency department physicians’ training related to treating dental pain patients, and 2) the frequency in which they prescribe opioid medications to these patients. Methods: A systematic literature review was conducted among publications from 1985-2014 in the databases PubMed, Ovid, and Science Citation Index. The following search terms were used in this systematic literature review in order to identify the available literature of interest: “opioid and dental and emergency departments,” “dental pain and drug abuse,” “ER physicians and dental pain,” “ER physicians and drug abuse,” and “dental pain and emergency departments.” Publications in any language or country were considered, as well as editorials and commentaries. Findings: A total of 769 publications were identified. Seventeen publications met the criteria for inclusion. Eight studies commented on the emergency department physicians’ perceived “lack of training” in handling dentofacial emergencies and found that the majority of this group did not feel comfortable in managing dental patients. Nine studies assessed the frequency in which physicians prescribe pain medications to dental patients. Within these 9 studies, 5 specifically reported that between 29.6% and 81% of dental patients treated, received an “opioid” or a “narcotic” upon discharge. The remaining 4 studies in this group instead used non-specific terms that included “prescription medications,” “analgesics,” “pain medicine,” and “pharmacotherapy” to describe their findings and did not particularly report opioid prescribing trends. Of those publications rejected, 9 addressed the topic of dental pain patients presenting to non-dental providers, but did not include data that met the criteria related to emergency department prescribing frequency or physician training. Conclusions: This review of the literature suggests that emergency department physicians’ training level in treating dentofacial pain is less than ideal. It also confirms that individuals presenting to emergency departments with dental pain are a subset of the population of patients who are prescribed opioids as an analgesic. Coupling these results with the increased use of opioids in this country, dentists are in a key position to collaborate with emergency department physicians to help positively affect change. To further justify this approach, research agendas must carefully monitor prescribing patterns for dentofacial pain in the emergency department that are specific to opioid use, carefully excluding other non-narcotic analgesics. Should comparable outcomes of data related to the same topic in other non-dental settings exist, additional areas in medicine that may benefit from this partnership may also be identified. Moving forward, this interprofessional team approach may include a presence in medical school and residency program curricula so that alternative treatment options for addressing dental pain patients can be presented that consider the increased prescribing trends of opioids.
University Library System, University of Pittsburgh
2019-06-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
review
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/85
10.5195/d3000.2019.85
Dentistry 3000; Vol. 7 No. 1 (2019); 1-6
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/85/83
Copyright (c) 2019 Sara Barna, Jean O'Donnell, Marnie Oakley
oai:ojs.dentistry3000.pitt.edu:article/86
2020-04-15T00:49:27Z
dentistry3000:DoCS
Applicability of Moyer’s prediction tables to estimate the mesio-distal diameter of canines and premolars
de Sousa Galdino, Amanda
Braga Ramos, Tânia
Marques Filgueiras, Vitor
Wanderley Lacerda, Rosa Helena
mixed dentition
orthodontics
malocclusion
Objective: The aim of this study was to verify the applicability of Moyer’s prediction tables to estimate the mesio-distal diameter of canines and pre-molars in a population of North-Eastern Brazil. Materials and Methods: A sample of 100 plaster moulds of 12-18-year-old patients was selected from the dental archives of the Brazilian Association of Orthodontists Dental School Clinic in the city of João Pessoa, State of Paraiba. Analyses were performed with R software (version 3.1.3). The upper and lower canines and premolars on both sides of the arch were measured. Data Analysis: Linear regression analysis was used to assess the closeness of the estimated values in each percentile of Moyer’s prediction table. Analyses were performed at a significance level of 5%. Results: Gender dimorphism (P < 0.001) was found in both upper and lower arches. In the upper arch, the best regression models for data analysis of female, male and both were, respectively, p5 and p6”, p25 and p35, and p35 and 75, whereas in the lower arch were p15, p35 and p75, with “p75 being the best-fitted percentile following application of correction equations for both genders. Conclusions: The Moyer’s prediction table can be applied for estimating the mesio-distal diameters of canines and pre-molars, provided that a correction factor is used for the population under study.
University Library System, University of Pittsburgh
2019-06-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/86
10.5195/d3000.2019.86
Dentistry 3000; Vol. 7 No. 1 (2019); 27-32
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/86/82
Copyright (c) 2019 Rosa Helena wanderley lacerda
oai:ojs.dentistry3000.pitt.edu:article/88
2020-04-15T00:49:27Z
dentistry3000:MoOD
Absence of history of oral cleft in first-degree relatives of patients with prostate cancer
de Alvarenga Diniz Fonseca, Cláudia
Reis Barbosa Martelli, Daniella
Freitas Almeida, Ianná Luana
Hassen Sales, Galeno
Soares de Andrade, Rodrigo
Oliveira Dias, Verônica
Monteiro de Barros, Letízia
Martelli Júnior, Hercílio
Oral clefts
cancer
prostate
Objective: To evaluate the occurrence of nonsyndromic cleft lip and/or palate (NSCL/P) in families of patients with prostate cancer (PC).Study design: We conducted a case-control study involving a total of 748 individuals, 280 of which had PC, and 468 were free-cancer healthy individuals. The patients answered a questionnaire with basic demographic information and family history of NSCL/P in first-degree relatives. The information collected was stored in a database and analyzed by using the statistical program SPSS® 24.0 for Windows (Chicago, IL, USA). In order to determine the association with NSCL/P, chi-square and Fisher’s exact test and odds ratio (OR) with its 95% confidence interval (95% CI) for risk magnitude assessment. Values with p<0.05 were considered statistically significant.Results: Of total patients with PC, 2 had a positive history of NSCL/P. In the control group, 7 patients reported family history of NSCL/P (1df chi-square, p=0.34; Fisher´s exact test, p=0.49). The average age of the cases diagnosed with PC was 71.35±7.70 years, and control group was 64.42±9.67 years.Conclusion: Despite the limited population, the frequency of NSCL/P was not significantly increased in the first-degree relatives of patients with PC. Studies with larger samples and molecular analyses are needed to better understand the possible relationships in the etiology of cancer and NSCL/P.
University Library System, University of Pittsburgh
2019-07-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/88
10.5195/d3000.2019.88
Dentistry 3000; Vol. 7 No. 1 (2019); 37-42
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/88/85
Copyright (c) 2019 Cláudia de Alvarenga Diniz Fonseca, Daniella Reis Barbosa Martelli, Ianná Luana Freitas Almeida, Galeno Hassen Sales, Rodrigo Soares de Andrade, Verônica Oliveira Dias, Letízia Monteiro de Barros, Hercílio Martelli Júnior
oai:ojs.dentistry3000.pitt.edu:article/89
2020-04-15T00:49:27Z
dentistry3000:I%26A
Investigation of surface topography of different root-end filling materials: An in vitro study
Koruyucu, Mine
Özcan, Hazal
Bayram, Merve
Cankaya, Abdullah Burak
Keklikoglu, Nurullah
Seymen, Figen
Surface topography
root-end filling materials
retrograde filling
Aim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials.Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group dimidiated two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling.Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plague. A2,B2,C2 samples have more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2.Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution was more than normal saline solution for all groups as a hydroxiapatite crystals.
University Library System, University of Pittsburgh
2019-07-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
dental materials
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/89
10.5195/d3000.2019.89
Dentistry 3000; Vol. 7 No. 1 (2019); 7-12
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/89/84
Copyright (c) 2019 Mine Koruyucu, Hazal Özcan, Merve Bayram, Abdullah Burak Cankaya, Nurullah Keklikoglu, Figen Seymen
oai:ojs.dentistry3000.pitt.edu:article/90
2020-04-15T00:49:27Z
dentistry3000:I%26A
Aesthetic Perception and psychological impact of molar-incisor hypomineralisation among patients and parents
da Silva, Fernanda Mafei Felix
Vasconcelos Cruz, Christiane
Leal, Luise
de Castro Costa, Marcelo
children
parents
aesthetic
incisor
molar
Abstract The aim of this study was investigate`s patient and parent’s aesthetic perception and psychological impact of molar-incisor hypomineralisation (MIH) and to assess the correlation of MIH with caries experience. The sample comprised 56 children, 28 with MIH and 28 without MIH (comparison group), and their gardens (n=56). For the perception data we used the Child and Parent’s Questionnaire of Teeth Appearance. We used the European Academic of Paediatric Dentistry (EAPD) criteria to define MIH. Caries experience was assessed by Decayed, Missing and Filled Teeth (DMFT) index. Chi-square, Student’s t-test and correlation tests were used with significance level set at 5%. Most children were male (n=35; 62.5%) and patients with MIH perceived their affected teeth as stainned (p=0.01). MIH was considered by parents (6.96±1.7) to enhance psychosocial condition (social, physical, and psychological social) of their children (p<0.01). Patients with severe MIH showed the worst perception about the color of the teeth (p=0.07). There was no correlation between DMFT scores and presence of MIH (p=0.80). Patients and parents perceived MIH. Groups with severe MIH (children/ parents) showed the worse aesthetic perception.
University Library System, University of Pittsburgh
2019-07-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/90
10.5195/d3000.2019.90
Dentistry 3000; Vol. 7 No. 1 (2019); 13-20
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/90/86
Copyright (c) 2019 Fernanda Mafei Felix da Silva, Christiane Vasconcelos Cruz, Luise Leal, Marcelo de Castro Costa
oai:ojs.dentistry3000.pitt.edu:article/91
2020-04-15T00:49:27Z
dentistry3000:DoCS
Evaluation of Salivary and Oral Cell Collection Methods for Genomic DNA Extraction
Fernandez, Clarissa Christina Avelar
Ferreira, Fernanda Farage da Costa Felipe
Vasconcellos Cruz, Christiane
Costa, Marcelo de Castro
DNA
Saliva
Genetics
The use of saliva and oral cells as sources of biological material has gained attention, due to advantages such as facility, non-invasiveness, and great patient acceptance. The objective of the study was to compare four different types of saliva and oral buccal cell collecting methods for genomic DNA extraction: (1)Expectoration of saliva, (2)Expectoration of saliva with lingual stimulation, (3)Scraping with cytological brush, and (4)Scraping with cytological brush and expectoration of saliva. The sample was composed of students and employees from the Dental School of the Federal University of Rio de Janeiro (n = 20, 10 men and 10 women with mean age of 47.60 ± 15.70 and 20.50 ± 2.1, respectively). The collections were performed with an interval of at least one day between them and the participants were instructed to stay for less than 30 minutes without eating food and brushing teeth. Samples were stored at -20°C until DNA extraction was performed using a commercially available kit (Qiagen®). Differences in DNA yield between methods were test for statistical significance with an alpha of 0.05. No sexual dimorphism was observed in relation to the concentration of DNA (p=0.76), age (p=0.91), and ethnicities (p=0.72). There was no significant difference between the collection methods in relation to the quantity and purity of the extracted DNA (p≥0.05). All methods gave lower DNA yields than the ones obtained from blood or saliva collected through comercial kits and maybe carefully use forclinical diagnostic purposes or for research experiements requiring higher DNA concentrations.
University Library System, University of Pittsburgh
2019-07-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/91
10.5195/d3000.2019.91
Dentistry 3000; Vol. 7 No. 1 (2019); 33-36
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/91/87
Copyright (c) 2019 Clarissa Christina Avelar Fernandez, Fernanda Farage da Costa Felipe Ferreira, Christiane Vasconcellos Cruz Alves Pereira, Marcelo de Castro Costa
oai:ojs.dentistry3000.pitt.edu:article/94
2022-01-04T02:03:28Z
dentistry3000:A%26tE
Comparative evaluation of retentive strength of polyvinyl siloxane impression materials to custom tray using different tray adhesives: An In Vitro study
Devassy, Jinsa P
Pradeep, Nishna
Sreekumar, A V
George, Jimmy
James, Jittin
George, Jacob
Tray adhesive
Polyvinyl siloxane impression material
Retentive strength
The retentive strength of the impression material to the impression tray is an important factor that can affect the quality of the final impression. Separation of the impression from the tray or tearing of the impression while removing from the mouth could be one of the reasons for distortion. It is imperative to know the most effective tray adhesive which can be used with different brands of rubber-based impression material. So, this study was directed to compare the different tray adhesives that can be effectively used in our day-to-day clinical practice.
