Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

Authors

  • Aamir Zahid Godil M. A. Rangoonwala College of Dental Sciences and Research Centre
  • Diksha Bhagat Savitribai Phule Pune University
  • Parijat Das Savitribai Phule Pune University
  • Ashi Ilyas Kazi M A Rangoonwala College of Dental Sciences and Research Centre
  • Ramandeep Dugal M. A. Rangoonwala College of Dental Sciences and Research Centre
  • Surekha Satpute Savitribai Phule Pune University

DOI:

https://doi.org/10.5195/d3000.2021.120

Keywords:

Antifungal agents, biofilms, scanning electron microscope, soft liners, hardness, surface roughness, minimal inhibitory concentration, denture stomatitis

Abstract

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.

Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).

Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.

Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.

Author Biographies

Aamir Zahid Godil, M. A. Rangoonwala College of Dental Sciences and Research Centre

MDS, Senior Lecturer, Department of Prosthodontics, M. A. Rangoonwala College of Dental Sciences and Research Centre, Pune, Maharashtra, India

Diksha Bhagat, Savitribai Phule Pune University

Department of Microbiology, Savitribai Phule Pune University, Pune - 411007, India

Parijat Das, Savitribai Phule Pune University

Protein Crystallography Lab (603), Department of Biosciences and Bioengineering, IIT, Mumbai - 400076, Maharashtra, India.

Ashi Ilyas Kazi, M A Rangoonwala College of Dental Sciences and Research Centre

Post-graduate Resident, Department of Prosthodontics and Implantology, M. A. Rangoonwala College of Dental Sciences and Research Centre, Pune - 411001 Maharashtra, India

Ramandeep Dugal, M. A. Rangoonwala College of Dental Sciences and Research Centre

MDS, Professor and Head, Department of Prosthodontics, M. A. Rangoonwala College of Dental Sciences and Research Centre, Pune, Maharashtra, India

Surekha Satpute, Savitribai Phule Pune University

Assistant Professor, Department of Microbiology, Savitribai Phule Pune University, Pune - 411007, India

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Published

2021-02-05

Issue

Vol. 9 No. 1 (2021)

Section

Adults & the Elderly

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