University Library System, University of Pittsburgh
2020-05-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/94
10.5195/d3000.2020.94
Dentistry 3000; Vol. 8 No. 1 (2020); 1-10
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/94/89
Copyright (c) 2020 Jinsa P Devassy, Nishna Pradeep, A V Sreekumar, Jimmy George, Jittin James, Jacob George
oai:ojs.dentistry3000.pitt.edu:article/96
2022-01-04T02:03:28Z
dentistry3000:DoCS
Dental anomalies in orthodontic patients with and without skeletal discrepancies
Christina Avelar Fernandez, Clarissa
Gentil Mattos, Mônica
Vasconcellos Cruz Alves Pereira, Christiane
Costa, Marcelo de Castro
malocclusion
dental abnormalities
dental development
Objective:To determine whether individuals withskeletal discrepancies of Class II or IIIdisplay a higher frequency of dental anomalies in comparison with individuals with Class I malocclusion. Design:A systematic search of the main electronic medical scientific literaturedatabases was conducted. Observational studies were selected if mentioning dental anomalies in the different skeletal malocclusion patterns.Results:A total of 4,768 studies were found and the duplicated studies (1,279) were removed, resulting in 3,489 papers to be analyzed. After screening by title, 138 were fit for screening by abstract. After that, a total of 13 papers were carefully read in full. Five studies included dental anomaly frequencies in orthodontic patients and included 7,679 participants. The frequency of dental anomalies ranged from 11.2% to 40.3%.It was observed that individuals with skeletal discrepancies of Class II and III had more dental anomalies when compared to individuals with Class I.Conclusion:Individuals with skeletal malocclusion patternshave more dental anomalies and there is an association between dental anomalies and skeletal Class II or Class III malocclusion patterns.
University Library System, University of Pittsburgh
2020-04-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/96
10.5195/d3000.2020.96
Dentistry 3000; Vol. 8 No. 1 (2020); 24-29
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/96/88
Copyright (c) 2020 Clarissa Christina Avelar Fernandez, Mônica Gentil Mattos, Christiane Vasconcellos Cruz Alves, Marcelo de Castro Costa
oai:ojs.dentistry3000.pitt.edu:article/99
2022-01-04T02:03:28Z
dentistry3000:A%26tE
Tobacco use cessation efforts among private dental practitioners of Delhi, India
Mehta, Abhishek
Kaur, Gurkiran
Popli, Rishabh
Khurana, Suchi
tobacco use cessation
dentists
questionnaire
nicotine replacement
India
Introduction: Tobacco use remains the single largest cause of premature death around the world. Motivating tobacco users through behavioral counseling is the mainstay for achieving tobacco abstinence. Research evidence suggests a larger role of dental professionals in motivating tobacco users to quit. The efforts of private dental practitioners in providing Tobacco Cessation Interventions (TCI) in their clinics need to be measured and acknowledged.Methods: A cross-sectional survey based on self-administered questionnaire with private dental practitioners was carried out in the state of Delhi, India. The main objectives of the survey were to assess the current practices and the barriers experienced in providing TCI by the participating dentists. Statistical analysis was conducted to find out the association between independent variables and the responses to questions on tobacco cessation.Results: The mean age and years of practice of 297 participating dentists was 37.2 (SD 9.8) and 11.9 (SD 9.5) years respectively. Most of them were graduate (N=204, 68.7%). Only 17.2% (N=51) of the participating dentists had undergone any training in providing TCI. Most of the dentists felt that the lack of proper training (N=146, 49.2%) and resistance by patients (N=185, 62.5%) are the main barriers in providing TCI. Younger dentists (<35 years) performed significantly better than older dentists in many parameters pertaining to attitude and practices of TCI in their clinics.Conclusion: This is a pioneer survey conducted on the issue of TCI provided by private dental practitioners of Delhi. The study results show that around 2/3rds of the participating private dental practitioners were providing TCI to their patients but there are certain barriers for its proper implementation. Therefore, there is an urgent need to remove these self-reported barriers and build self-confidence among the dentists for instituting effective TCI in their clinics.
University Library System, University of Pittsburgh
2020-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
cross-sectional
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/99
10.5195/d3000.2020.99
Dentistry 3000; Vol. 8 No. 1 (2020); 11-23
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/99/91
Copyright (c) 2020 Abhishek Mehta, Gurkiran Kaur, Rishabh Popli, Suchi Khurana
oai:ojs.dentistry3000.pitt.edu:article/101
2022-01-04T02:19:24Z
dentistry3000:DoCS
Multifactorial Contributions to Central Incisor Tooth Morphology and the Presence of Black Triangles: A Case Study
Kohane, Steven E.
Means, Katelyn C.
black triangles
multifactorial inheritance
cosmetic dentistry
esthetics
gingival embrasures
interdental papilla
contact point
tooth morphology
odontometry
Abstract: Dentists are often faced with patient concerns of smile esthetics in addition to oral health and should be equipped to help patients manage both. Black triangle disease, or a lack of interdental papilla, is esthetically displeasing particularly in the central incisor region and has a hoard of etiologies ranging from gum disease to inherited tooth morphologic features. Addressing patients’ esthetic concerns must first be met with understanding the causation of the black triangles before treatment can be rendered. Genetic contributions are multifactorial, and this case report provides evidence for a case of a central incisor black triangles without other affected family members.Background: Black triangles are a prominent feature for those experiencing a lack of interdental papilla between central incisors and may dramatically affect the presentation of a person’s smile. The aim of this case report is to convey the importance of managing patients’ esthetic chief complaints while addressing the etiology of the esthetic concern.Case Description: A 24-year-old man presented to his dental colleague with concern of his black triangle spaces. The patient does not have gingival recession in the area of interest, and his biological mother and brother do not have black triangle spaces. Pathology is not an issue, but instead inadequate length of the central incisor mesial contact area prevents the interdental papilla from reaching the contact. Esthetics are a concern, but the patient is not interested in irreversible treatment for his healthy tissue.Practical Implications: Dentists should be aware of the multifactorial contributions to patients’ esthetic concerns. Dentists should also be aware that meeting a patient’s expectations may require a more complex treatment plan than the patient expected.
University Library System, University of Pittsburgh
2021-03-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case Report
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/101
10.5195/d3000.2021.101
Dentistry 3000; Vol. 9 No. 1 (2021); 161-166
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/101/95
Copyright (c) 2021 Steven E. Kohane, Katelyn C. Means
oai:ojs.dentistry3000.pitt.edu:article/117
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Effects of a novel dye containing toothpaste versus regular fluoridated toothpaste for supragingival biofilm reduction in patients with chronic gingivitis: A Randomized Controlled Trial
Rath, Avita
Jun Ning, Chong
HR, Priyadarshini
Teo, Brandon Nicholas
Zhen, Naik Hsian
Yin, Ong Wey
dental biofilm
supragingival
gingivitis
oral hygiene
dentifrice
AIM:To compare the effects of a plaque disclosing toothpaste with a regular fluoridated toothpaste for supragingival biofilm reduction in patients with chronic gingivitis.MATERIALS AND METHODS:This study was a randomized controlled trial with a parallel design. Forty patients, both males and females aged 18-25 years with chronic generalized gingivitis (N=40) were randomly allocated to two groups of twenty each (n=20), using lottery method. Participants in the experimental group brushed with a plaque disclosing toothpaste, while participants in the control group brushed with regular fluoridated toothpaste. Clinically, biofilm was measured using Silness & Loe Plaque Index, both at baseline and at follow up visits by two assessors who were blinded to group allocation. Adjunctly, intraoral photographs were taken for Digital Plaque Image Analysis of the remaining dental biofilm in the upper and lower anterior labial tooth surfaces, and calculated based on the area of stained pixels. Data was analyzed using SPSS version 22. Mean plaque scores between the groups were compared using independent sample t-test and p<0.05 was considered significant.RESULTS:Data of all the 40 participants were analyzed as they were no drop outs. No significant differences were found in the mean plaque scores between the two groups (p=0.059) at the end of the study. Similarly, Digital Plaque Image Analysis showed no significant difference in the mean plaque scores between the groups (p= 0.93).CONCLUSION:This study showed that there are no additional benefits of using a plaque disclosing toothpaste, in enhancing the removal of the supragingival dental biofilm during routine oral hygiene maintenance.
University Library System, University of Pittsburgh
2021-06-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Randomized controlled trial
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/117
10.5195/d3000.2021.117
Dentistry 3000; Vol. 9 No. 1 (2021); 46-55
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/117/98
Copyright (c) 2021 Avita Rath, Chong Jun Ning, Priyadarshini HR, Brandon Nicholas Teo, Naik Hsian Zhen, Ong Wey Yin
oai:ojs.dentistry3000.pitt.edu:article/118
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Influence of occlusal anatomy on acrylic resin CAD/CAM crowns fracture load and stress distribution.
Borges, Alexandre Luiz Souto
de Lima, Aline Lins
Campaner, Larissa Mendes
Bottino, Marco Antonio
Dal Piva, Amanda Maria de Oliveira
Tribst, João Paulo Mendes
Finite Element Analysis
Computer-Aided Design
Crowns
Acrylic Resins
Weight-Bearing
Objectives: This study compared the influence of occlusal anatomy on acrylic CAD/CAM crowns fracture load and stress distribution. The null hypothesis was that there would be no difference between the provisional crowns fracture load and stress according to different occlusal anatomy.Methods: A full-crown preparation was simulated using dentin analogue (G10, Protec, São Paulo, Brazil) totaling 20 identical preparations. Next, twenty acrylic crowns were milled using different occlusal design parameter (Young or Adult) available in the software database. The crowns were cemented (Temp-bond, NE Kerr Dental, Brea, CA, USA) and fractured using a compressive load (0.5 mm/min of cross-head speed). Data were analyzed by using one-way ANOVA and Tukey tests (p< 0.05). A similar geometry was modeled and exported to the analysis software to perform a static structural analysis. The maximum principal stress was calculated using the finite element method with 300 N chewing load simulation.Results: The occlusal anatomy significantly influenced the load-to-fracture (p<0.05). Young design showed lower fracture load (1139±132 N) than Adult design (2007±345 N). The tensile stress distribution showed a similar pattern for both groups however the highest stress peak was calculated for Young design (76 MPa) in the occlusal surface.Conclusion: The anatomy design with higher cusp angulation and occlusal sulcus more evident can increase the stress concentration and reduce the fracture load for acrylic resin CAD/CAM crowns.
University Library System, University of Pittsburgh
2021-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
in vitro; in silico
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/118
10.5195/d3000.2021.118
Dentistry 3000; Vol. 9 No. 1 (2021); 36-45
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/118/97
Copyright (c) 2021 Alexandre Luiz Souto Borges, Aline Lins de Lima, Larissa Mendes Campaner, Marco Antonio Bottino, Amanda Maria de Oliveira Dal Piva, João Paulo Mendes Tribst
oai:ojs.dentistry3000.pitt.edu:article/119
2022-01-04T02:19:24Z
dentistry3000:A%26tE
The correlation of perceived stress and professional concerns during COVID-19 pandemic among Iranian dentists: the mediating role of cognitive flexibility
Afshari, Afrooz
Hashemikamangar, Seyedehsareh
Hashemikamangar, Sedighe Sadat
COVID-19
dentists
professional concerns
perceived stress
psychological flexibility
Objective: To evaluate the association of perceived stress and dental work-related concerns regarding to COVID-19 by mediating role of cognitive flexibility among dentists during pandemic outbreak of COVID-19 in Iran.Methods: This study was a cross sectional survey conducted by means of a confidential on-line survey on 174 Iranian dentists. COVID-19 related questions were divided to three parts: dentists’ perspective about their career future, virus contagion and concerns of getting infected COVID-19 from patients, and paucity of information and facilities to control the spread of the virus. Perceived stress and psychological flexibility were assessed by PSS (Cohen et al., 1983) and CFI (Dennis et al., 2010). To analyze data path analysis method was used.Results: Perceived stress significantly predicted psychological flexibility (both alternatives and control subscales) and COVID-19 factors related to dental work In addition, control subscale significantly predicted dentists’ concerns toward their career future and fear of COVID-19 contagion. Hence, control subscale of cognitive flexibility mediated the relationship of perceived stress and professional concerns of dentists during COVID-19 pandemic.Conclusion: Given these results, elevated stress during an infectious disease outbreak can effect (have impact on) perceived stress and professional concerns among dentists. Different aspects of dentists’ lives can be affected by those concerns and stress. Interventions to enhance the cognitive flexibility should be applied to reduce the psychological burden of the crisis for dentists.
University Library System, University of Pittsburgh
2021-01-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
survey
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/119
10.5195/d3000.2021.119
Dentistry 3000; Vol. 9 No. 1 (2021); 1-10
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/119/92
Copyright (c) 2021 Afrooz Afshari, Seyedehsareh Hashemikamangar, Sedighe Sadadat Hashemikamangar
oai:ojs.dentistry3000.pitt.edu:article/120
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis
Godil, Aamir Zahid
Bhagat, Diksha
Das, Parijat
Kazi, Ashi Ilyas
Dugal, Ramandeep
Satpute, Surekha
Antifungal agents
biofilms
scanning electron microscope
soft liners
hardness
surface roughness
minimal inhibitory concentration
denture stomatitis
Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.
University Library System, University of Pittsburgh
2021-02-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Experimental - In-vitro
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/120
10.5195/d3000.2021.120
Dentistry 3000; Vol. 9 No. 1 (2021); 11-22
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/120/93
Copyright (c) 2021 Aamir Zahid Godil, Diksha Bhagat, Parijat Das, Ashi Ilyas Kazi, Ramandeep Dugal, Surekha Satpute
oai:ojs.dentistry3000.pitt.edu:article/121
2022-01-04T02:19:24Z
dentistry3000:I%26A
The relationship between dental fear and cooperation of children during dental treatments with their parents’ general health
Daneshvar, Seyedeh Hediyeh
Azizi, Saeedeh
Fear
Behavior
Health
Dentistry.
Objective: Dental fear is a common phenomenon in children and parents can have a key role in the development of child's dental anxiety. There is moderate evidence to support the relationship between the parental general health and children’s dental fear. The aim of this study was to investigate the relationship between dental fear and cooperation of children during dental treatment with the general health of parents.Methods: This cross-sectional descriptive study was done on 130 children aged 6 to 12 at the Department of Pediatric Dentistry, Guilan University of Medical Sciences from May to September 2020.The personality traits and general health of parents were assessed by using the General Health Questionnaire (GHQ-28). The Frankl's behavior rating scale and Children's Fear Survey Schedule Dental Subscale (CFSS-DS) were used to respectively assess the degree of cooperation during dental practices and children’s dental fear. Statistical approaches included T-test, Chi-square and Pearson Linear correlation. A significant level of differences was taken as P<0.05.Results: 69.3 % of children behaved positively and definitely positively during dental treatment. 56 boys and 74 girls with mean age of 9.13 ±2.02 years participated in this study. There was a significant correlation between the children's age with dental fear and cooperation level (P<0.001). There was no significant difference in dental fear score (P=0.63) and cooperation level (P=0.99) between boys and girls. There was not statistically significant relationship between the general health of the parents and the level of children's cooperation and dental fear during dental treatment (P>0.001).Conclusion: There was not relationship between parent's general health with children's dental fear and cooperation.
University Library System, University of Pittsburgh
2021-06-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/121
10.5195/d3000.2021.121
Dentistry 3000; Vol. 9 No. 1 (2021); 118-126
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/121/99
Copyright (c) 2021 Seyedeh Hediyeh Daneshvar, Saeedeh Azizi
oai:ojs.dentistry3000.pitt.edu:article/124
2022-01-04T02:19:24Z
dentistry3000:I%26A
A Clinical and Radiographic Assessment of Sodium Hypochlorite Versus Formocresol Pulpotomy in Primary Molar Teeth: 12-month Follow-up.
Bahrololoomi, Zahra
Zarebidoki, Fatemeh
Shakib, Atefeh
Pulpotomy
Primary Teeth
Formocresol
Sodium Hypochlorite
Objective: The aim of this study was to compare the clinical and radiographic success rates of pulpotomy in primary molars using formocresol versus sodium hypochlorite.Methods: Twenty-three children aged 4-9 years with at least two primary molars requiring pulpotomy were randomly allocated into two groups. All teeth received stainless steel crown after conventional pulpotomy procedure with either NaOCl or formocresol. Clinical and radiographic signs/symptoms were recorded at six and 12 months. Outcomes were statistically analyzed using Fisher’s exact test and Chi-squaretest.Results: Clinical success rates at 6 and 12-month follow-up in both groups was 100%. At 6-month follow-up, radiographic success rate for NaOCl and formocresol groups was 100%. At 12-month recalls, in NaOCl group, 20 teeth (87%) and in formocresol group, 21 teeth (91.3%) had radiographic success. No significant difference was found in the radiographic success rates at 12 months (P=1.00). Internal root resorption was the most common radiographic pathologic finding in both groups.Conclusion:Clinical and radiographic success rates in NaOCl group was comparable with formocresol group, so NaOCL can be suggested as an alternative for primary teeth pulpotomies. However further clinical studies with long-term follow-ups are needed.
University Library System, University of Pittsburgh
2021-07-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/124
10.5195/d3000.2021.124
Dentistry 3000; Vol. 9 No. 1 (2021); 127-134
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/124/103
Copyright (c) 2021 Zahra Bahrololoomi, Fatemeh Zarebidoki, Atefeh Shakib
oai:ojs.dentistry3000.pitt.edu:article/131
2022-01-04T02:19:24Z
dentistry3000:A%26tE
In-Vitro study to Assess Effect of a Glide Path on Canal Transportation and Centering Ratio in Double Curved canals.
Alhalabi, Raghad
Rekab, Mohammad Salem
Alhroob, Khaldaon hossein
Al-assaf, Maher
endodontic treatment
canal centric
R-Pilot system
deviation amount.
Objective: In endodontic treatment, creating a glide path became one of the recommended essential principles to obtain a safe preparation as possible; so the aim of the research is to evaluate the efficiency of the R-Pilot system in glide path preparation within the double curved canals.Study design: A laboratory study to assess the efficiency R-Pilot mechanical patency system on the shape marinating of the canal. Those criteria were used to evaluate the change in the original shape of the canal, Canal Transportation and Centering Ratio. The data were analyzed using SPSS V.13 program and the correlation between the variables were defined using descriptive statistics and inductive statistical tests (chi-square, T Student for independent samples, ANOVA).Results: no significant difference between the two glide path creation systems was found in terms of the deviation amount from the canal centric. whereas, between the R-Pilot system and the manual preparation, significant difference was found in the preserving rate of the canal centric at both the coronal area and the entire canal.Conclusion: glide path creation in the previously mentioned methods with confirmation on the importance of the mechanical patency in terms of speed, Reducing the effort and burden imposed on the practitioner compared to the use of manual files. XP Endo shaper had lower deviation values and more ability to save the shape and centric of the canal.
University Library System, University of Pittsburgh
2021-07-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/131
10.5195/d3000.2021.131
Dentistry 3000; Vol. 9 No. 1 (2021); 56-67
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/131/102
Copyright (c) 2021 Raghad Alhalabi, Mohammad Salem Rekab, Khaldaon hossein Alhroob, Maher Al-assaf
oai:ojs.dentistry3000.pitt.edu:article/134
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Is YoutubeTM an accurate source of patient-information for awareness about periodontal diseases?
Güler, Berceste
Özaltun, Büşra
YouTube
oral hygiene
periodontitis
awareness
information
Objectives: Oral hygiene education and patient awareness are crucial for prevention and the sustainability of its treatment because of high prevalence of periodontal diseases. This study aimed to evaluate YouTube videos related to periodontal diseases and assess them comprehensively, reliability, and quality for non-professional internet users.Methods: YouTube search was performed using the three keywords: ‘periodontal disease’, ‘gingival disease,’ and ‘gum disease’. Video lengths, duration, numbers of total views, likes, dislikes, comments values were recorded. The interaction index, viewing rates and video power index (VPI) were calculated. Comprehensiveness tailor-made index was assessed for content, Global Quality Scale (GQS), and DISCERN scales were used for reliability and quality of videos.Results: A total of 210 videos were evaluated, and 79 videos were included in the study. While 69 of the videos are useful videos, 9 of them have misleading video content. VPI values were found 2.88±0.67 for useful videos and 1.78±0.66 for misleading videos. The mean GQS value of the videos has seen as 2.76±0.7. According to the DISCERN score, 41.8% of the videos show poor quality. The number of videos with comprehensiveness value (2) score is 44 and the number of videos (1) score is 35.Conclusions: YouTube videos may be used as an education source about periodontal disease for non-professional users; however, videos need to be improved in terms of content and quality. These and similar publications may be supported for the optimization of videos to be shared on YouTube with periodontal disease and oral hygiene education.
University Library System, University of Pittsburgh
2021-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
cross-sectional
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/134
10.5195/d3000.2021.134
Dentistry 3000; Vol. 9 No. 1 (2021); 23-35
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/134/96
Copyright (c) 2021 Berceste Güler, Büşra Özaltun
oai:ojs.dentistry3000.pitt.edu:article/138
2022-01-04T02:19:24Z
dentistry3000:DoCS
Long term radiographic evaluation of a potentially impacted and dilacerated tooth into complete transposition
shafiee, Hassan Ali
Nahvi, Golnaz
Aghdashi, Farzad
Karami, Shahryar
Tooth transposition
ectopic eruption
maxillary canine
orthodontic treatment
Background: Anomalies such as ectopic tooth eruption and transposition are challenging situations that may complicate the orthodontic treatment. Some of these anomalies can only be diagnosed and managed by accurate long-term radiographic examination. Delayed diagnosis could impede simple preventive orthodontic measurements and cause more costly and consuming future complicated orthodontic treatment.Case Description: We described in detail the long term follow-up of a young female patient referring with a unilateral ectopic eruption. The patient was under close observation and regular orthodontic treatment to guide a unilateral ectopic eruption into a complete transposition of an upper left canine and first premolar. The long term follow-up revealed stables results.Practical Implications: Early diagnosis and preventive measurements in patients suffering from anomalies such as ectopic eruption is crucial in order to avoid further complications such as tooth impaction and dilaceration formation and adopting the best choice of treatment.
University Library System, University of Pittsburgh
2021-07-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/138
10.5195/d3000.2021.138
Dentistry 3000; Vol. 9 No. 1 (2021); 167-177
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/138/108
Copyright (c) 2021 Hassan Ali shafiee, Golnaz Nahvi, Farzad Aghdashi, Shahryar Karami
oai:ojs.dentistry3000.pitt.edu:article/139
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Evaluation of the Most Common Dental Procedural Errors Leading to Lawsuits and the Relevant Reasons in Three Dental Clinics in Tehran
Yazdanian, Mohsen
Rafiei, Esmail
Rahmani, Aghil
Tahmasebi, Elahe
Hajiesfandiari, Abbas
One of the most important occupational stresses that dentists encounter in their profession is patients’ lawsuits. The present study aimed to evaluate common procedural errors that lead to lawsuits in dentistry. In the present cross-sectional study, all the patient files of three large dental clinics in Tehran were evaluated from 2014 to 2019, in which a dentist’s negligence had been confirmed by the specialty dental committee. Files with incomplete clinical data, without the dentist’s name, and the type of the lawsuit were excludeda and Finally, 107 lawsuit files were confirmed and entered the study. After extraction of the data from the existing files and completing the relevant checklist, data were analyzed with SPSS 23. Most lawsuits were of the therapeutic type (88 cases, 82%), with 34 cases (31.9%) , 26 cases (24.2%) and 19 cases (17.6%) related to prosthodontics, endodontics, and surgery, respectively. Most plaintiffs were 30–49 years of age (56 cases ,52%), and most were women (70 cases, 65%). Over half of the lawsuits had been filed against dentists with a job experience of <10 years and against general dental practitioners (81 cases, 76%). One of the most important ways to prevent errors leading to lawsuits is to increase dentists’ knowledge about previous lawsuits. Knowledge about the most common lawsuits and the provision of organizational guidelines will help manage and decrease such lawsuits.
University Library System, University of Pittsburgh
2021-08-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/139
10.5195/d3000.2021.139
Dentistry 3000; Vol. 9 No. 1 (2021); 68-76
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/139/109
Copyright (c) 2021 Mohsen Yazdanian, Aghil Rahmani, Elahe Tahmasebi, Abbas Hajiesfandiari
oai:ojs.dentistry3000.pitt.edu:article/141
2022-01-04T02:19:24Z
dentistry3000:I%26A
Oral health status, attitudes and gender variations among governmental school children at Al-Mafraq governate – Jordan
Smadi, Leena
Kharma, Lara
Abu Abed, Asma’a
Bny Mfarej, Eman
Abdalmohdi, Asma
Dental health
oral health
adolescents
dental caries
Objectives: Calculate the prevalence and severity of dental caries and periodontal health among school students aged 12-18 years old and their attitudes toward dental health and oral hygiene at Al-Mafraq governate- Jordan.Materials and methods: Demographic and oral behavioral information were collected; students aged 12-18 years old from six public schools distributed over three districts in Al-Mafraq governate over a six-month period had a full oral and dental examination. DMFT index (Decay, Missing, Filling, Teeth) was used to measure caries experience. Plaque index (PI) and gingival index (GI) of Löe and Silness were used to evaluate the oral health status. T - test was used for statistical evaluation. A P-value < 0.05 was considered statistically significant.Results: 1165 students were examined, 51% never had been to a dentist or had any previous dental treatment, 39.6% of the students examined never brushed their teeth and 61.85 % had dental caries. The (SiC) was 4.72 with a decay representing 93.53%. Females brush their teeth more frequent than males with a statistically significant difference; p< 0.001. Females showed lower DMFT index (1.81) and compared to males (2.25) with a statistically significant difference. Females showed lower SiC (4.164) compared to males (5.186) with a statistically significant difference. There was no statistically significant difference between males and females in PI p= 0.590, and GI; p= 0.852.Conclusions: The prevalence of dental caries among students in Al-Mafraq governate and the significant caries index were substantially higher than the target goals of WHO/ FDA of year 2015 with more than 90 % of unmet treatment needs. Girls showed better oral hygiene habits compared to boys and exhibited less dental caries experience. However, periodontal status did not differ significantly between both genders.
University Library System, University of Pittsburgh
2021-09-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
cross suctional public health survey
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/141
10.5195/d3000.2021.141
Dentistry 3000; Vol. 9 No. 1 (2021); 152-160
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/141/112
Copyright (c) 2021 leena smadi, Lara Kharma, Asma’a Abu Abed, Eman Bny Mfarej, Asma Abdalmohdi
oai:ojs.dentistry3000.pitt.edu:article/143
2022-01-04T02:19:24Z
dentistry3000:I%26A
Assessment of dehiscence and fenestration in children and adolescents using cone beam computed tomography
Akbulut, Aslıhan
Akgöl, Beyza Ballı
Orhan, Kaan
Bayram, Merve
alveolar bone defect
cone-beam computed tomography
dehiscence
fenestration
primary tooth
Objectives: To define the prevalence of dehiscence and fenestration and classify them in terms of the localization of fenestrations in a random sampled group of children and adolescent patients using cone-beam computed tomography (CBCT).Methods: CBCT performed at the Department of Oral and Maxillofacial Radiology of patients referred by the paediatric dentistry clinic were included in this retrospective study. Image evaluations were performed by dentomaxillofacial radiologist (AA, asst. prof.), and these images were examined in three dimensions of the axial, coronal, and sagittal planes. Intraexaminer agreement for the evaluations were found acceptable. The presence/absence of dehiscence and/or fenestration, fenestration’s classification type, and localization of defects were recorded. Moreover, the presence/absence of periapical lesion in related root with dehiscence and fenestration was noted. For statistical analysis, The Chi-Square test, Fisher Freeman Halton Test, and Yates' Continuity of Correction were used.Results: 3061 roots in 1801 teeth of 120 cases were analyzed. The mean age was 9.97±2.22 years. Dehiscence was detected in 261(8.5%) roots of 161(8.9%) teeth, and fenestration was detected 63(2%) roots of 36(2%) teeth. The most common fenestration type was Type I, followed by Type II and IV. Dehiscence was observed more frequently in primary teeth than permanent teeth, and the difference was statistically significant (p:0.000). Dehiscence and fenestration incidence in maxillary teeth was significantly higher than in the mandibular teeth (pdehiscence:0.000, pfenestration:0.004). Apical lesions were observed more in primary teeth than permanent teeth for both defects.Conclusions: This study concludes that alveolar dehiscence and fenestrations are more common in primary teeth than permanent teeth. Moreover, these defects were detected more for the teeth in the maxilla. Concerning endodontic and orthodontic therapies in maxilla, use of CBCT is useful in determining the region's anatomical structure accurately in suspected cases of child and adolescent patients.
University Library System, University of Pittsburgh
2021-07-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/143
10.5195/d3000.2021.143
Dentistry 3000; Vol. 9 No. 1 (2021); 135-151
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/143/106
Copyright (c) 2021 Aslıhan Akbulut, Beyza Ballı Akgöl, Kaan Orhan, Merve Bayram
oai:ojs.dentistry3000.pitt.edu:article/146
2022-03-21T12:49:52Z
dentistry3000:MoOD
Mode of cell death of co-cultured dental mesenchymal stem cells following exposure to endodontic irrigating solutions
Saberi, Eshagh Ali
Mollashahi, Narges Farhad
Ahmadi, Mahboobe
Pirhaji, Arezoo
Co-culture
Apoptosis
Necrosis
Irrigants
Cells
Cultured.
Aims: This in vitro study aimed to determine the mode of cell death of co-cultured dental mesenchymal stem cells following exposure to different irrigating solutions.Methods and Material: Mesenchymal stem cells of the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs) were isolated from the periapical region of a third molar of an 18-year-old patient and cultured. Adequate number of cells in each well, 6-well plate inserts were placed over the wells. The cells were then randomly exposed to 17% ethylenediaminetetraacetic acid (EDTA), 1.5% sodium hypochlorite (NaOCL), 2% chlorhexidine (CHX) and saline. After 1, 5, and 10 minutes cells were trypsinized and underwent flow cytometry. Apoptotic and necrotic cells were quantified. Data were analyzed using the One-way ANOVA and Tukey’s post-hoc test. Results: With increasing exposure time, cell viability is decrease and the mode of cells death was necrosis in CHX and NaOCL groups and necrosis plus late apoptosis in 1 and 10 minutes in EDTA group.Conclusion: Maximum cell death occurred following exposure to EDTA while minimum cell death occurred following exposure to CHX. Necrosis was the dominant mode of cell death in all groups.
University Library System, University of Pittsburgh
2022-03-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/146
10.5195/d3000.2022.146
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/146/133
Copyright (c) 2022 eshagh ali saberi, narges farhad mollashahi, Mahboobe Ahmadi, arezoo pirhaji
oai:ojs.dentistry3000.pitt.edu:article/150
2022-01-04T02:19:24Z
dentistry3000:ER
Erratum to: Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis
Dugal, Ramandeep
Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance. Keywords: Antifungal Agents; Biofilms; Candida; Scanning Electron Microscope; Soft Liners; Hardness; Surface Roughness; Minimal Inhibitory Concentration; Denture Stomatitis.
University Library System, University of Pittsburgh
2021-02-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/150
10.5195/d3000.2021.150
Dentistry 3000; Vol. 9 No. 1 (2021)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/150/94
Copyright (c) 2021 Ramandeep Dugal
oai:ojs.dentistry3000.pitt.edu:article/155
2022-07-13T18:35:15Z
dentistry3000:I%26A
Prevalence of Malocclusion in Brazilian Quilombola Adolescents
de Brito, Gabrielli Oliveira
Martins, Sarah dos Santos
Paes de Araújo, Ítallo Lins
Carvalho Laureano, Isla Camilla
Cavalcanti, Alessandro Leite
Adolescent
Epidemiology
Ethnic Groups
Malocclusion
Objective: To evaluate the prevalence of malocclusion in Quilombola adolescents living in rural area in Northeastern Brazil.Material and Methods: This is a cross-sectional study in which 36 Quilombola adolescents aged 12-19 years were evaluated. Data collection was performed at home between January and April 2020 by a trained researcher using the Dental Aesthetic Index (DAI). Data were analyzed using the IBM SPSS software and presented using descriptive statistics.Results: More than two thirds of adolescents had already visited the dentist at least once in life (72.2%), with the last dental visit having occurred less than a year ago (57.7%), with predominance of public dental service (84.6%). There was higher frequency of lower anterior misalignment (86.1%) and dental crowding (83.4%) in two segments (55.6%). Almost all adolescents presented dental malocclusion (97.2%), with very severe or disabling severity (44.4%).Conclusion: The prevalence of very severe malocclusion was high in Quilombola adolescents, expressing the need for treatment of this population.
University Library System, University of Pittsburgh
2022-07-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/155
10.5195/d3000.2022.155
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/155/149
Copyright (c) 2022 Gabrielli Oliveira de Brito, Sarah dos Santos Martins, Ítallo Lins Paes de Araújo, Isla Camilla Carvalho Laureano, Alessandro Leite Cavalcanti
oai:ojs.dentistry3000.pitt.edu:article/156
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Stress analysis of over-denture abutments treated with two obturation techniques
Elsherbini, Ahmed N.
Nagy, Mohamed M
Elsherbini, Nancy N
Over Denture
Abutments
root canal obturation
endodontic obturation technique
stress analysis
Aim: Evaluation of stresses induced on over-denture abutments endodonticaly treated with two different obturation techniques.Materials and methods: eight mandibular permanent canines were inserted in four pesudo-realistic models. In each model two canines were placed in it. One canine was treated with lateral obturation technique and the other canine with vertical obturation technique. Four over-denture prostheses were fabricated on the four models. Then strain gauges were attached on the coronal one third of the root buccally and lingually for all eight canines. A metal rod was placed occlusally on the premolar –molar area, and a 50 N load was applied on the middle of the rod using the universal testing machine. Readings were collected. Results: from the collected data the lateral obturation showed 85.25±104.901 µε and the vertical obturation showed 4.875±3.555 µε with significance difference of P<0.05 between the groups.Conclusion: it can be concluded that the abutments treated with vertical obturation techniques reduced the stresses induced to the supporting structures when compared with the lateral condensation technique.
University Library System, University of Pittsburgh
2021-08-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/156
10.5195/d3000.2021.156
Dentistry 3000; Vol. 9 No. 1 (2021); 77-84
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/156/110
Copyright (c) 2021 Ahmed N. Elsherbini, Mohamed M Nagy, Nancy N Elsherbini
oai:ojs.dentistry3000.pitt.edu:article/157
2022-03-21T12:49:52Z
dentistry3000:DoCS
Comparative effects of Salvizan gel (containing Salvia officinalis extract) and triamcinolone acetonide gel on the duration and symptoms of oral lichenoid reactions
Ghalayani, Parichehr
Pakravan, Fahimeh
Abbasi, Fatemeh
Lichenoid reaction
Gel
Scarlet sage
Triamcinolone
Objectives: Lichenoid reactions are disorders with similar clinical presentations. The current study was conducted to compare the improvement indices in the patients’ symptoms by Salvizan and triamcinolone gels. Therefore, regardless of the cause, eliminating the pain and symptoms is one of the objectives of the treatment.Methods: This study was designed as a randomized double-blind clinical trial. Diagnosis of patients was performed by clinical examinations and confirmed by histological evaluation. One group of patients was administered Salvizan and another group was administered triamcinolone. After washing their mouth, the patients administered the gel (1 cm) on the inflammatory mucosa three times a day for two weeks. The amelioration rate of the lesions in each visit was measured from according to Visual Analog Scale (VAS) on a scale of 0-10.Results: The results of repeated measures ANCOVA showed a significant difference between groups in terms of the pain intensity before treatment (p<0.001). Moreover, there was a significant difference between different times with regard to pain intensity (p=0.004). To evaluate the clinical indices in the study groups, Mann-Whitney test was used and the results showed no significant difference for the extent of lesions before treatment (p=0.874), after one week (p=0.503) and after two weeks (p=0.669).Conclusions: Salvizan could better reduce pain intensity than triamcinolone, but clinically no significant difference was reported between the drugs in terms of the extent of lesion.
University Library System, University of Pittsburgh
2022-03-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/157
10.5195/d3000.2022.157
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/157/124
Copyright (c) 2022 Parichehr Ghalayani, Fahimeh Pakravan, Fatemeh Abbasi
oai:ojs.dentistry3000.pitt.edu:article/158
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Comparison of force decay pattern in orthodontic elastomeric chains and NiTi closed coil springs, affected by five different mouthwashes: An in vitro Study
Mirhashemi, Amir Hossein
Habibi Khameneh, Niloofar
Shahpoorzadeh, Keyvan
Saffar Shahroudi, Atefe
Force reduction
orthodontics
mouthwash
elastomeric chain
closed coil springs
Background: The aim of this study was to compare the force decay pattern of elastomeric chains and NiTi coil springs which were exposed to five different commercially available mouthwashes.Methods: In this in vitro study, 60 pieces of elastomeric chain (EC) and 60 NiTi closed coil springs (CS) were divided into 6 groups. The specimens were exposed to one of these mouthwashes twice a day for 60 seconds: Listerine, chlorhexidine, Orthokin, Persica, fluoride and artificial saliva as the control group. The elastomeric chains and NiTi springs were stretched so that they exert the initial force of 250 gr. Their force was measured on the 1, 7, 14 and 28 following days by means of a digital gauge.Results: Elastomeric chains and coil springs had force decrease over time, but EC' force reduction was greater with the highest reduction rate in the first week. However, in the CS group, the force decrease wasn't statistically significant in the first two weeks. After 28 days in the control groups, 49.8% of the initial force was remained in ECs while the value was 93.3% for CSs. In comparison between mouthwashes, in EC groups, in all mouthwashes except Persica, the remained force was statistically less than control group.Conclusion: Force degradation of elastomeric chains could be exacerbated by use of mouthwashes. About coil springs, force decay was also observed. However, it was not statistically significant. Force reduction was detectable after four weeks of coil springs usage, but in EC groups, the greatest reduction was after the first week.
University Library System, University of Pittsburgh
2021-10-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/158
10.5195/d3000.2021.158
Dentistry 3000; Vol. 9 No. 1 (2021); 95-106
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/158/115
Copyright (c) 2021 Amir Hossein Mirhashemi, Niloofar Habibi Khameneh, Keyvan Shahpoorzadeh, Atefe Saffar Shahroudi
oai:ojs.dentistry3000.pitt.edu:article/159
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Do different time intervals in placement of restorative materials over calcium silicate cements, affect interface microhardness of different restorative materials?
Bolhari, Behnam
Meraji, Naghmeh
Khazaee, Pegah
Ghabraei, Sholeh
Valizadeh, Sara
Calcium silicate cement
restorative materials
microhardness
time intervals
Objectives: The type of materials and application time of final restorations on calcium silicate cements (CSCs) are important factors which influence the interfacial properties. The aim of this study was to investigate the effect of different placement time of RMGI (Resin Modified Glass Ionomer), composite resin and amalgam over different CSCs on the surface microhardness of these restorative materials.Methods: Each CSCs material (Biodentine, MTA, CEM cement) was mixed and carried into a hole (6mm diameter ×4 mm thickness) in the center of 270 molds (n=90 /each CSC). Then these molds were randomly divided into three main experimental groups (n=30) in regard of restorative materials (Amalgam, RMGI, Composite) that were placed in the other molds with the same hole size to make restorative materials and CSCs in contact together. Afterwards, each experimental group was divided into three subgroups according to the time interval of restoration placement that was immediately, after 24h and after 72h (n=10). Two molds were separated from each other after one week storage in incubator with 100% humidity in 37 °C, in order to evaluate the Vickers microhardness of restorative materials in CSC-restorative material interface. Statistical analysis included two-way Anova followed by Post hoc Dunnett T3 in cases with lack of homogeneity and Tukey HSD in cases with homogeneity.(p=0.05)Results: The microhardness of all restorative materials was neither significantly influenced by the CSCs materials (p>0.05) nor by the timing of final restoration (p>0.05) except in RMGI in immediate contact with CEM cement. (p<0.001)Conclusion: Restorative materials hardness in interface with studied CSCs may not affect. This in vitro study found no evidence against immediate definitive restoration over CSCs.
University Library System, University of Pittsburgh
2021-09-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/159
10.5195/d3000.2021.159
Dentistry 3000; Vol. 9 No. 1 (2021); 85-94
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/159/113
Copyright (c) 2021 Behnam Bolhari, Naghmeh Meraji, Pegah Khazaee, Sholeh Ghabraei, Sara Valizadeh
oai:ojs.dentistry3000.pitt.edu:article/161
2022-01-04T02:19:24Z
dentistry3000:DoCS
Durability of different types of Mouthwashes on the Salivary Buffering system in Orthodontic Patients.
R. Alkasso, Israa
Salih Al Qassar, Sarmad S.
Taqa, Ghada A.
Salivary pH
orthodontic appliance
Herbal mouthwash
Green tea mouthwash
Chlorhexidine mouthwash
The fast elevation of the salivary pH in orthodontic patients has acritical role to safe the teeth and encourage the orthodontic treatment after acidic challenger. As their baseline salivary pH is dropped during fixed orthodontic treatments.Aims: this study was aimed to evaluate the durability of three types of mouthwashes on the salivary buffering capacity in orthodontic patients at 0, 5, 10, 15 mints after acidic challenger using direct pH meter.Material and method: 80 orthodontic patients; age range (13-21years) were distributed into four groups; 20/ each, the salivary pH were recorded using compact pH meter (LAQUAtwin, HORIBA's, Kyoto, Japan) before and after administration of carbonated beverage (100 mm coca cola) and directly after gargling with 100 ml of the tested mouthwashes (herbal, green tea and chlorohexidine mouthwash) beside tap water as placebo control. The pH was documented again at 0, 5, 10 and 15 mins after each mouthwash gargling. One way ANOVA analysis and Duncan multiple were used for salivary pH analysis, p≤0.05.Results: non-significant differences were found at 0, 5, 10, 15 mints in comparison with baseline pH after herbal mouthwash gargling, the same result was shown with chlorohexidine mouth wash but with less defined border. Salivary pH after green tea gargling showed clear oscillatory results after the same tested periods.Conclusions: Herbal mouthwash is the best type to improve salivary pH for longer duration after acidic challenger. While Chlorohexidine mouthwash shows beneficial effects in elevation salivary pH with less duration. Green tea mouthwash fail to control salivary pH for long duration.
University Library System, University of Pittsburgh
2021-09-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/161
10.5195/d3000.2021.161
Dentistry 3000; Vol. 9 No. 1 (2021); 178-192
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/161/114
Copyright (c) 2021 Israa R. Alkasso, Sarmad S. Salih Al Qassar, Ghada A. Taqa
oai:ojs.dentistry3000.pitt.edu:article/164
2022-01-04T02:19:24Z
dentistry3000:DoCS
Differential expression of the IRF6 gene in the signs of Van der Woude Syndrome: Are distinct genetic modifiers operating?
Lisboa, Rosany de Oliveira
Martins, Cláudia Maria da Rocha
Santos, Maria Elisabete Silva
Pereira, Danilo Leôncio Aguiar
Carvalho, Flávia Martinez de
Orioli, Ieda Maria
Guerreiro, João Farias
Vieira, Alexandre Rezende
Santana-da-Silva, Luiz Carlos
Van der Woude syndrome
Orofacial clefts
IRF6 gene
Objective: The purpose of this study was to report a new variant in the Interferon Regulatory Factor 6 gene (IRF6) and to determine phenotype-genotype correlations in a family segregating Van der Woude syndrome.Methods: A five-generation family of 80 individuals segregating VWS was investigated using a tabulated pedigree but considering that three individuals registered in the pedigree died shortly after birth, the final sample size was 77 individuals. Five individuals had a complete dental clinical examination and molecular analysis performed using direct sequencing of the exon 4 and an adjacent region with 23 base pairs of the IRF6 gene.Results: Features of VWS reported in family history were present in 36.4% (28/77) of all family members; of these 57% (16/28) had pits in the lower lip, 36% (10/28) had both pits and orofacial clefts and 7 % (2/28) had only orofacial clefts. Developmental dental anomalies were observed in three individuals. The sequence analysis of exon 4 of the IRF6 gene carried out for 4 family members revealed the presence of the SNP rs7552506 (c.175-5C> G) located in five base pairs before the start of exon. The analysis of exon 4 of the IRF6 gene also revealed a new variant c.269G>C (p.Ser90Thr) which causes exchange of the Serine amino acid residue for the Threonine residue. Conclusions: The c.269G>C(p.Ser90Thr) can interfere with multimeric interactions and with protein conformation that will be slightly destabilized, because the mutant residue is bigger than the wild-type residue. The phenotypic variations in the cases studied, despite carrying the same genetic mutation, suggest that distinct genetic modifiers operate on the formation of clefts and dental development.
University Library System, University of Pittsburgh
2021-11-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Original research
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/164
10.5195/d3000.2021.164
Dentistry 3000; Vol. 9 No. 1 (2021); 193-204
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/164/118
Copyright (c) 2021 Rosany de Oliveira Lisboa, Cláudia Maria da Rocha Martins, Maria Elisabete Silva Santos, Danilo Leôncio Aguiar Pereira, Flávia Martinez de Carvalho, Ieda Maria Orioli, João Farias Guerreiro, Alexandre Rezende Vieira, Luiz Carlos Santana-da-Silva
oai:ojs.dentistry3000.pitt.edu:article/166
2022-01-04T02:19:24Z
dentistry3000:A%26tE
Effect of chlorhexidine, honey and Propolis on Streptococcus mutans Counts: in vitro Study
Alkhaled, Abdulrhman
Alsabek, Laith
Al-assaf, Maher
Badr, Faek
Streptococcus mutans
chlorhexidine
propolis
honey.
Objective: .Streptococcus mutans is considered one of the bacteria that causes necrosis, as it metabolism sugar to obtain energy, producing an acidic environment and causing depression in the enamel surface, which causes the dissolution of the calcium molecule, a problem of cavited in the tooth; so the aim of the research is to evaluate the effect of mouthwashes chlorhexidine 0.12% - honey 50% - propolis 5% on Streptococcus mutans in children.Study design: A laboratory study to assess the efficiency of chlorhexidine, honey and Propolis on Streptococcus mutans counts. The sample included 60 children divided into: G1 (Chlorhexidine 0.12% = 20 children), G2 (Propolis 5% = 20 children), G3 (Honey 100% = 20 children). The data was analyzed using the statistical analysis program SPSS, version 13.00, at a confidence level of 95% (P <0.05(. Paired sample T test was used to compare the variable means of the values of the studied variables.Results: There is significant difference between three groups. The reduction ratio was 89.97% in G1, 85.49% in G2 and 76.29% in G3.Conclusion: This study demonstrated the effectiveness of mouthwashes of chlorhexidine, propolis and honey on the bacteria of Streptococcus mutans.
University Library System, University of Pittsburgh
2021-11-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/166
10.5195/d3000.2021.166
Dentistry 3000; Vol. 9 No. 1 (2021); 107-117
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/166/119
Copyright (c) 2021 Abdulrhman Alkhaled, Laith Alsabek, Maher Al-assaf, Faek Badr
oai:ojs.dentistry3000.pitt.edu:article/172
2023-10-20T14:26:05Z
dentistry3000:A%26tE
Evaluation of Glenoid Fossa Morphology in Different Facial Growth Patterns: A Cone Beam Computed Tomography Study
Karandish, Maryam
Farmani, Somaye
Khademi, Bahar
Paknahad, Maryam
Glenoid Fossa
Growth Pattern
CBCT
TMJ
Purpose: The aim of current study was to assess the glenoid fossa morphology among different vertical skeletal patterns using cone beam computed tomography (CBCT).Materials and Methods: The CBCT images of 63 patients with Class I sagittal skeletal pattern were classified into three groups based on posterior facial height (PFH)/ Anterior facial height (AFH) ratio. Each group divided in to normal, horizontal and vertical growth pattern groups. Depth, width and inclination of glenoid fossa were measured and assessed on CBCT images. One-way analysis of variance (ANOVA) and Tukey post-hoc test were used to compare mean values of measured variables among the groups.Results: The width (28.76±0.79) and depth (14.61±0.514) of glenoid fossa were lower in horizontal in comparison to vertical and normal growth patterns (p-value<0.05). Although steepness of glenoid fossa was higher in normal growth pattern (123.8±16.68), no statistically significant differences were seen (p-value: 0.819).Conclusion: The width and depth of glenoid fossa were significantly lower in horizontal growth pattern.
University Library System, University of Pittsburgh
2023-01-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/172
10.5195/d3000.2023.172
Dentistry 3000; Vol. 11 No. 1 (2023)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/172/169
Copyright (c) 2023 Maryam Karandish, Somaye Farmani, Bahar Khademi, Maryam Paknahad
oai:ojs.dentistry3000.pitt.edu:article/176
2022-05-02T17:23:56Z
dentistry3000:I%26A
Tridimensional roughness and morphology of sound dentin surfaces after papain-gel treatment
Pires, Paula Maciel
Miranda, Paula Martins Bravo
Costa, Paula Helena de Accioly
Monteiro, Amanda Souza Nunes
Alexandria, Adílis Kalina
Cople Maia, Lucianne
Neves, Aline de Almeida
dental caries
chemomechanical caries removal
dental materials
dentin
papain
pediatric dentistry
Purpose: To investigate the effect of chemomechanical caries removing agents (CCRAs) based on papain (Papacárie Duo Gel® and Brix3000®) over dentin surfaces compared with 37% phosphoric acid and 11.5% polyacrylic acid.Materials and methods: Sound human molars were sectioned at the crown level, resulting in 48 dentin blocks, which were randomly divided into 4 groups (n=12): 1) Papacárie Duo Gel®; 2) Brix3000®; 3) 11.5% polyacrylic acid solution and 4) 37% phosphoric acid gel. All products were applied for 30s. Ten blocks per group were analyzed by a non-contact 3D profilometer before and after treatments for linear (Ra) and volumetric roughness (Sa). The superficial morphology of the remaining blocks in each group (n=2) was evaluated by scanning electron microscopy (SEM). Normality was rejected for the data (Shapiro-Wilk test) and therefore, Kruskal-Wallis test, followed by Dunn’s proof or Wilcoxon signed rank test with its respective effect size calculation were used to compare the results with α=5%.Results: Ra and Sa values for specimens submitted to Papacárie Duo Gel® and Brix3000® were statistically similar to baseline values. Application of phosphoric and polyacrylic acid resulted in a statistically increase in roughness compared to the CCRAs. SEM evaluation showed that Papacárie Duo Gel® resulted in surface debris. Polyacrylic acid and Brix3000®resulted in partial opening of the tubules but dentin exposed to polyacrylic was able to remove more smear layer than Brix3000®, while phosphoric acid resulted in total opening of the dentinal tubules.Conclusion: Both Papacárie Duo Gel® and Brix3000® did not result in roughness changes when applied in sound dentin.
University Library System, University of Pittsburgh
2022-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
in vitro
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/176
10.5195/d3000.2022.176
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/176/134
Copyright (c) 2022 Paula Maciel Pires, Paula Martins Bravo Miranda, Paula Helena de Accioly Costa, Amanda Souza Nunes Monteiro, Adílis Kalina Alexandria, Lucianne Cople Maia, Aline de Almeida Neves
oai:ojs.dentistry3000.pitt.edu:article/178
2022-03-21T12:49:52Z
dentistry3000:MoOD
Use of Fonseca’s Questionnaire to assess the prevalence and severity of Temporomandibular disorders among university students - a cross sectional study
G Shivamurthy, Prashantha
Kumari, Nutan
Sadaf, Ashfiya
MB, Meghana
Azhar, Heba
Sabrish, Sharanya
Temporomandibular joint dysfunction
Fonseca’s questionnaire
Stress
Aim: Temporomandibular disorders (TMD) are one of the main causes of non-dental orofacial pain. It has been reported that TMD occurred more frequently among individuals in stressful situations and that university students are more likely to have a high risk of anxiety related disorders. This study aims to assess the prevalence and severity of TMD in undiagnosed population consisting of students of different faculties in a university campus in Bengaluru using the Fonseca’s questionnaire.Material and Methods: Descriptive cross-sectional study conducted at a University Campus, Bengaluru. Completed forms of Fonseca’s questionnaire were collected from the sample consisted of 300 students. The questionnaire had two main parts. First part collected demographic information and past relevant histories. Second part had Fonseca’s 10 questions. The TMD severity was categorized as no TMD (0-15), mild TMD (20-40), moderate TMD (45-65) and severe TMD (70-100).Results: 33.3% on the sample showed varying signs of TMD. There was no statistically significant differences found between the TMD and non TMD groups with respect to age and sex. But when comparing varying grades of TMD there was a statistically highly significant difference seen in relation to age of the subjects.Conclusions:A high prevalence of TMD was found in the university students with majority of the cases having milder signs of TMD.
University Library System, University of Pittsburgh
2022-02-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
cross sectional study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/178
10.5195/d3000.2022.178
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/178/122
Copyright (c) 2022 Prashantha G Shivamurthy, Nutan Kumari, Ashfiya Sadaf, Meghana MB, Heba Azhar, Sharanya Sabrish
oai:ojs.dentistry3000.pitt.edu:article/179
2022-03-21T12:49:52Z
dentistry3000:A%26tE
Comparative evaluation of dimensional stability and flexural strength of different light cured tray materials with self-cured tray material: an in-vitro study
Attarwala, Dr Taha
Dugal, Dr Ramandeep
Godil, Dr Aamir
Wadwan, Dr Sanaa
Madanshetty, Dr Pallavi
Kazi, Dr Arshi
Custom trays
light cured resins
dimensional stability
flexural strength
Objectives: To carry out a comparative evaluation of dimensional stability and flexural strength of different light cured tray materials with self-cured tray material as control.Materials and methods: The study was carried out in four groups based on the different available tray materials. Group A (n=30) – light cured resin (Individo Lux-VOCO™ GmbH Germany); Group B (n=30)- light cured resin (Profibase-VOCO™ GmbH Germany); Group C (n=30)- light cured resin (Plaque Photo-Willmann and Pein™ GmbH Germany); Group D (n=30)- control group-self cured resin (Asian special instant tray material Asian Acrylates™, Mumbai, India). A digital micro-meter was used to measure the length of each specimen first at 1 hour, 24 hour and 48 hour and three readings were taken up to 0.001 decimal. The values for flexural strength of each specimen were measured using the 3-point bending technique with the help of a universal testing machine. The mean values of dimensional stability and flexural strength were calculated by the one-way ANOVA and Tukey’s post-hoc test for multiple group`s comparison.Results The mean change in length (in mm) of light cured resins samples were significantly lower than self-cured resin samples at all three time intervals. The flexural strength values of light cured resin samples-Group A (84.46±13.32 N/mm2), B (83.43±14.52 N/mm2) and C (86.80±14.73 N/mm2) was significantly higher than the self-cured resin samples group D (41.29±7.93 N/mm2)Conclusions: Light cured tray materials are more dimensionally stable and have a higher flexural strength compared to self-cured tray materials.
University Library System, University of Pittsburgh
2022-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Original Research, in-vitro study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/179
10.5195/d3000.2022.179
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/179/130
Copyright (c) 2022 Dr Taha Attarwala, Dr Ramandeep Dugal, Dr Aamir Godil, Dr Sanaa Wadwan, Dr Pallavi Madanshetty, Dr Arshi Kazi
oai:ojs.dentistry3000.pitt.edu:article/183
2022-06-14T20:02:28Z
dentistry3000:A%26tE
A Study on Patient Behaviors Towards Dental Visits and Oral Health during COVID-19 Outbreak
Mahmood, Marriam
COVID-19
dentistry
patient comfort
oral health.
Background Investigating the impact of COVID-19 pandemic on oral health and patient attitudes towards a dental visit is essential to improving access to oral health care during the pandemic. This calls for studying the chief patient fears, preferences and future intentions related to dental visits during the COVID-19 pandemic.MethodsA web-based survey was administered to the public within the US in December 2020. The participants (N=502) answered questions about their oral health, trust level for dental offices, chief concerns and preferences related to dental visits during the COVID-19 outbreak. We used descriptive statistics (e.g., Chi-square, Shapiro-Wilk, Kruskall-Wallis and Wilcoxon Signed Rank tests) to find if demographics, oral health history or social behaviors were associated with the relevant patient attitudes. ResultsWhen queried about COVID-19’s impact on oral health, 62.5% respondents reported a minimal impact, 7.2% a positive impact, and 30.3% a negative impact. There were statistically significant differences in responses based on respondents’ ethnicity (N=502, P=.033) and dental visit history during the pandemic (N=502, P=.008).The public trust rating for COVID-19-related precautions was more favorable towards the medical offices than the dental offices (N=502, P<.001). The majority considered contracting COVID-19 from the other patients in the dental office waiting area (60.4%), the dentist/hygienist/dental assistants (54.2%), and the aerosols (50.8%) as their chief concerns. 20.1% respondents preferred no other patient, 25.1% only one more patient, and 31.1% up to three more patients in the waiting area during a dental visit.ConclusionsThe reported impact of COVID-19 on self-perceived oral health was modest for most respondents. Patients’ chief concerns for a dental visit include contracting COVID-19 infection from other patients, dentists, and aerosols in the operatory. It may be beneficial if dental practices avoid scheduling multiple patients in the waiting area to improve access to oral health care.
University Library System, University of Pittsburgh
2022-06-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/183
10.5195/d3000.2022.183
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/183/142
Copyright (c) 2022 Marriam Mahmood
oai:ojs.dentistry3000.pitt.edu:article/184
2023-10-20T14:26:06Z
dentistry3000:A%26tE
Wettability of two different artificial saliva substitutes on injection moulded heat polymerized acrylic resin and CAD-CAM acrylic resin: an in vitro study.
Lal, Quraish
Godil, Aamir
Shaikh, Mosin
Musani, Smita
Dugal, Ramandeep
Kirad, Ajinkya
wettability
artificial saliva substitute
CAD-CAM acrylic resin
injection moulded acrylic resin
contact angle
Mouth Kote
Wet mouth.
Aim: The purpose of this in vitro study was to evaluate the wettability of two different artificial saliva substitutes on injection moulded heat-polymerized acrylic resin and CAD-CAM acrylic resin and to compare these properties with natural saliva and distilled water.Materials and method: Forty heat polymerized (injection moulded) acrylic resin specimens and forty CAD-CAM acrylic resin specimens were fabricated with each specimen measuring 10x10x2mm. Four groups of liquids namely distilled water, Wet mouth™, Mouth Kote™ and human saliva were used. A contact angle goniometer was used to measure the contact angles. Two contact angle readings were obtained on the right side and on the left side of the test specimen. The observations were recorded and a mean of the two readings was calculated. The same procedure was repeated for all specimens (heat cure resin and CAD-CAM) with all the four test groups of liquids.Results: Contact angle measurements were significantly higher in Wet mouth™ and Mouth Kote™ solution of heat cure resin group compared to the CAD-CAM group (p-value<0.05 for both). Mouth Kote™ had lower mean contact angle (62.63 ± 4.59) than Wet mouth™ (77.46 ± 9.16) on CAD/CAM denture base resin. Mouth Kote also had lower mean contact angle (70.80 ± 4.79) than Wet mouth™ (97.77 ± 7.79) on injection moulded denture base resin.Conclusion: Mouth kote had better wettability than wet mouth on both CAD-CAM and injection moulded acrylic resin. Both the saliva substitutes had better wettability on CAD-CAM than on injection moulded acrylic resin.
University Library System, University of Pittsburgh
2023-02-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
in vitro study
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/184
10.5195/d3000.2023.184
Dentistry 3000; Vol. 11 No. 1 (2023)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/184/173
Copyright (c) 2023 Quraish Mustafa Lal
oai:ojs.dentistry3000.pitt.edu:article/185
2022-05-02T17:23:56Z
dentistry3000:I%26A
Social Isolation in COVID-19: Impact on Mental Health of Young Adults
Verma, Aditi
Mehta, Abhishek
Background: The coronavirus disease (COVID-19) is profoundly affecting life around the globe. Social Isolation, contact restrictions and economic shutdown impose a complete change to the psychosocial environment threatening the mental health of young adults significantly.Objectives: The present study aims to assess the impact of Social Isolation on the mental health of young adults during the pandemic.Methods: A web based research was conducted on a sample of 438 college students of age 18-24 years. The participants were asked for responding to a standardized online questionnaire that included questions related to their anxiety levels and the 7-item Generalized Anxiety Disorder Scale (GAD-7).Results: The results revealed 78.4% of the study populations were suffering with mild (35.2%), moderate (23.7%), and severe anxiety (19.4%) respectively. Univariate and Logistic Regression Analysis revealed gender and course/year of study had a significant effect on the level of anxiety (p<0.05). Also, students living alone and having a relative or an acquaintance infected with COVID-19 were likely to be more anxious (p < 0.05). Spearman correlation analysis depicted Social Isolation as the main Covid-19 related stressor which was positively correlated and highly significant (p=0.0001) to the level of anxiety of young adults.Conclusions: The present study revealed approximately 43% of young adults is suffering from moderate to severe anxiety levels which require urgent attention.
University Library System, University of Pittsburgh
2022-04-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/185
10.5195/d3000.2022.185
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/185/135
Copyright (c) 2022 Aditi Verma, Abhishek Mehta
oai:ojs.dentistry3000.pitt.edu:article/189
2022-06-14T20:02:28Z
dentistry3000:I%26A
The Effect of Using an Educational Poster on Mothers’ Knowledge of Emergency Management of Dental Avulsion
Daneshvar, Seyedeh Hediyeh
Khaef, Melika
Tooth Avulsion
Knowledge
Pediatric Dentistry
Introduction: Mothers are commonly the first ones who encounter the dental avulsion in children. High knowledge of them could greatly affect the success rate of treatment. The purpose of this study was to evaluate the effect of educational poster on the mother’s knowledge about management of dental avulsion.Materials and Methods: This study was conducted at the Department of Pediatric Dentistry, Guilan University of Medical Sciences from November 2020 to February 2021. After obtaining the informed consent, a questionnaire containing the mothers’ socio-demographic information and 8 questions regarding knowledge about dental avulsion was responded alone by 262 mothers (131 in the study group and 131 in control group) before educational poster presentation in the study group. After three months, the participants of both groups were re-evaluated using the same questionnaire. McNemar’s test and Generalized Estimating Equations (GEE) was used to analyze the data. Results with P < 0.05 were considered statistically significant. Results: Use of an educational poster improved the knowledge of participants of study group about dental avulsion, although this improvement was statistically significant in questions regarding immediate emergency action (P=0.003), appropriate time for replantation (P<0.001), cleaning before replantation of a dirty tooth(P=0.022) and suitable storage medium for transferring an avulsed tooth (P<0.001). It was found that variables like age, level of education, employment status and previous training had no significant effect on participants knowledge(P>0.05).Conclusion: Results showed that the use of posters as an educational method can effectively improve mothers’ knowledge about management of children’s dental avulsion.
University Library System, University of Pittsburgh
2022-05-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/189
10.5195/d3000.2022.189
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/189/138
Copyright (c) 2022 Seyedeh Hediyeh Daneshvar, Melika Khaef
oai:ojs.dentistry3000.pitt.edu:article/194
2022-06-14T20:02:28Z
dentistry3000:A%26tE
Impact of COVID-19 on the frequency of dental appointments and types of dental health care services
Alshami, Muhanad L.
Abdulkareem, Ali A.
Gul, Sarhang S.
Shareef, Yasir A.
COVID-19
pandemics
impact
dental care
dental health services
Objectives: To investigate the impact of the COVID-19 pandemic on the frequency of provision of dental appointments and types of dental care services.Methods: The dental records of three dental health services, over 3 months, in the year before (2019) and a year during (2020) the pandemic were retrieved. The dental records were categorized according to the referral after diagnosis into prosthodontics, pedodontics, orthodontics, conservative, endodontics, periodontics, and oral surgery. The demographic variables and types of treatment provided were recorded and analyzed. The frequency of appointments was determined from overall dental records and departmental dental records. Chi square test was used to determine the effect of gender and age on dental appointments.Results: According to the dental records for 2019, 7286 appointments were provided, and this number decreased by 2.4-fold to 3090 appointments in 2020. The records indicated that the highest decreases were in non-urgent dental appointments in the periodontics, orthodontics and conservative departments (8, 8, and 4.6-fold, respectively). Statistically significant differences in dental pre-pandemic and pandemic appointments were identified for gender and age group. Female and older (>64 years old) subjects appeared to be less willing to attend dental appointments (OR=1.2 and 1.3, respectively) compared to their male and younger counterparts.Conclusion: The COVID-19 pandemic has had significant influence on decreasing attendance at dental appointments and the records show that the highest decreases were in non-urgent dental departments.
University Library System, University of Pittsburgh
2022-05-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/194
10.5195/d3000.2022.194
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/194/136
Copyright (c) 2022 Ali A Abdulkareem
oai:ojs.dentistry3000.pitt.edu:article/205
2022-01-10T21:39:31Z
dentistry3000:MoOD
Immunohistochemical expression of Cyclooxygenase 2 reflects the proliferative activity in the epithelium of odontogenic lesions
Verma, Vani
Chandrashekar, Chetana
Radhakrishnan, Raghu
Solomon, Monica Charlotte
Cysts
COX-2
aggressive
inflammatory
prognosis
Purpose: Odontogenic cysts and tumors comprise a major component of lesions of the oral and maxillofacial region. The pathogenesis of these lesions involves the interaction between the odontogenic epithelium and the ectomesenchyme. However, the clinical behavior of these biological entities is unpredictable. The aim of this study was to evaluate the role of Cyclooxygenase 2 (COX-2) in the pathogenesis and prognostication of odontogenic lesions.Material and method: : In this study formalin-fixed paraffin-embedded tissue section of Odontogenic Keratocyst (n=10) Dentigerous cyst (n=10), Radicular cyst (n=10) and unicystic ameloblastoma (n=10) were immunohistochemically stained with COX-2 (NCL2-COX-2- 4H12) and with Ki 67 (Ki-67 GM001) using standard staining protocols. The cytoplasmic expression of COX-2 in all the lesions was semi-quantitatively assessed. The pattern of expression of COX-2 among the different odontogenic lesions was statistical analyzed using the ANOVA test and the chi-square test.Results: All the 40 odontogenic lesions that were evaluated expressed COX-2 immunohistochemically. A high number of odontogenic epithelial cells expressed COX-2 in most of the odontogenic keratocyst, radicular cyst and unicystic ameloblastomas. The expression of COX-2 was significantly (p=0.036) higher in Unicystic Ameloblastomas and Radicular cyst compared to that of Odontogenic Keratocyst and the dentigerous cyst.Conclusion: The recognition that expression of COX-2 by odontogenic epithelial cells may indeed shed a new light on the biological mechanisms involved in the development of these benign yet aggressive lesions of the jaws. An insight into the molecular interactions occurring in the odontogenic epithelium will aid in better management of these lesions.
University Library System, University of Pittsburgh
2022-01-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Immunohistochemical
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/205
10.5195/d3000.2022.205
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/205/121
Copyright (c) 2022 Vani Verma, Chetana Chandrashekar, Raghu Radhakrishnan, Monica Charlotte Solomon
oai:ojs.dentistry3000.pitt.edu:article/211
2022-01-04T02:19:24Z
dentistry3000:ER
Erratum to: Effects of a novel dye containing toothpaste versus regular fluoridated toothpaste for supragingival biofilm reduction in patients with chronic gingivitis: A Randomized Controlled Trial. Rath, A., Chong, J.N., HR, P., Teo, B., Zhen, N., & Yin, O. (2021). Dentistry 3000 2021; 9(1). DOI: https://doi.org/10.5195/d3000.2021.117
Rath, Avita
The name of one of the authors was misspelled by the author during the submission process as well as a name of a collaborator in the acknowledgement section. The author’s name Chong Junning was corrected to Chong Jun Ning. In the acknowledgment section Mr. Che Vern was corrected to Mr. Yeoh Chin Vern. The original manuscript has been updated to reflect the aforementioned changes.
University Library System, University of Pittsburgh
2021-07-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/211
10.5195/d3000.2021.211
Dentistry 3000; Vol. 9 No. 1 (2021)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/211/104
Copyright (c) 2021 Avita Rath
oai:ojs.dentistry3000.pitt.edu:article/216
2022-06-14T20:02:28Z
dentistry3000:A%26tE
Alveolar ridge augmentation in mandible by use the modified distractor.
Sulaiman, Feras
Alkhouri, Issam
Al-assaf, Maher
horizontal alveolar
vertical alveolar
distraction osteogenesis
distractor
Objective: The aim of the present study was to evaluate the horizontal and the vertical alveolar distraction osteogenesis using modified alveolar distractor.Study design: The sample consisted of 7 patients presenting horizontally and vertically deficient edentulous ridges were treated by distraction osteogenesis with a modified Alveolar distractor. The surgical procedure was carried out with the patient under local anesthesia. After the osteotomy was performed with pizosurgery, the distractor was placed on the segmental bone over the gingiva for 3 months. The rate of distraction was 0.5 mm/twice a day.Results: The mean of horizontal alveolar before using the distractor was 4.70 mm (SD 0.31 mm) The mean of horizontal alveolar after using the distractor was 8.30 mm (Sd 0.59 mm). The mean of actual gain in bone horizontal at the end of the distraction period was 3.58 mm (SD 0.15 mm). The mean of height alveolar before using the distractor was 6.67 mm (SD 0.46 mm) The mean of height alveolar after using the distractor was 12.59 mm (SD 0.52 mm). The mean of actual gain in bone height at the end of the distraction period was 5.92 mm (SD 0.25 mm).Conclusion: It was concluded that the modified alveolar distractor seems to be an effective to treat horizontal and vertical alveolar ridge deficiencies, and distraction osteogenesis can be considered a safe and effective procedure for gaining bone in the horizontal and the vertical dimension of the alveolar.
University Library System, University of Pittsburgh
2022-05-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/216
10.5195/d3000.2022.216
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/216/137
Copyright (c) 2022 Feras Sulaiman, Issam Alkhouri, Maher Al-assaf
oai:ojs.dentistry3000.pitt.edu:article/225
2022-06-14T20:02:28Z
dentistry3000:MoOD
Effect of aloe vera hydrogel on the scarring and healing of free gingival graft: Randomized controlled trial
Alawad, Walaa
Othman, Majed
Hsaian, Jameela
Alsabek, Laith
free gingival graft
aloe vera
hydrogel
scarring
Objective: Several studies have used aloe vera hydrogel to enhance various tissues regeneration to reduce scar formation in wounds. This study aimed to prepare a hydrogel specified for the oral cavity and observe scar formation and healing of free gingival graft after its application compared with the natural healing and scaring of the graft without any material.Design: This study is a randomized controlled clinical trial that included 22 patients with a width of attached gingiva less than 2 mm. after preparing the aloe vera hydrogel and completing the laboratory stage, the hydrogel was randomly applied for 11 patients after the free gingival graft procedure while the other 11 patients did not use any material after the same surgery. Landry’s scale was used to assess the healing index, scarring was evaluated using Mucosal Scarring Index.Results: The healing index was higher in the intervention group with a statistical difference P<0.05 than the control group after three weeks. Moreover, Scar formation in the intervention group was less in the intervention group with a significant difference P<0.05 after 1,2,3,6 months.Conclusion: Within the limitations of our study, the aloe vera hydrogel can be considered as a good topical treatment after oral surgery due to its ability to reduce scar formation and speeding up the healing in the receiving area in free gingival graft procedures.
University Library System, University of Pittsburgh
2022-05-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/225
10.5195/d3000.2022.225
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/225/139
Copyright (c) 2022 Walaa Alawad, Majed Othman, Jameela Hsaian, Laith Alsabek
oai:ojs.dentistry3000.pitt.edu:article/237
2022-06-14T20:02:28Z
dentistry3000:DoCS
Hypertrichosis associated with genetic conditions with head and neck alterations
Caixeta, Mirelle Estéfane de Oliveira
Dias, Caroline Rodrigues
Pereira, Rafael Martins Afonso
Carvalho, Thiago de Amorim
Silva, Ivania Aparecida Pimenta Santos
Andrade, Rodrigo Soares de
Craniofacial Abnormalities
Dermatology
Genetic Disorders
Hirsutism
Hypertrichosis
INTRODUCTION: Hypertrichosis is a rare disorder in which there is an exaggerated growth of body hair in places that are not necessarily androgen-dependent, a particular feature of hirsutism. Its etiology is still questionable, with an increased incidence when associated with syndromes. This genetic association leads to numerous significant systemic and craniofacial changes, which should be recognized and considered by the dentist.OBJECTIVE: To assess, in databases, the presence of congenital hypertrichosis associated with genetic syndromes and conditions affecting the head and neck.METHODS: The search was conducted in PubMed-NCBI databases; genetic conditions along with their characteristics were available in Online Mendelian Inheritance in Man (OMIM) and in Protein®.RESULTS: The analysis was performed on 63 articles and all diseases were considered as rare. In the syndromes, prevalence of autosomal dominant inheritance was observed in 48.64%, followed by autosomal recessive in 45.9%, 1 X-linked recessive case, and 1 arising from defective mitochondrial energy generation. Among the 63 genetic conditions, 44 showed neurodevelopmental changes. Besides the craniofacial involvement itself, there is a high prevalence of alterations of the oral structures involving 47 of all conditions, including mainly dental abnormalities, palatal and gingival changes.CONCLUSION: The association of hypertrichosis with genetic components is strongly associated with major craniofacial changes. Therefore, the knowledge of the dental surgeon about the conditions that can affect the oral cavity is impressive because it is related to a correct treatment and better quality of life for the patient.
University Library System, University of Pittsburgh
2022-06-14
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/237
10.5195/d3000.2022.237
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/237/143
Copyright (c) 2022 Mirelle Estéfane de Oliveira Caixeta, Caroline Rodrigues Dias, Rafael Martins Afonso Pereira, Thiago de Amorim Carvalho, Ivania Aparecida Pimenta Santos Silva, Rodrigo Soares de Andrade
oai:ojs.dentistry3000.pitt.edu:article/238
2022-08-12T17:38:45Z
dentistry3000:DoCS
Use and effectiveness of the Failure Modes and Effects Analysis (FMEA) for identification of potential errors and failures in the process of root canal treatment
Yazdanian, Mohsen
Alam, Mostafa
Rafiee, Esmail
Tahmasebi, Elahe
Ghaffarpasand, Arash
Abbasi, Kamyar
Bahadori, Mohammadkarim
Dentistry
Endodontics
Failure Modes and Effects Analysis
Risk Priority Number
Patient Safety
Human Errors
Background and Aim: Root canal treatment (RCT) has high technical sensitivity, and many human procedural errors can compromise the success of treatment and well-being of patients. The failure modes and effects analysis (FMEA) is a risk assessment and management tool that ensures the safety of patient care by a systematic approach to failures. This study aimed to identify the potential errors and failures in the process of RCT using the FMEA.Materials and Methods: This descriptive study evaluated the failure modes and their effects qualitatively and quantitatively using the FMEA methodology. For this purpose, a FMEA team was first established and the steps of RCT were described in a flowchart. Next, the potential errors and failures in each step were identified, and each failure mode was scored from 1 to 10, based on the severity of impact, likelihood of occurrence, and likelihood of detection. The three scores were multiplied to obtain the risk priority number (RPN). Corrective measures and preventive strategies were suggested for high-risk failure modes (RPN≥250).Results: The FMEA identified 19 steps and 48 potential failures in the process of RCT. The maximum RPN in the process of RCT was assigned to file fracture in the root canal (RPN=324), apical extrusion of irrigating solution (RPN=320), and inappropriate or no rubber dam isolation (RPN=315).Conclusion: The FMEA has high efficacy for detection and prioritization of improvable points in a complex dental procedure in a busy department such as the endodontics department.
University Library System, University of Pittsburgh
2022-08-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/238
10.5195/d3000.2022.238
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/238/159
Copyright (c) 2022 Mohsen Yazdanian, Mostafa Alam, Esmail Rafiee, Elahe Tahmasebi, Kamyar Abbasi, Mohammadkarim Bahadori
oai:ojs.dentistry3000.pitt.edu:article/245
2023-10-20T14:26:06Z
dentistry3000:DoCS
Evaluation of Tongue Volume and Airway Volume in Skeletal Class I and Class II Patients using Cone Beam Computed Tomography- A Cross Sectional Study
Thomas Abraham, Jim
Sagarkar, Roshan M
Sabrish, Sharanya
Govinakovi Shivamurthy, Prashantha
Mathew, Silju
Rakesh, Nagaraju
airway volume
Class I skeletal malocclusion
Class II skeletal malocclusion
Dolphin software
tongue volume
BACKGROUND: The volumetric relationship between the tongue and the oral cavity is an important factor in tooth alignment and occlusion. The aim of this study was to evaluate the difference in tongue and airway volume in skeletal Class I and Class II malocclusion.
MATERIALS AND METHODS: The CBCT data which comprised of 30 skeletal Class I and 30 skeletal Class II was stored in DICOM format. Tongue volume and airway volume was analyzed with the help of 3D dolphin software. Values obtained were subjected to statistical analysis with help of SPSS.
RESULTS: Skeletal Class I pattern had more tongue volume with a mean of 45.32 mm3 than skeletal Class II pattern with mean value of 40.27mm3 with P value of <0.001. Skeletal Class I pattern has more airway volume with a mean of 15058.6 mm3 than patients with Skeletal Class II pattern with mean value of 12458.8mm3 with a P value of 0.003. A P value of 0.001 indicated that there is a strong correlation between the tongue volume and airway volume in both skeletal Class I and skeletal Class II subjects.
CONCLUSION: The volume of tongue and airway was greater in skeletal Class I malocclusion compared to skeletal Class II malocclusion. There exists a positive correlation between the tongue volume and airway volume in both the skeletal patterns.
University Library System, University of Pittsburgh
2023-10-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/245
10.5195/d3000.2023.245
Dentistry 3000; Vol. 11 No. 1 (2023)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/245/185
Copyright (c) 2023 Jim Thomas Abraham, Roshan M Sagarkar, Sharanya Sabrish, Prashantha GS, Silju Mathew, Rakesh N
https://creativecommons.org/licenses/by/4.0
oai:ojs.dentistry3000.pitt.edu:article/254
2022-06-21T18:38:58Z
dentistry3000:DoCS
Association of sella turcica bridging and morphologic characteristics with palatally impacted canine in lateral cephalograms
Ghasemi, Nikoo
Akhoondi, Soraya
Reyhani, Zohre
Motamed, Nima
Nourian, Azin
Tooth abnormalities
Orthodontics
Sella turcica
Introduction: Researchers have been interested in the relationship between sella turcica bridging and various dental anomalies. This study investigates the association of sella turcica bridging and morphologic characteristics with the palatally impacted canine tooth.Materials and Methods: Orthodontic records with high-quality lateral cephalometric and panoramic images were chosen. The study comprised 30 patients with palatally impacted canines (17 females and 13 males) and 30 controls with erupted canines (15 males and 15 females). The measurements were performed using the SCANORA 5.2.6 software. Further, the relevant data was collected and analyzed using SPSS software version 22. Descriptive statistical analysis was used to investigate data after their validation, with t-tests and chi-square tests to compare the two groups. The regression test was used to lessen the effects of confounding variables.Results: In the study group, 5 patients had type III sella turcica bridging, whereas 2 patients in the control group had type III sella turcica bridging. Results showed a statistically significant difference between the two groups (P-value=0.017). In the study group, the interclinoid distance and canine tooth angle were significantly lower than the control group (P-value=0.001, P-value=0.001). However, there were no significant differences in the depth and anterior-posterior diameter of sella turcica between the two groups (P-value=0.543, P-value=0.766). In terms of age (P-value = 0.400) and gender (P-value = 0.605), there was no significant difference between the two groups.Conclusions: The palatally canine impaction may be associated with the bridging of the sella turcica. Therefore, sella turcica bridging can be used as a diagnostic parameter for palatally canine impaction.
University Library System, University of Pittsburgh
2022-06-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/254
10.5195/d3000.2022.254
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/254/144
Copyright (c) 2022 Nikoo Ghasemi, Soraya Akhoondi, Zohre Reyhani, Nima Motamed, Azin Nourian
oai:ojs.dentistry3000.pitt.edu:article/258
2022-07-08T15:12:15Z
dentistry3000:DoCS
The influence of IGF-I and Emdogain on the behavior of dental epithelial cells in a three-dimensional scaffold model
Heezen, Maria Johanna Jacoba
Zhang, Weibo
Rudolph, Sara
Walboomers, X. Frank
Yelick, Pamela C.
Tissue engineering
Dental epithelial/mesenchymal cells
scaffold
IGF-1
Emdogain
Aim: Tooth decay and associated periodontal disease remain the most common chronic diseases in current society. In the future, individually tailored, more effective therapeutic treatment options could be provided through the application of tissue engineering and regenerative medicine and dentistry (TERMD) approaches. A relevant finding is the potential of both dental mesenchymal and dental epithelial cells to regenerate mineralized dentin and enamel tissues, respectively.Materials and Methods: In the current study, a multi-layered, bioengineered tooth bud model was assembled by combining human dental mesenchymal (hDM) and porcine dental epithelial cells (pDE). The hDM cells were seeded onto poly(epsilon-caprolactone)/ poly (lactide-co-glycolide) (PCL/PLGA) wet electro-spun scaffolds overlayed with confluent pDE cell sheet harvested from thermo-reversible tissue culture plates. The so-formed multi-layered bioengineered tooth bud was then used to study the mineralization potential of the dental cells in in vitro culture. It was hypothesized that the addition of the in media soluble factors Insulin-like growth factor 1 (IGF-I) and the extracellular matrix derivate Emdogain (EMD) would result in enhanced differentiation and mineralized dental tissue formation. Scanning Electron morphological observation was used to characterize scaffolds porosity. Histological and immunofluorescent analyses confirmed the localization of hDM cells inside the scaffold, an intact pDE cell sheet, and the presence of beta-integrin 1-positive cell-cell junctions connecting the two.Results: Scanning Electron Microscopy showed that EMD, in particular, enhanced the mineralization potential of pDE cells. qRT-PCR analyses showed that both EMD and IGF-1 significantly enhanced the expression of Ameloblastin (AMBN), reflecting pDE cell differentiation.Conclusion: In conclusion, these results proved the hypothesis that both EMD and IGF-1 should be considered for their utility in preclinical dental tissue engineering approaches.
University Library System, University of Pittsburgh
2022-07-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research Article
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/258
10.5195/d3000.2022.258
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/258/145
Copyright (c) 2022 Maria Johanna Jacoba Heezen, Weibo Zhang, Sara Rudolph, X. Frank Walboomers, Pamela C. Yelick
oai:ojs.dentistry3000.pitt.edu:article/267
2022-07-13T18:35:15Z
dentistry3000:MoOD
In-Vitro antibacterial activity of glass ionomer cements containing silver nanoparticles synthesized from leaf extract of Mentha piperita
Goldani Moghadam, Mahdjoube
Bagherzade, Aylin
Ghorbanzade, Fateme
Hanafi-Bojd, Mohammad Yahya
Yousefi, Masoud
Antibacterial activity
Glass ionomer cement
Mentha piperita
Silver nanoparticles
Purpose: The aim of this study was to evaluate the antibacterial activity of glass ionomer cement incorporated with silver nanoparticles (AgNPs) synthetized using mint leaf extract (Mentha piperita, M. piperita) on some oral cavity bacteria.Materials and methods: In the present study, M. piperita leaf extract was used for the synthesis of AgNPs. A total of 60 glass ionomer cement (GIC) disk-shaped specimens were prepared and divided into two groups: conventional GIC (C-GIC), and glass ionomer cement with 2 wt% AgNPs (GIC-AGNPs). The antibacterial activity of the GIC specimens in comparison with Ampicillin disk (10 µg/ml) was investigated against Streptococcus mutans, Enterococcus faecalis, Lactobacillus acidophilus, Lactobacillus casei, and Streptococcus aureus by measuring the diameter of growth inhibition zones.Results: C-GIC specimens failed to show any antibacterial effect against the studied bacteria. However, the GIC-AgNPs had relatively significant antibacterial effects on S. mutans, L. acidophilus, L. casei and S. aureus. The highest antibacterial effect of GIC-AgNPs specimens was reported against L. acidophilus (P <0.001). GIC-AgNPs had no antibacterial effect on E. faecalis.Conclusion: Glass ionomer cement incorporated with AgNPs synthetized using M. piperita showed a promising antibacterial effect against oral cariogenic pathogens.
University Library System, University of Pittsburgh
2022-07-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/267
10.5195/d3000.2022.267
Dentistry 3000; Vol. 10 No. 1 (2022)
2167-8677
eng
http://dentistry3000.pitt.edu/ojs/dentistry3000/article/view/267/146
Copyright (c) 2022 Mahdjoube Goldani Moghadam, Masoud Yousefi, Aylin Bagherzade, Fateme Ghorbanzade, Mohammad Yahya Hanafi- Bojd
8683bd044c91587f795a31856beaa991